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pGTy1mhis3-AI or pGTy1his3-AI

Plasmid

#62228

Purpose

A Ty1 element tagged with the retrotransposition indicator gene his3-AI is fused to the GAL1 promoter on a multicopy URA3-based shuttle plasmid.

Depositor

David Garfinkel

Publication

Curcio et al Proc Natl Acad Sci U S A. 1991 Feb 1;88(

Insert

Ty1H3 retrotransposon

Expression

Yeast

Promoter

GAL1

Available since

Feb. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pCR

Plasmid

#60619

Purpose

crRNA expression plasmid on pRS423 backbone

Depositor

Huimin Zhao

Publication

Bao et al ACS Synth Biol. 2014 Sep 19.

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMZ288

Plasmid

#59897

Purpose

Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+

Depositor

Mikel Zaratiegui

Publication

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Expression

Yeast

Mutation

Silent muation of the CspCI site

Available since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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HC078 (3xHA-Atg13)

Plasmid

#59544

Purpose

Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

Depositor

Daniel Klionsky

Publication

Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

Insert

ATG13 (ATG13 Budding Yeast)

Tags

3xHA

Expression

Bacterial and Yeast

Mutation

AvrII site created after start codon

Promoter

ATG13

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
FRP791_insul-(lexA-box)1-PminCYC1-Citrine-TCYC1

Plasmid

#58432

Purpose

Induces Citrine expression from a promoter containing 1 lexA box in yeast cells

Depositor

Joerg Stelling

Publication

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

CitrineA206K

Expression

Yeast

Promoter

insul-(lexA-box)1-PminCYC1

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-G9-5FLAG-hphMX6

Plasmid

#49185

Purpose

genomic G9-5FLAG epitope tagging in S. pombe

Depositor

Eishi Noguchi

Publication

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Tags

G9-5FLAG

Expression

Yeast

Available since

Nov. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSNR52-sgTET

Plasmid

#46923

Purpose

Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TRE elements of pTET07 promoter

Depositor

Stanley Qi, Jonathan Weissman

Publication

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

sgRNA targeting endogenous TRE elements of pTET07 promoter

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pCEV-G3-Km

Plasmid

#46817

Purpose

TEF1 and GAL10 promoter controlled expression cassettes with G418 resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Publication

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and GAL10

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCEV-G2-Ph

Plasmid

#46816

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Publication

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and PGK1

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pRS316-4M5.3

Plasmid

#45831

Depositor

Dane Wittrup

Insert

4M5.3

Tags

FLAG

Expression

Yeast

Promoter

GAL1

Available since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pYES-mtBFP

Plasmid

#45055

Depositor

Benedikt Westermann

Publication

Westermann et al Yeast. 2000 Nov;16(15):1421-7.

Insert

mtBFP

Expression

Yeast

Promoter

GAL

Available since

May 24, 2013

Availability

Academic Institutions and Nonprofits only
pFA6a-link-yomEos2-CaURA3

Plasmid

#44887

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mEos2 with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomEos2

Expression

Yeast

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomKO2-SpHis5

Plasmid

#44845

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKO2 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKO2

Expression

Yeast

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pRMH120

Plasmid

#40593

Purpose

Expresses Gal4BD-CRY2PHR and Gal4AD-CIB1 for light-inducible protein expression

Depositor

Chandra Tucker

Publication

Hughes et al Methods. 2012 Aug 15.

Inserts

CRY2 (CRY2 Mustard Weed)
CIB1 (CIB1 Mustard Weed)

Expression

Yeast

Promoter

ADH1 and TEF

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCP5

Plasmid

#35397

Depositor

Daniel Voytas

Publication

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Type

Empty backbone

Use

Reporter

Expression

Yeast

Promoter

GDP promoter

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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pDS250

Plasmid

#34969

Depositor

Michael Glotzer

Publication

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

V416I

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pDS272

Plasmid

#34975

Depositor

Michael Glotzer

Publication

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2-GFP-LOVpep (MID2 A. sativa, Budding Yeast)

Expression

Yeast

Mutation

T406A, T407A

Promoter

TEF

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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Ube2v1

Plasmid

#31429

Depositor

Rachel Klevit

Publication

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2V1 Human)

Tags

HA

Expression

Yeast

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
RPL25-GFP (94)

Plasmid

#24037

Depositor

Michael Rout

Insert

RPL25 (RPL25 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available since

March 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pFA6a-6xGLY-Myc-HIS3MX6

Plasmid

#20768

Depositor

Mark Hochstrasser

Publication

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available since

March 27, 2009

Availability

Academic Institutions and Nonprofits only

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