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pHKMC1: Empty sgRNA for Cloning

Plasmid

#67720

Purpose

Empty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.

Depositor

Monica Colaiacovo

Publication

Norris et al Genetics. 2015 Oct;201(2):449-58. doi: 1

Type

Empty backbone

Expression

Worm

Promoter

PU6

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pHIP_RNA1

Plasmid

#61259

Purpose

Orsay virus RNA1 genome segment expression plasmid for virus reverse genetics

Depositor

David Wang

Publication

Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

Insert

Orsay RNA1

Expression

Worm

Promoter

heat shock promoter

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJM483

Plasmid

#52987

Purpose

A secreted Lactadherin (sGFP::LactC1C2) Phosphatidylserine sensor

Depositor

Ding Xue

Publication

Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

Insert

Lactaherin (Mfge8 Mouse)

Tags

GFP (w/ introns) and signal sequence

Expression

Worm

Mutation

deleted native signal sequence, deleted C-termina…

Promoter

hsp-16.41

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pMB63

Plasmid

#47945

Purpose

Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoter

Depositor

Mike Boxem

Publication

Waaijers et al Genetics. 2013 Aug 26.

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and egl-13 NLS

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pBCN40-R4R3

Plasmid

#34915

Depositor

Ben Lehner

Publication

Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

Type

Empty backbone

Expression

Worm

Available since

March 21, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pPD96_32

Plasmid

#1504

Depositor

Andrew Fire

Type

Empty backbone

Tags

IVSA, ATG-MitLS, gfpN, IVSR, gfpS65C, IVSS, IVST,…

Expression

Worm

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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L3786

Plasmid

#1593

Depositor

Andrew Fire

Type

Empty backbone

Tags

let858enh>>, let858enh<<, let858-455,…

Expression

Worm

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pWXC077

Plasmid

#222960

Purpose

C. elegans codon-optimized mScarlet3 with 3 introns cloned into pPD95.75 backbone

Depositor

Oliver Hobert

Publication

Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

Insert

mScarlet3

Expression

Worm

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pSEM371 - ce-unc-119 array integration

Plasmid

#182640

Purpose

Co-injection fragment for extra-chromosomal array integration at ce-unc-119 locus

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Plasmid

#159813

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSEM320 - sgRNA mosTI unc-119 array insertion

Plasmid

#159824

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI unc-119 array insertion

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1586

Plasmid

#121057

Purpose

mKate2^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

mKate2-T^SEC^AID*::3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFLAG, C. elegans codon optimized mKate2, and au…

Expression

Worm

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCFJ150-GFP(dpiRNA)::CDK-1

Plasmid

#107938

Purpose

cdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.

Depositor

Heng-Chi Lee

Publication

Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

Insert

GFP(dpiRNA)::CDK-1 (cdk-1 Nematode)

Expression

Worm

Promoter

cdk-1

Available since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pBN267

Plasmid

#86723

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Publication

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Prgef-1::FLP D5

Expression

Worm

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBN41

Plasmid

#86716

Purpose

C. elegans injection marker

Depositor

Peter Askjaer

Publication

Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

Insert

Pmyo-2::GFP

Expression

Worm

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJA327

Plasmid

#74486

Purpose

superfolder GFP (myo-3::sfGFP::let-858, using backbone as in L3785)

Depositor

Andrew Fire

Publication

Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

Insert

superfolder GFP

Expression

Worm

Promoter

myo-3

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSR49

Plasmid

#69259

Purpose

phlh-8::tagBFP2

Depositor

Sander van den Heuvel

Publication

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

TagBFP2

Use

Cre/Lox

Expression

Worm

Mutation

codon-optimzed index 1.0, TagBFP2 contains 2 sing…

Promoter

hlh-8

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCFJ1663

Plasmid

#51484

Purpose

miniMos transposable element with HygroR and peel-1, MCS cloning

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Expression

Worm

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGC94

Plasmid

#19610

Depositor

Jane Hubbard

Publication

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Phsp-16.2::FLP::let-858(3’)

Expression

Worm

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCM5.36

Plasmid

#19394

Depositor

Geraldine Seydoux

Publication

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

gld-1 3'utr in pDONRP2RP3 (gld-1 Nematode)

Expression

Worm

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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