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pIYC08

Plasmid

#64743

Purpose

Yeast expression plasmid: acsL641P, ALD6, ADH2, ERG10

Depositor

Jens Nielsen

Publication

Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

Insert

ADH2, ALD6, acsL641P, ERG10

Expression

Yeast

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pJZC519

Plasmid

#62278

Purpose

Yeast dCas9-VP64 expression plasmid

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

dCas9-VP64

Tags

Has a VP64 fusion

Expression

Yeast

Mutation

Has mutations in the RuvC1 and HNH domains to ren…

Promoter

pTdh3

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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gRNA-his-HYB

Plasmid

#64333

Purpose

his3 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Publication

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of his3 deletion gRNA cassette

Expression

Yeast

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
gRNA-leu-HYB

Plasmid

#64332

Purpose

leu2 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Publication

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of leu2 deletion gRNA cassette

Expression

Yeast

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pJZC518

Plasmid

#62277

Purpose

Yeast dCas9 expression plasmid

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

dCas9

Expression

Yeast

Mutation

Has mutations in the RuvC1 and HNH domains to ren…

Promoter

pTdh3

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGTy1mhis3-AI or pGTy1his3-AI

Plasmid

#62228

Purpose

A Ty1 element tagged with the retrotransposition indicator gene his3-AI is fused to the GAL1 promoter on a multicopy URA3-based shuttle plasmid.

Depositor

David Garfinkel

Publication

Curcio et al Proc Natl Acad Sci U S A. 1991 Feb 1;88(

Insert

Ty1H3 retrotransposon

Expression

Yeast

Promoter

GAL1

Available since

Feb. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pMZ288

Plasmid

#59897

Purpose

Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+

Depositor

Mikel Zaratiegui

Publication

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Expression

Yeast

Mutation

Silent muation of the CspCI site

Available since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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HC078 (3xHA-Atg13)

Plasmid

#59544

Purpose

Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

Depositor

Daniel Klionsky

Publication

Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

Insert

ATG13 (ATG13 Budding Yeast)

Tags

3xHA

Expression

Bacterial and Yeast

Mutation

AvrII site created after start codon

Promoter

ATG13

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pFA6a-G9-5FLAG-hphMX6

Plasmid

#49185

Purpose

genomic G9-5FLAG epitope tagging in S. pombe

Depositor

Eishi Noguchi

Publication

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Tags

G9-5FLAG

Expression

Yeast

Available since

Nov. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCEV-G3-Km

Plasmid

#46817

Purpose

TEF1 and GAL10 promoter controlled expression cassettes with G418 resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Publication

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and GAL10

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCEV-G2-Ph

Plasmid

#46816

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Publication

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and PGK1

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pRS316-4M5.3

Plasmid

#45831

Depositor

Dane Wittrup

Insert

4M5.3

Tags

FLAG

Expression

Yeast

Promoter

GAL1

Available since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pYES-mtBFP

Plasmid

#45055

Depositor

Benedikt Westermann

Publication

Westermann et al Yeast. 2000 Nov;16(15):1421-7.

Insert

mtBFP

Expression

Yeast

Promoter

GAL

Available since

May 24, 2013

Availability

Academic Institutions and Nonprofits only
pFA6a-link-yomEos2-CaURA3

Plasmid

#44887

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mEos2 with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomEos2

Expression

Yeast

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomKO2-SpHis5

Plasmid

#44845

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKO2 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKO2

Expression

Yeast

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pRMH120

Plasmid

#40593

Purpose

Expresses Gal4BD-CRY2PHR and Gal4AD-CIB1 for light-inducible protein expression

Depositor

Chandra Tucker

Publication

Hughes et al Methods. 2012 Aug 15.

Inserts

CRY2 (CRY2 Mustard Weed)
CIB1 (CIB1 Mustard Weed)

Expression

Yeast

Promoter

ADH1 and TEF

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCP5

Plasmid

#35397

Depositor

Daniel Voytas

Publication

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Type

Empty backbone

Use

Reporter

Expression

Yeast

Promoter

GDP promoter

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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pDS250

Plasmid

#34969

Depositor

Michael Glotzer

Publication

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

V416I

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pDS272

Plasmid

#34975

Depositor

Michael Glotzer

Publication

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2-GFP-LOVpep (MID2 A. sativa, Budding Yeast)

Expression

Yeast

Mutation

T406A, T407A

Promoter

TEF

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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Ube2v1

Plasmid

#31429

Depositor

Rachel Klevit

Publication

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2V1 Human)

Tags

HA

Expression

Yeast

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only