8,432 results
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Plasmid#160936PurposeIn vivo mRNA display construct for GFPDepositorInsertMCP-sfGFP-HIS tag, 3'UTR MS2 Stem Loop
TagsHIS tag, 3'UTR MS2 Stem LoopExpressionYeastPromoterMET25Available sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD_85
Plasmid#149604Purpose(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - XylRDepositorInsertXylR
UseSynthetic BiologyTagsSV40ExpressionYeastPromoterTEF1Available sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL28-decoder
Plasmid#159450PurposeInsert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.DepositorInsertPlexAtet-LexA-ER-B112-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPlexAtetAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL66-decoder
Plasmid#159453PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1mutAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pI2-MTU-DO-HPH
Plasmid#160019PurposeIntegrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Hygromycin selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pI2-MTU-DO-LEU
Plasmid#160017PurposeIntegrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Leucine selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRG711_lexO-AscI_LexA-TF_HIS3MX
Plasmid#154820PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
TagsFLAG and NLSExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSG830
Plasmid#158601PurposetNCS-ePTS1 (2micron)DepositorInsertpTDH3-tNCS-ePTS1
TagsePTS1ExpressionYeastAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSG465
Plasmid#158587PurposeEnzymes Bv-CYP76AD5, DODC, ARO4_K229LDepositorInsertsBv-CYP76AD5
DODC
ARO4_K229L
ExpressionYeastMutationK229LAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSJD001
Plasmid#158589PurposePex22(1-36)-mRuby2 peroxisome markerDepositorInsertPex22(1-36)-mRuby2
ExpressionYeastAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJAS1669
Plasmid#158594PurposeVioE-dead_ePTS1 CEN6/ARS4DepositorInsertVioE-dead_ePTS1
ExpressionYeastAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICE ADE2-HIS3
Plasmid#122883PurposeADE2-HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3
Plasmid#112651PurposeExpression S.cerevisiaeDepositorInsertGlo3
ExpressionYeastPromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3
Plasmid#112658PurposeExpression S.cerevisiaeDepositorInsertsfGFP-Glo3
TagssfGFPExpressionYeastPromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1
Plasmid#112646PurposeExpression S.cerevisiaeDepositorInsertGcs1
ExpressionYeastPromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT45_GalL_nCDA1-BE3
Plasmid#145038PurposeExpresses nCDA1-BE3 in yeast cellsDepositorInsertnCDA1-BE3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRP1864
Plasmid#159307PurposeYeast tagging vector to create gene fusion with yeast codon optimized tdKOkappa under KanMX selectionDepositorTypeEmpty backboneExpressionYeastAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRP272
Plasmid#159305PurposeYeast tagging vector to create gene fusion with sfGFP under KanMX selectionDepositorTypeEmpty backboneExpressionYeastAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRP322
Plasmid#159303PurposeYeast tagging vector to create gene fusion with yeast codon optimized CitrineA206K under KanMX selectionDepositorTypeEmpty backboneExpressionYeastMutationV2del, S3del, F64L, A206K, L231HAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCLASP_Crz1(19A)_CLASP_RGS2membrane
Plasmid#133085PurposePlasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.DepositorInsertCrz1*-CLASP
ExpressionBacterial and YeastPromoterpADH1Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only