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pJH4783

Plasmid

#200785

Purpose

Pnpr-4 snb-1::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of snb pHluorin

Depositor

Mei Zhen

Publication

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

snb-1 (snb-1 Nematode)

Tags

pHiuorin

Expression

Worm

Promoter

Pnpr-4

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSF11-wmiRFP670-2-T2A-HO1

Plasmid

#197250

Purpose

Expresses the protein of wmiRFP670-2-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Publication

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmiRFP670-2-T2A-HO1

Expression

Worm

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pNMSB84

Plasmid

#199309

Purpose

MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

Depositor

Michael Krieg

Publication

Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

Insert

flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR

Expression

Worm

Mutation

mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…

Promoter

flp-18

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pHygRR2r

Plasmid

#200076

Purpose

hygR selection mScarlet 2X marked rapid RMCE integration vector

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pHygCG2

Plasmid

#200073

Purpose

hygR selection mNG marked rapid RMCE integration vector

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pJR014

Plasmid

#198813

Purpose

Natural light-gated anion channels. In vivo inhibition of neurons using blue light.

Depositor

Paul Sternberg

Publication

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::GtACR2::SL2::GFP::let-858 3'UTR

Expression

Worm

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pJH2685

Plasmid

#191372

Purpose

Punc-4-TeTx::mCherry unc-54 3' UTR C.elegans A MNs/others neurou expression of TeTx RFP

Depositor

Mei Zhen

Publication

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Tetanus Toxin Light Chain

Tags

wCherry

Expression

Worm

Promoter

Punc-4

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pEY14

Plasmid

#191059

Purpose

acr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acr-5(orf) (acr-5 Nematode)

Expression

Worm

Mutation

None

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEY12

Plasmid

#191018

Purpose

Fluorescent reporter driving panneuronal nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

rab-3 (rab-3 Nematode)

Expression

Worm

Mutation

None

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY41

Plasmid

#191045

Purpose

dat-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

dat-1 (dat-1 Nematode)

Expression

Worm

Mutation

None

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJW2341

Plasmid

#186950

Purpose

30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker

Depositor

Jordan Ward

Publication

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker

Use

CRISPR

Expression

Worm

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2

Plasmid

#182359

Purpose

Positive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Ultra-pan-neuronal positive control pUNP::gfp(NLS)

Expression

Worm

Promoter

Promoter Ultra Pan Neuronal

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2

Plasmid

#182350

Purpose

Ubiquitous expression cloning vector for mosTI(unc-119). Transgene inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2

Plasmid

#182351

Purpose

Germline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJH4723

Plasmid

#178899

Purpose

Pges-1 DAF-2 unc-54 3' UTR C.elegans gut expression of of DAF-2

Depositor

Mei Zhen

Publication

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-2 (daf-2 Nematode)

Expression

Worm

Promoter

Pges-1

Available since

Jan. 5, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pJH4733

Plasmid

#178898

Purpose

Pmyo-3 DAF-2 unc-54 3' UTR C.elegans muscle expression of DAF-2

Depositor

Mei Zhen

Publication

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-2 (daf-2 Nematode)

Expression

Worm

Promoter

Pmyo-3

Available since

Jan. 5, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pJH3377

Plasmid

#175286

Purpose

Pges-1 DAF-16d/f unc-54 3' UTR C.elegans gut expression of isoforms d/f of DAF-16

Depositor

Mei Zhen

Publication

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

daf-16 d/f (daf-16 Nematode)

Expression

Worm

Promoter

Pges-1

Available since

Nov. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pJRK249

Plasmid

#173747

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Publication

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::CRY2(olig)::VHH(mCherry)::unc-54 3' UTR

Expression

Worm

Promoter

Prps-0

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJN63 [pmyo-3::BeCyclOp::SL2::mCherry]

Plasmid

#168173

Purpose

Expression of BeCyclOp in BWMs of C. elegans

Depositor

Alexander Gottschalk

Publication

Gao et al Nat Commun. 2015 Sep 8;6:8046. doi: 10.1

Insert

BeCyclOp (codon optimized for C. elegans)

Expression

Worm

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJH126

Plasmid

#162482

Purpose

SEC plasmid containing LG1 homology arms and vha-8p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.

Depositor

Bob Goldstein

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

vha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

Use

CRISPR

Tags

vha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

Expression

Worm

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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