7,943 results

1. pFA6a-6xGLY-HSV-hphMX4

   Plasmid

   #20776

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis

   Plasmid

   #124278

   Purpose

   Expresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

   Depositor

   Arthur Laganowsky

   Article

   Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

   Insert

   GIRK2 (Kcnj6 Mouse)
   

   Use

   Tags

   eGFP-StreptagII-6xHis

   Expression

   Yeast

   Mutation

   R201A

   Promoter

   AOX1

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. p416GAL1-Zipp.-L-IFP-F[1] (Yeast)

   Plasmid

   #52895

   Purpose

   Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   Zipp.-L-IFP-F[1]
   

   Use

   Tags

   L-IFP-F[1]

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pFA6-mTFP1-His3MX6

   Plasmid

   #69850

   Purpose

   C-terminal tagging with mTFP1 using His3MX6 as a selection marker in S. cerevisiae.

   Depositor

   Liza Pon

   Article

   Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

   Insert

   mTFP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRBUBR1

   Plasmid

   #20198

   Depositor

   Alexander Varshavsky

   Article

   Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

   Insert

   UBR1 (UBR1 Budding Yeast)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pICE ADE2-KanMx

   Plasmid

   #122896

   Purpose

   ADE2-KanMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-link-mDsRed-SpHis5

   Plasmid

   #44639

   Purpose

   Yeast tagging vector to create gene fusion with mDsRed with SpHis5 selection

   Depositor

   Kurt Thorn

   Insert

   mDsRed
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. myc ATG18

   Plasmid

   #42530

   Depositor

   James Hurley

   Article

   Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

   Insert

   ATG18 (ATG18 Budding Yeast)
   

   Use

   Tags

   myc

   Expression

   Yeast

   Mutation

   none

   Promoter

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

9. THI VP64 pGAP T5

   Plasmid

   #161492

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting T5 in the THI11 promoter)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pGAP, pPOR1, and pTEF2

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMS22H

    Plasmid

    #133904

    Purpose

    Expression of MS2BS-RNAsite hybrid. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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