8,086 results

1. MET-HYGRO

   Plasmid

   #218113

   Purpose

   Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. LYS-Leu2

   Plasmid

   #218114

   Purpose

   Integration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRR-AR

   Plasmid

   #73045

   Purpose

   Constitutively expresses the androgen receptor, which is required for the activation of the promoter in pLAREG (Addgene plasmid #73045)

   Depositor

   David McMillen

   Article

   Mazumder et al Nucleic Acids Res. 2014 Aug;42(14):9514-

   Insert

   Androgen receptor (AR Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   please see depositor comments below

   Promoter

   glyceraldehyde phosphate dehydrogenase (GPD)

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. p406GAL1

   Plasmid

   #17437

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   GAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   GAL1 promoter is between SacI and XbaI restrictio…

   Promoter

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-GFP(S65T)-His3MX6

   Plasmid

   #41598

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   His3
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available Since

   May 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUDP002

   Plasmid

   #103872

   Purpose

   Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Inserts

   hph
   Spcas9 D147Y P411T
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Yeast

   Mutation

   D147Y P411T

   Promoter

   Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. ymScarlet

   Plasmid

   #111917

   Purpose

   yeast optimized mScarlet

   Depositor

   Patrick Lajoie

   Article

   Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

   Insert

   ymScarlet
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG415GAL-ccdB

   Plasmid

   #14145

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYES2/25Q

   Plasmid

   #1384

   Depositor

   Michael Sherman

   Article

   Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

   Insert

   huntingtin (25Q) (HTT Human)
   

   Use

   Tags

   EGFP and FLAG

   Expression

   Yeast

   Mutation

   Exon1 sequences containing the first 17 amino aci…

   Promoter

   Available Since

   Feb. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRS426 Gal-RNQ1-YFP

    Plasmid

    #18686

    Depositor

    Susan Lindquist

    Article

    Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

    Insert

    Rnq1 (RNQ1 Budding Yeast)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

11. p2B-55

    Plasmid

    #177924

    Purpose

    Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter

    Depositor

    Vadim Gladyshev

    Article

    Barre et al (unpublished)

    Insert

    Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    V9I; F67S; G72P, F86Y; R171K

    Promoter

    TDH3

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYZ462

    Plasmid

    #187970

    Purpose

    TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFM689

    Plasmid

    #74635

    Purpose

    alias pFA6a-mCherry-hphNT1 - vector for C-terminal tagging of yeast genes with mCherry and hygromycin selection marker

    Depositor

    Jiří Hašek

    Article

    Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

    Inserts

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available Since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ZJOM151

    Plasmid

    #133643

    Purpose

    Integration of COX4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast mitochondria.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-COX4-GFP (COX4 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS306-OsTIR1F74A

    Plasmid

    #198416

    Purpose

    Expresses OsTIR1F74A under the control of ADH1 promoter in budding yeast

    Depositor

    Takumi Kamura

    Article

    Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

    Insert

    OsTIR1F74A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 promoter

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS416-yZ3EV-Z3pr-yEGFP (RB3579)

    Plasmid

    #69100

    Purpose

    URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFP

    Depositor

    David Botstein

    Inserts

    yZ3EV cassette
    Z3 promoter
    yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFA6a-link-yoLSS-mKate2-SpHis5

    Plasmid

    #44848

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoLSS-mKate2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNIA-CEN-FLAG-LINXa4(RVH)-Bre1

    Plasmid

    #116885

    Purpose

    FLAG-tagged LINXa4-Bre1 with RVH hinge-loop mutations

    Depositor

    Brian Kuhlman

    Article

    Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

    Insert

    LINXa4(RVH)-Bre1
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

    Promoter

    ADH1

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGI3EM17

    Plasmid

    #210569

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    SpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
    

    Use

    Luciferase

    Tags

    NanoLuc luciferase

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpH2Bpro

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. JME4599

    Plasmid

    #129660

    Purpose

    NATex_CrisprCas9-yl_RFP: CAS9 vector with Nourseothricin ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge