1,755 results

1. pSEM376 - sgRNA - spc4 - array - insertion - unc-119 - locus

   Plasmid

   #182344

   Purpose

   sgRNA4 for insertion of extrachromosomal arrays into the ce-unc-119 genomic locus. Use with pSEM371 fragment included in Ex array.

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   Spacer 4
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSDH54

   Plasmid

   #139052

   Purpose

   MosSCI SapTrap assembly: Cassette 4 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   tbb-2 3'UTR (tbb-2 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPD_Pinx-1::ChR2(H134R)::mCherry

   Plasmid

   #117420

   Purpose

   For selectively expressing ChR2(H134R) in AiB interneurons under the expression of a inx-1 promoter

   Depositor

   Shohei Mitani

   Article

   Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

   Insert

   channel rhodopsin-2 with H134R mutation
   

   Use

   Tags

   Expression

   Worm

   Mutation

   H134R

   Promoter

   inx-1

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEY11

   Plasmid

   #191017

   Purpose

   Fluorescent reporter driving panneuronal nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   Ultrapanneuronal
   

   Use

   Tags

   Expression

   Worm

   Mutation

   None

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEG345

   Plasmid

   #40077

   Depositor

   Geraldine Seydoux

   Article

   Griffin et al Cell. 2011 Sep 16;146(6):955-68.

   Insert

   Mex-5 (mex-5 Nematode)
   

   Use

   Tags

   Dendra2 PFAF, S-peptide, and TEV protease cleavag…

   Expression

   Worm

   Mutation

   Promoter

   pie-1

   Available since

   Sept. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pJK2020

   Plasmid

   #171932

   Purpose

   express MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operon

   Depositor

   Judith Kimble

   Article

   Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.

   Insert

   Pmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR
   

   Use

   Tags

   superfolder GFP, mCherry

   Expression

   Worm

   Mutation

   Promoter

   -

   Available since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Pmec-18 cz::caspase-3 (p17) [TU#814]

   Plasmid

   #16083

   Depositor

   Martin Chalfie

   Article

   Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

   Insert

   cz :: caspase-3 (p17) (CASP3 Human)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 23, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJJR82

   Plasmid

   #75027

   Purpose

   EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Mike Boxem

   Article

   Ramalho et al Development. 2020 Jul 22;147(14):dev1880

   Insert

   EGFP^SEC^3xFlag
   

   Use

   CRISPR

   Tags

   3xFLAG and C. elegans codon optimized EGFP

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCZGY2727

   Plasmid

   #193330

   Purpose

   Site specific insertion using CRISPR/Cas9 editing of C. elegans ChrI

   Depositor

   Yishi Jin

   Article

   Blazie et al Elife. 2021 Jul 29;10. pii: 68336. doi:

   Insert

   Hygromycin resistance
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Prps-0

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDD315 (mTurquoise2^SEC^2xHA)

    Plasmid

    #73343

    Purpose

    mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Zhang et al J Cell Biol. 2023 Sep 4;222(9):e20230210

    Insert

    mTurquoise2-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    2xHA and C. elegans codon-optimized mTurquoise2

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. TU#867 (Punc-119sid-1)

    Plasmid

    #25811

    Depositor

    Martin Chalfie

    Article

    Calixto et al Nat Methods. 2010 Jul;7(7):554-9. doi: 1

    Insert

    systemic RNA interference-deficient 1 (sid-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pNM3774

    Plasmid

    #154117

    Purpose

    tetO 7X enhancer with ∆mec-7 basal promoter driving GFP-C1

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    tetO 7X::∆mec-7p::GFP-C1::tbb-2 3'
    

    Use

    Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRab-3::Cerulean-Venus::Lgg-1

    Plasmid

    #68045

    Purpose

    Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in the neurons, for monitoring autophagy

    Depositor

    Dana Miller

    Article

    Chapin et al Aging (Albany NY). 2015 Jun 30.

    Insert

    pRab-3::Cerulean-Venus::Lgg-1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEM1 = flp-21::LoxPStopLoxP::npr-1 SL2 GFP

    Plasmid

    #24033

    Depositor

    Cori Bargmann

    Article

    Macosko et al Nature. 2009 Apr 30. 458(7242):1171-5.

    Insert

    Npr1 (npr-1 Nematode)
    

    Use

    Cre/Lox

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. L4417

    Plasmid

    #1649

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    RNAi

    Tags

    T7p, gfpC, gfpS65S, gfpN, T7p, lacZN, OriF1>&g…

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEY139

    Plasmid

    #223486

    Purpose

    tph-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    tph-1 (tph-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSX2719

    Plasmid

    #200763

    Purpose

    Expresses mCherry fused MS2 coat protein and 24xGCN4 (Suntag) with the tissue-specific promoter semo-1 in C. elegans.

    Depositor

    Suhong Xu

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    MCP-24xGCN4
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    semo-1

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSX2720

    Plasmid

    #200764

    Purpose

    Expresses Superfolder GFP-tag fused single change variable fragment antibody of GCN4 (Suntag) with the tissue-specific promoter col-19 in C. elegans.

    Depositor

    Suhong Xu

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    scFv-sfGFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    col-19

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEY82

    Plasmid

    #191077

    Purpose

    flp-7 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    flp-7 (flp-7 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCZGY847

    Plasmid

    #135093

    Purpose

    Transcriptional mcherry reporter of acr-2 (cholinergic) expressing neurons in C. elegans

    Depositor

    Yishi Jin

    Article

    Jospin et al PLoS Biol. 2009 Dec;7(12):e1000265. doi:

    Insert

    Pacr-2
    

    Use

    Tags

    mcherry and unc-10 3'UTR

    Expression

    Worm

    Mutation

    Promoter

    Pacr-2

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge