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pTEF:ATP

Plasmid

#92179

Purpose

HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.

Depositor

Matthias Heinemann

Publication

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Insert

ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)

Use

Integration and expression into yeast.

Tags

Yeast codon optimized mseCFP (donor) and cp173-mV…

Expression

Yeast

Promoter

TEF1p

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSJ1602 (pRS315-NOP1pr-mCherry-SCS2TM-GFP11)

Plasmid

#86417

Purpose

luminal split GFP11 reporter with mCherry

Depositor

Sue Jaspersen

Publication

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-mCherry-SCS2TM-GFP11

Tags

GFP11 and mCherry

Expression

Yeast

Promoter

NOP1

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSJ2039 (pRS316-NOP1pr-GFP1-10-SCS2TM)

Plasmid

#86418

Purpose

ONM/ER split GFP1-10

Depositor

Sue Jaspersen

Publication

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-GFP1-10-Scs2TM

Tags

GFP1-10

Expression

Yeast

Promoter

NOP1

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFM689

Plasmid

#74635

Purpose

alias pFA6a-mCherry-hphNT1 - vector for C-terminal tagging of yeast genes with mCherry and hygromycin selection marker

Depositor

Jiří Hašek

Publication

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Inserts

Expression

Yeast

Promoter

none

Available since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRG205

Plasmid

#64524

Purpose

Integrative E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Publication

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Expression

Yeast

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCT

Plasmid

#60620

Purpose

Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

Depositor

Huimin Zhao

Publication

Bao et al ACS Synth Biol. 2014 Sep 19.

Inserts

iCas9
tracrRNA

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Yeast

Mutation

changed Aspartate 147 to Tyrosine, Proline 411 to…

Promoter

RPR1p and TEF1p

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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M4786 KanMX::hisG-URA3-HisG Disruptor Converter

Plasmid

#51690

Purpose

Yeast marker swap plasmid for converting KanMX3 to hisG-URA3-hisG

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

hisG-URA3-hisG (URA3 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pICXNHG3

Plasmid

#49021

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Publication

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Promoter

AOX1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pICXC3GH

Plasmid

#49023

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Publication

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Promoter

AOX1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoClover-Kan

Plasmid

#44952

Purpose

Yeast tagging vector to create gene fusion with yeast optimized Clover with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoClover

Expression

Yeast

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pCYC2

Plasmid

#43970

Depositor

Hal Alper

Publication

Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Promoter

CYC1

Available since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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416Gal-FUS-P525L-YFP

Plasmid

#29628

Depositor

Aaron Gitler

Publication

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

P525L

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pE1c

Plasmid

#17461

Purpose

modified pENTR vector for C-terminal STREP tag fusion with your gene of interest

Depositor

Giovanna Benvenuto

Publication

Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

Type

Empty backbone

Use

Modified gateway entry vector

Tags

STREP

Expression

Bacterial, Insect, Mammalia…

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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GAL FLAG25QDProCFPp303

Plasmid

#15584

Depositor

Susan Lindquist

Publication

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q Delta Pro (HTT Human)

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAG423GAL-ccdB-HA

Plasmid

#14245

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

3HA

Expression

Yeast

Available since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

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pAG415GPD-ccdB-EGFP

Plasmid

#14194

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Yeast

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAG416GPD-ccdB-EGFP

Plasmid

#14196

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Yeast

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pKT0209

Plasmid

#8730

Depositor

Kurt Thorn

Publication

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yEGFP

Tags

Codon optimized linker

Expression

Yeast

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGPD-luxAB(HIS)

Plasmid

#1106

Depositor

Susan Lindquist

Publication

Parsell et al Nature 1994 Dec 1;372(6505):475-8.

Insert

bacterial luciferase

Expression

Yeast

Mutation

n/a

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pG1-GR526

Plasmid

#1124

Depositor

Susan Lindquist

Publication

Li et al Science 2000 Jan 28;287(5453):661-4.

Insert

GR526 (Nr3c1 Rat)

Expression

Yeast

Mutation

GR 526 is a truncated, constitutively active form…

Available since

June 3, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article