8,057 results

1. MET-HYGRO

   Plasmid

   #218113

   Purpose

   Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LYS-Leu2

   Plasmid

   #218114

   Purpose

   Integration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pWS158

   Plasmid

   #90517

   Purpose

   Cas9 gap repair vector - URA3

   Depositor

   Tom Ellis

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNIA-CEN-FLAG-LINXa4(RVH)-Bre1

   Plasmid

   #116885

   Purpose

   FLAG-tagged LINXa4-Bre1 with RVH hinge-loop mutations

   Depositor

   Brian Kuhlman

   Article

   Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

   Insert

   LINXa4(RVH)-Bre1
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

   Promoter

   ADH1

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMK425 (GAL-OsTIR1(F74A))

   Plasmid

   #140657

   Purpose

   GAL-OsTIR1(F74A)

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   GAL-OsTIR1(F74A)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACT2-Kre33

   Plasmid

   #99990

   Purpose

   Express yeast Kre33 with Y2H activation domain fusion

   Depositor

   Susan Baserga

   Article

   Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

   Insert

   Kre33 (KRE33 Budding Yeast)
   

   Use

   Tags

   Y2H activation domain

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. LT2_7_OxPro

   Plasmid

   #177709

   Purpose

   Plasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Inserts

   pTRX2_5xUAS-ymYPET-tCYC1
   pTEFmut8-mCherry-tADH1
   

   Use

   Used as donor dna in genome integration after lin…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pV1200

   Plasmid

   #111429

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al Sci Adv. 2015;1(3):e1500248.

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGAL-GFP kan

   Plasmid

   #122060

   Purpose

   galactose inducible expression of GFP in yeast. marked with URA3

   Depositor

   Daniel Reines

   Article

   Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

   Insert

   green fluorescent protein
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMT899

    Plasmid

    #228288

    Purpose

    Constitutive mutant rPhlTA expression with strong promoter

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    rphlTA mutant
    

    Use

    Synthetic Biology

    Tags

    Three copies of VP16 (VP48)-Nuclear localization …

    Expression

    Yeast

    Mutation

    P5S, S6P, K86T, F109L, Q117R, E143K

    Promoter

    KpGAPDH promoter

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-link-yoLSS-mKate2-SpHis5

    Plasmid

    #44848

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with SpHis5 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoLSS-mKate2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAG426GAL-EGFP-ccdB

    Plasmid

    #14323

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHES262 [prADH1-Msn2-mCherry, pnh604]

    Plasmid

    #103844

    Purpose

    Yeast Expression Plasmid

    Depositor

    Hana El-Samad

    Article

    Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

    Insert

    pAdh1-Msn2-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAG416GPD-ccdB

    Plasmid

    #14148

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHLUK

    Plasmid

    #64181

    Purpose

    harbours marker genes HIS3, LEU2, URA3 and LYS2

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS410

    Plasmid

    #11258

    Depositor

    Fred Cross

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-hphMX6-81nmt1

    Plasmid

    #105164

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

    Insert

    hphMX6-81nmt1
    

    Use

    Tags

    81nmt1

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    81nmt1

    Available since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA6a-hphMX6-41nmt1

    Plasmid

    #105163

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

    Insert

    hphMX6-41nmt1
    

    Use

    Tags

    41nmt1

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGBW-m4046159

    Plasmid

    #145509

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 endoRNAse

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 endoRNAse (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pFA6a-hphMX6-3nmt1

    Plasmid

    #105162

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

    Insert

    hphMX6-3nmt1
    

    Use

    Tags

    3nmt1

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    3nmt1

    Available since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge