8,060 results

1. pCfB2803

   Plasmid

   #63646

   Purpose

   pCfB2803 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

   Depositor

   Irina Borodina, Jerome Maury

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB9337 (pgRNA_IV-1_NatMX)

   Plasmid

   #161590

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1

   Depositor

   Irina Borodina

   Article

   Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

   Insert

   guiding RNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB2899(X-2 MarkerFree)

   Plasmid

   #73271

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJAS1052

   Plasmid

   #158598

   Purpose

   VioA, VioB

   Depositor

   John Dueber

   Article

   Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

   Inserts

   VioA
   VioB
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. YIplac211-APL2-iGFPx6

   Plasmid

   #105251

   Purpose

   Plasmid for pop-in/pop-out replacement of Apl2 with Apl2-iGFPx6.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   APL2 portion (APL2 Budding Yeast)
   

   Use

   Tags

   iGFPx6

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG413GAL-ccdB-TAP

   Plasmid

   #14261

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   TAP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. Coexpressed Free GFP-NES

   Plasmid

   #182491

   Purpose

   Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Inserts

   FPPS
   GFP-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10 and GAL7

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. YIplac211-Sec7-mScarlet2Ix6

   Plasmid

   #140151

   Purpose

   Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-mScarlet2Ix6

   Depositor

   Benjamin Glick

   Article

   Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

   Insert

   SEC7
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pILGFPU0 (R)

   Plasmid

   #83562

   Purpose

   Express yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoter

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

   Insert

   TEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBridge-tyrosinase.σ1A

    Plasmid

    #197381

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-1 σ1A
    

    Use

    Tags

    GAL4-DNA binding domain fragment, HA tag, and SV4…

    Expression

    Yeast

    Mutation

    Contains an extra 42 nucleotides encoding 14 resi…

    Promoter

    ADH1 and MET25

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. LD113

    Plasmid

    #69166

    Purpose

    Integration of GFP tag at Atg13 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg13-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPICZ-aC-KHC-BZ

    Plasmid

    #160427

    Purpose

    Expression of c-luciferase from sp. Kornikeria hastingsi carriebowae (Belize)

    Depositor

    Todd Oakley

    Article

    Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

    Insert

    luciferase
    

    Use

    Luciferase

    Tags

    6x Histidine tag, Yeast alpha secretion factor, c…

    Expression

    Yeast

    Mutation

    Promoter

    AOX

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRPR1(1xTetO)_gRNA_handle_RPR1t

    Plasmid

    #62966

    Purpose

    aTc inducible gRNA expression vector

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    RPR1

    Available since

    March 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSc-His6-Sem1Sc

    Plasmid

    #83235

    Purpose

    pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    Sem 1 (SEM1 Budding Yeast)
    

    Use

    Tags

    His6

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pG1-GR526

    Plasmid

    #1124

    Depositor

    Susan Lindquist

    Article

    Li et al Science 2000 Jan 28;287(5453):661-4.

    Insert

    GR526 (Nr3c1 Rat)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    GR 526 is a truncated, constitutively active form…

    Promoter

    Available since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

16. Padh1-OsTIR1(WT)-NLS-TADH1-Sp.arg3+/pUC19*

    Plasmid

    #169352

    Purpose

    Expression of OsTIR1(WT) in S. pombe from adh1 promoter, integrated at arg3-D4 locus

    Depositor

    Tony Carr

    Insert

    OsTIR1(WT)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMEL15

    Plasmid

    #107921

    Purpose

    nat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS415-KEX2

    Plasmid

    #10996

    Depositor

    Francisco Malagon

    Insert

    KEX2 (KEX2 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Wild type

    Promoter

    Available since

    Dec. 29, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. pK3F-GAL1

    Plasmid

    #85772

    Purpose

    N-ICE plasmid pK3F-GAL1

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-KanMX-loxP-GAL1p-3FLAG
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pH-eCFP

    Plasmid

    #176555

    Purpose

    Expression of eCFP for auxotrophic selection in the absence of histidine

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    eCFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH1(GAP)

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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