8,086 results

1. pPAP004

   Plasmid

   #160911

   Purpose

   E.coli/ P.pastoris shuttle plasmid for episomal expression in P.pastoris

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

   Insert

   lacZ selection cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFA6a-link-ymScarletI3-URA3

   Plasmid

   #224075

   Purpose

   Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with URA3 selection

   Depositor

   Bas Teusink

   Insert

   ymScarletI3
   

   Use

   Tags

   ymScarletI3

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEasyCas9

   Plasmid

   #184909

   Purpose

   Expresses the Streptococcus pyogenes Cas9 protein

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Cas9-NAT

   Plasmid

   #64329

   Purpose

   Cas9 expression cassette carried by a yeast single-copy episome plasmid

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GFP-ATG8(416)/GFP-AUT7(416)

   Plasmid

   #160389

   Purpose

   Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter , NAT selection

   Depositor

   John McCusker

   Insert

   autophagy-related 8 (ATG8 Budding Yeast)
   

   Use

   Cen/ars

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Endogenous ATG8 promoter

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS316-OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)

   Plasmid

   #198417

   Purpose

   Expresses both OsTIR1F74A and mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeast

   Depositor

   Takumi Kamura

   Article

   Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

   Insert

   OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 promoter

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUCC001

   Plasmid

   #124451

   Purpose

   Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   HH-BsaI
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   gRNA expression cassette from pUDP002 modified to…

   Promoter

   TDH3 promoter from S.cerevisiae (already present …

   Available Since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYDS2.0_AT118-L

   Plasmid

   #222847

   Purpose

   Yeast-display plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-L
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKT0127

   Plasmid

   #8728

   Depositor

   Kurt Thorn

   Article

   Sheff et al Yeast 2004 Jun;21(8):661-70.

   Insert

   yEGFP
   

   Use

   Tags

   Codon optimized linker

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAS

    Plasmid

    #60847

    Purpose

    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

    Depositor

    Jamie Cate

    Article

    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

    Inserts

    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    NLS/His8 and TTT 3' extension prior to sgRNA

    Expression

    Bacterial and Yeast

    Mutation

    L4 is UUCG tetraloop and guide targets LYP1 (CATA…

    Promoter

    Available Since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVW296

    Plasmid

    #139496

    Purpose

    Expression vector for the PP7-mCherry protein in S. cerevisiae

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pADH1 PP7∆FG-mCherry tCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ∆FG

    Promoter

    pADH1

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMTS-hFluc

    Plasmid

    #177716

    Purpose

    Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    hFluc
    

    Use

    Tags

    Mitochondrial Targeting Sequence MTS SS

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHLUM

    Plasmid

    #40276

    Depositor

    Markus Ralser

    Article

    Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

    Inserts

    LEU2 (LEU2 Budding Yeast)
    URA3 (URA3 Budding Yeast)
    MET15/17 (MET17 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    endogenous LEU2, endogenous MET15/17, and endogen…

    Available Since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSP568

    Plasmid

    #139493

    Purpose

    Insertion of the pSTL1 promoter and 24 MS2 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pSTL1 24xMS2sl
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    pSTL1

    Available Since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBBK88 pHomL-TDH3p-Neon(gfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)

    Plasmid

    #179072

    Purpose

    Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp)-Link-2xPH(PLCd), an overexpressed (bright) marker for the plasma membrane

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    TDH3p-Neon(gfp)-Link-2xPH(PLCd)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVW293

    Plasmid

    #139490

    Purpose

    Insertion of the pHSP12 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pHSP12 24xPP7sl
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    pHSP12

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCEV-G2-Km ymNeongreen

    Plasmid

    #193959

    Purpose

    TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymNeongreen in budding yeast

    Depositor

    Bas Teusink

    Insert

    ymNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA6a-6xGLY-3xFLAG-HIS3MX6

    Plasmid

    #20753

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p306-BrdU-Inc

    Plasmid

    #71792

    Purpose

    BrdU-incorporating plasmid with selectable marker URA3

    Depositor

    Oscar Aparicio

    Article

    Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

    Inserts

    Thymidine Kinase
    ENT1 (SLC29A1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 and GPD

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS306-URA3-PHIS3-AqLumSynth –Sapphire

    Plasmid

    #116931

    Purpose

    Yeast integrative vector for the exression of the 20nm GEM under the HIS3 promoter

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    lumazine synthase
    

    Use

    Tags

    Sapphire

    Expression

    Yeast

    Mutation

    Promoter

    HIS3

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge