8,057 results

1. pBEVY-GL

   Plasmid

   #51225

   Purpose

   bi-directional expression vector for yeast, galactose inducible, LEU2 selection

   Depositor

   Charles Miller

   Article

   Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1,10

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMW37: pRS426-R2Bm RNA

   Plasmid

   #209291

   Purpose

   Contains the R2Bm non-LTR retrotransposon 5'UTR and 3'UTR sequences

   Depositor

   Feng Zhang

   Article

   Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

   Insert

   R2Bm 5'UTR and 3'UTR
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   GAL1 GAPDH

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWZ699

   Plasmid

   #208117

   Purpose

   Payload yeast assembly vector with TK-mActb on backbone

   Depositor

   Jef Boeke

   Article

   Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

   Type

   Empty backbone

   Use

   Synthetic Biology; Copy number inducible

   Tags

   Expression

   Bacterial, Mammalian, and Y…

   Mutation

   Promoter

   Human PGK1 for thymidine kinase

   Available since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-link-ymScarletI-SpHis5

   Plasmid

   #219844

   Purpose

   tagging vector to tag genes with ymScarletI and a HIS5 gene

   Depositor

   Bas Teusink

   Insert

   ymScarletI-SpHis5
   

   Use

   Tags

   ymScarletI

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pOGS539_haPD-1

   Plasmid

   #226716

   Purpose

   Plasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   High affinity PD-1 microbody
   

   Use

   Tags

   HA tag and Mating factor alpha secretion signal

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOGS539_rPD-L1nb

   Plasmid

   #226717

   Purpose

   Plasmid enabling yeast-mediated expression and secretion of recombinant PD-L1 nanobody (rPD-L1nb)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   Recombinant PD-L1 nanobody
   

   Use

   Tags

   HA tag, Histidine tag, and Mating factor alpha se…

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPRyl_MFE1

   Plasmid

   #84612

   Purpose

   Introduces DSB at MFE1 locus in Y. lipolytica

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   UAS1B8-TEF(136)

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDIV270

   Plasmid

   #226181

   Purpose

   Expresses Cas9 and gRNA targeting KU70 gene in K. phaffii.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

   Insert

   tRNA-sgRNA-tRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TEF1p (Komagataella phaffii)

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-GFP11-His3MX

   Plasmid

   #91951

   Purpose

   Universal C-terminal GFP11 tagging

   Depositor

   Rong Li

   Article

   Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPRyl_A08

    Plasmid

    #84610

    Purpose

    Introduces DSB at A08 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pG/N795

    Plasmid

    #108220

    Purpose

    Yeast expression vector for rat glucocorticoid receptor

    Depositor

    Keith Yamamoto, Didier Picard

    Article

    Schena et al Methods Enzymol. 1991;194:389-98.

    Insert

    Rat glucocorticoid receptor
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Sept. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS314-T7-GFP

    Plasmid

    #33130

    Depositor

    Michael Rosbash

    Article

    Dower et al RNA. 2002 May;8(5):686-97.

    Insert

    GFP with TDH3 5' and 3' UTR (gfp )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    T7

    Available since

    Dec. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFA6a-TID-HA3-HIS3MX6

    Plasmid

    #227981

    Purpose

    To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDuRCC-K

    Plasmid

    #81193

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below., Please view…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-link-ymTurquoise2-Kan

    Plasmid

    #224092

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with Kan selection

    Depositor

    Bas Teusink

    Insert

    ymTurquoise2
    

    Use

    Tags

    ymTurquoise2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVT100U-mtGFP

    Plasmid

    #45054

    Depositor

    Benedikt Westermann

    Article

    Westermann et al Yeast. 2000 Nov;16(15):1421-7.

    Insert

    mtGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBHM2404

    Plasmid

    #173441

    Purpose

    pRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR template

    Depositor

    Hiten Madhani

    Article

    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

    Insert

    mNeonGreen-CBP-2XFLAG-NAT1
    

    Use

    Tags

    CBP-2xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDRF1-GW ymNeongreen-T2A-mCherry

    Plasmid

    #125700

    Purpose

    Produces equimolar expression of yeast codon optimized mNeongreen and mCherry

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymNeongreen-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDRF1 mBaojin-T2A-mCherry

    Plasmid

    #219842

    Purpose

    equimolar expression of mBaojin and mCherry in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2025.06.27.661975; doi: https://

    Insert

    mBaojin-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pILFPQ13BE5

    Plasmid

    #223836

    Purpose

    Expressing a cytosolic cAMP sensor (mScarletI3-ApacH-StayGold2) in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Insert

    pTEF1>mScarletI3>ApacH>StayGold2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge