8,400 results
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Plasmid#106150PurposeEasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying hygromycin resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, amp resistanceDepositorInsertCas9
ExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
p413_Csy4NLS
Plasmid#103139PurposeExpresses Csy4-NLS under TEF promoterDepositorInsertCsy4
TagsNLSExpressionYeastPromoterTEFAvailable SinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4282
Plasmid#98701PurposeA plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
bRA77
Plasmid#100953PurposeGAL1 driven Cas9. gRNA can be cloned into BplI sites.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRL_GI_PCB28
Plasmid#98745PurposeFor phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.DepositorInsertsUseSynthetic BiologyExpressionYeastMutationCodon optimized for Saccharomyces cerevisiaePromoterADH1m and PGK1Available SinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
SIVh
Plasmid#81091PurposeSingle Integration Vector HIS - Cloning vector for single integration into the HIS3 locus of S.cerevisiae containing two MCS.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRG203MX
Plasmid#64521PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJZC638
Plasmid#62283PurposeExpresses MCP-VP64 and dCas9 in Yeast cellsDepositorInsertsMCP-VP64
dCas9
TagsVP64ExpressionYeastMutationNuclease activity has been deactivated by mutatio…PromoterpAdh and pGal10Available SinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pTEF1p-CYC1t
Plasmid#62190PurposeExpresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionYeastMutationhuman codon-optimized, D10A, H840APromoterTEF1 promoterAvailable SinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
Plasmid#58440PurposeCassette to replace yeast promoters with an inducible promoter containing 2 lexA boxesDepositorInsertPTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
ExpressionYeastPromoterPTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCCL-ReDNAP (Y427A)
Plasmid#52346PurposeCEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)DepositorInsertRecoded TP-DNAP
ExpressionBacterial and YeastPromoterREV1Available SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoLSS-mKate2-Kan
Plasmid#44943PurposeYeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with Kan selectionDepositorInsertyoLSS-mKate2
ExpressionYeastAvailable SinceOct. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKO2-Kan
Plasmid#44958PurposeYeast tagging vector to create gene fusion with yeast optimized mKO2 with Kan selectionDepositorInsertyomKO2
ExpressionYeastAvailable SinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKT127-SEP
Plasmid#41899DepositorTypeEmpty backboneTagssuperecliptic GFP (SEP)ExpressionYeastAvailable SinceApril 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSM1960
Plasmid#41842DepositorInsertSEC63-mRFP
TagsmRFPExpressionYeastAvailable SinceJan. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTH761-CEN-mCherry_v4
Plasmid#40599DepositorInsertmCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
ExpressionYeastMutationmCherry ORF partially codon optimised for yeast e…PromoterGPD (TDH3)Available SinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal TDP43 WT
Plasmid#27458DepositorInsertTDP-43 (TARDBP Human)
ExpressionYeastAvailable SinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-Ago1
Plasmid#22306DepositorInsertAGO1
Use2micronExpressionYeastAvailable SinceOct. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA-Ub-G76A-Rnq1-GFP
Plasmid#21052DepositorAvailable SinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
GAL 103Q+ProGFPp416
Plasmid#15583DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only