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Plasmid#58435PurposeInduces Citrine expression from a promoter containing 8 lexA boxes in yeast cellsDepositorInsertCitrineA206K
UseTagsExpressionYeastMutationPromoterinsul-(lexA-box)8-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB8644
Plasmid#126919PurposePlasmid containing integration cassette at IntC2 integration site in Yarrowia lipolytica for overexpression of XdCrtI and XdCrtYBDepositorInsertXdCrtI and HMG1
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TID-HA3-HIS3MX6
Plasmid#227981PurposeTo insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, HIS3MX6 markerDepositorInsertTurboID
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG413GAL-ccdB
Plasmid#14141DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS426GFP-2ĆPH(PLCĪ“)
Plasmid#36092DepositorInsert2xPH(PLCdelta) fusion
UseTagsGFPExpressionYeastMutationcontains aa11-140 of PLCĪ“ (including the PH domaiā¦PromoterPRC1Available sinceJune 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pZS157 CRISPEY RT/Cas9
Plasmid#114454PurposeYeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expressionDepositorInsertSpCas9, Ec86-RT
UseCRISPRTagsSV40-NLSExpressionYeastMutationS. cerevisiae codon optimizedPromoterGAL1-GAL10Available sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS423-CUP1-His-Ubi
Plasmid#99541PurposeYeast episomal vector for expression of His-tagged ubiquitin; HIS3 markerDepositorInsertUBI3(1-76) (RPL40A Budding Yeast)
UseTags7xHisExpressionYeastMutationPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMEG_YLCas9_empty
Plasmid#117826PurposeCRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolyticaDepositorInsertYlpTEF_hCas9_YlCyc1TT
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_D17_hrGFP
Plasmid#84616PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into D17 locusDepositorInserthrGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW AT1.03YEMK
Plasmid#28004DepositorInsertAT1.03YEMK
UseTagsmVenus and mseCFPExpressionYeastMutationM60Y, G131E, K132M, 133KPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGal4AD-CIB1
Plasmid#28245PurposeExpresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activationDepositorUseTagsCIB1ExpressionYeastMutationPromoterADH1Available sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
MmSphk1
Plasmid#118599PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in miceDepositorInsertMus musculus sphingosine kinase 1
UseTagsFLAGExpressionYeastMutationPromoterGAL1, 10Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsSPHK1
Plasmid#118091PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)DepositorInsertHomo sapiens Sphingosine Kinase 1
UseTagsFLAGExpressionYeastMutationPromoterGAL1, 10Available sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G4-Km
Plasmid#46819PurposeTEF1 and HXT7 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and HXT7Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPing
Plasmid#47100PurposeExpresses the Ping open reading frame 1 (ORF1) and transposase from rice to allow mPing movement. The vector contains ORF1, transposase, mPing element and hph for hygromycin selection.DepositorInsertsPing cDNA
mPing
hygromycin resistance gene
UsePlant expressionTagsGFPExpressionYeastMutationPromoterCaMV35s and StUbi3Available sinceSept. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-ccdB-HA
Plasmid#14252DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYES2/103Q
Plasmid#1385DepositorInserthuntingtin (103Q) (HTT Human)
UseTagsEGFP and FLAGExpressionYeastMutationExon1 sequences containing the first 17 amino aciā¦PromoterAvailable sinceFeb. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pH-tdGFP
Plasmid#74322PurposepH stable tandem dimeric sfGFP variant (N149Y,Q204H) in pFA6a-URA3MX. Codon optimized for S. cerevisiaeDepositorInsertpH-tdGFP
UseYeast taggingTagsnoneExpressionYeastMutationN149Y, Q204HPromoterAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-yoHaloTag-TLG1
Plasmid#105277PurposePlasmid for pop-in/pop-out replacement of Tlg1 with yoHaloTag-Tlg1.DepositorInsertTlg1 (TLG1 Budding Yeast)
UseTagsyoHaloTagExpressionYeastMutationHaloTag has yeast optimized codonsPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG303GAL-ccdB
Plasmid#14133DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only