8,057 results

1. pDRF1 mScarletI3-T2A-mTurquoise2

   Plasmid

   #219845

   Purpose

   equimolar expression of mScarletI3 and mTurquoise2 in budding yeast

   Depositor

   Bas Teusink

   Article

   Botman et al bioRxiv 2025.06.27.661975; doi: https://

   Insert

   mScarletI3-T2A-mTurquoise2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pABY-T2

   Plasmid

   #201765

   Purpose

   For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

   Depositor

   Saravanan Palani

   Article

   Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

   Insert

   ALFA tag
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAD-IRP

   Plasmid

   #220751

   Purpose

   Plasmid expresses the rabbit Iron Regulatory Protein fused to the Gal 4 Activation Domain.

   Depositor

   Marvin Wickens

   Article

   Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

   Insert

   Iron Regulatory Protein (ACO1 )
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFA6a-link-ytdTomato-URA3

   Plasmid

   #168054

   Purpose

   Replaces Addgene plasmid 118461. Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ytdTomato
   

   Use

   Tags

   ytdTomato

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. ZJOM90

   Plasmid

   #133647

   Purpose

   Integration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   pTPI1-SS-mCherry-HDEL
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-link-yoEGFP-Kan

   Plasmid

   #44900

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized EGFP with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDB5320

   Plasmid

   #204830

   Purpose

   An integrating plasmid containing both the inducible enotetSW2 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

   Depositor

   Li-Lin Du

   Article

   Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

   Inserts

   tetR
   mECitrine
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CMV promoter and enotetSW2 promoter

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGBKCg

   Plasmid

   #20162

   Depositor

   Peter Uetz

   Article

   Stellberger et al Proteome Sci. 2010 Feb 15;8:8. doi: 10.1

   Type

   Empty backbone

   Use

   Tags

   Gal4 DBD

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTDH3-dCas9-Mxi1

   Plasmid

   #46921

   Purpose

   Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoter

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9-Mxi1
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSC-humanADAR1

    Plasmid

    #198235

    Purpose

    Expresses human ADAR1 in yeast

    Depositor

    Brenda Bass

    Article

    Eggington et al Nat Commun. 2011;2:319. doi: 10.1038/nco

    Insert

    human ADAR1 (ADAR Human)
    

    Use

    Tags

    His10_TEV

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAW240

    Plasmid

    #170791

    Purpose

    P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution

    Depositor

    Chang Liu

    Article

    Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

    Insert

    p10B2-AT110
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    10B2 (P1 specific promoter)

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. FRP880_PACT1(-1-520)-LexA-ER-haB112-TCYC1

    Plasmid

    #58437

    Purpose

    Expresses LexA-ER-B112 in yeast cells

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    LexA-ER-B112
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Act1 promoter (-520;1)

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBIS-GALkFLP(Trp1)

    Plasmid

    #49459

    Purpose

    overespression of Flp step-arrest mutant in yeast, TRP1 selection

    Depositor

    Marc Gartenberg

    Article

    Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

    Insert

    Flp(H305L) (flp Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H305L, ‘step-arrest’ mutant,

    Promoter

    GAL10

    Available since

    Nov. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDB5321

    Plasmid

    #204831

    Purpose

    An integrating plasmid containing both the inducible enotetSW3 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

    Depositor

    Li-Lin Du

    Article

    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

    Inserts

    tetR
    mECitrine
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CMV promoter and enotetSW3 promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGADCg

    Plasmid

    #20161

    Depositor

    Peter Uetz

    Article

    Stellberger et al Proteome Sci. 2010 Feb 15;8:8. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    Gal4 AD and SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYDS2.0_AT118-H

    Plasmid

    #222846

    Purpose

    Yeast-display plasmid encoding Nb. AT118-H, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-H
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TetR in pENTR1A (559-1)

    Plasmid

    #22265

    Depositor

    Eric Campeau

    Insert

    Tetracycline repressor (tetR )
    

    Use

    Tags

    Expression

    Insect, Mammalian, Worm Exp…

    Mutation

    Promoter

    Available since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA6a-link-yemiRFP670-Kan

    Plasmid

    #224088

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized emiRFP670 with Kan selection

    Depositor

    Bas Teusink

    Insert

    yemiRFP670
    

    Use

    Tags

    yemiRFP670

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGR475

    Plasmid

    #213784

    Purpose

    Integrates at the CAN1 locus the WT TP-DNAP1 and the Hygromycin resistance marker

    Depositor

    Chang Liu

    Insert

    WT TP-DNAP1 (recoded)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFA6a-HaloTag-KanMX6

    Plasmid

    #87029

    Purpose

    Tag S. pombe genes in C terminal with HaloTag

    Depositor

    Julien Berro

    Insert

    HaloTag
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge