8,173 results
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Plasmid#62387PurposepFA6a Osm1(M33A)-yoEGFP::LEU2DepositorInsertOSM1-EGFP fusion
TagsEGFPExpressionYeastMutationMethionine 33 mutated to AlaninePromoterAdh1pAvailable SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD127
Plasmid#69175PurposeIntegration of GFP tag at Atg27 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg27-EGFP
TagsEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPMVAu5 (R)
Plasmid#98296PurposeEnhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
ExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICXNHG3
Plasmid#49021PurposeFX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage siteDepositorTypeEmpty backboneTags3C protease site (PreScission site), GFP, and dec…ExpressionYeastPromoterAOX1Available SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymiRFP680-URA3
Plasmid#224102PurposeYeast tagging vector to create a gene fusion with yeast optimized miRFP680 with URA3 selectionDepositorInsertymiRFP680
TagsymiRFP680ExpressionYeastAvailable SinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymiRFP680-Kan
Plasmid#224090PurposeYeast tagging vector to create a gene fusion with yeast optimized miRFP680 with Kan selectionDepositorInsertymiRFP680
TagsymiRFP680ExpressionYeastAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymiRFP680-SpHis5
Plasmid#224095PurposeYeast tagging vector to create a gene fusion with yeast optimized miRFP680 with SpHis5 selectionDepositorInsertymiRFP680
TagsymiRFP680ExpressionYeastAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yemiRFP670-URA3
Plasmid#224100PurposeYeast tagging vector to create a gene fusion with yeast optimized emiRFP670 with URA3 selectionDepositorInsertyemiRFP670
TagsyemiRFP670ExpressionYeastAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAWH-largeT
Plasmid#133895PurposePositive control when used in combination with pBWH-p53. Negative control when used in combination with pBWH-Lam. Expression of Gal4AD-largeT hybrid protein. Homology regions for recombination with pBDepositorInsertLargeT
ExpressionYeastPromoterT7Available SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYC56
Plasmid#63910PurposeRecipient integrative vector to construct mCherry tagged fusionsDepositorTypeEmpty backboneTagsmCherryExpressionYeastAvailable SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-VPS10-msGFPx3
Plasmid#105274PurposePlasmid for pop-in/pop-out replacement of Vps10 with Vps10-msGFPx3.DepositorAvailable SinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
Plasmid#58440PurposeCassette to replace yeast promoters with an inducible promoter containing 2 lexA boxesDepositorInsertPTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
ExpressionYeastPromoterPTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hygMV
Plasmid#73891Purposefission yeast drug selection markerDepositorInserthygMV
ExpressionBacterial and YeastPromoterpombe TEFAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pYM29-BirA*
Plasmid#160291PurposeUsed for C-terminal BirA* tagging of proteins in Saccharomyces cerevisiae; Selection marker: TRP1DepositorInsertBirA*
ExpressionYeastAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Designed VEGF-binding scFv G6des1
Plasmid#110212PurposeYeast Surface Display of G6des1DepositorInsertG6des1
Tagscmyc tagExpressionYeastMutationL A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…Available SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB4787
Plasmid#106147PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4281
Plasmid#98700PurposeA Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX systemDepositorInsertCas9
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4283
Plasmid#98702PurposeA plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG414GAL-ccdB
Plasmid#14143DepositorTypeEmpty backboneUseGateway destinationExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
ZJOM86
Plasmid#133634PurposeIntegration of ANP1-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.DepositorAvailable SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK
Plasmid#64188Purposeharbours marker gene LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV0620
Plasmid#133506PurposeExpression of eGFP tagged PCNA to monitor DNA replicationDepositorInsertpUra4(AfeI)-p(pcn1)-eGFP-pcn1-terminator(nmt1)-natMX
ExpressionYeastAvailable SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pI3-MTU-DO-G418
Plasmid#160023PurposeIntegrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). G418 selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACT2.2gtwy
Plasmid#11346DepositorInsertgateway cassette
TagsGal4 Activation DomainExpressionYeastAvailable SinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pESC-ARG (V5 and HA)
Plasmid#222493PurposeCo-expression of two genes in yeast with V5 and HA tags and ARG1 selectionDepositorTypeEmpty backboneTagsHA and V5ExpressionYeastAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-TYR
Plasmid#222484PurposeCo-expression of two genes in yeast with FLAG and MYC tags and TYR1 selectionDepositorTypeEmpty backboneTagsFLAG and MycExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-MET (V5 and HA)
Plasmid#222490PurposeCo-expression of two genes in yeast with V5 and HA tags and MET15 selectionDepositorTypeEmpty backboneTagsHA and V5ExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-LYS (V5 and HA)
Plasmid#222491PurposeCo-expression of two genes in yeast with V5 and HA tags and LYS2 selectionDepositorTypeEmpty backboneTagsHA and V5ExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-TYR (V5 and HA)
Plasmid#222492PurposeCo-expression of two genes in yeast with V5 and HA tags and TYR1 selectionDepositorTypeEmpty backboneTagsHA and V5ExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-THR (V5 and HA)
Plasmid#222494PurposeCo-expression of two genes in yeast with V5 and HA tags and THR1 selectionDepositorTypeEmpty backboneTagsHA and V5ExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-THR
Plasmid#222479PurposeCo-expression of two genes in yeast with FLAG and MYC tags and THR1 selectionDepositorTypeEmpty backboneTagsFLAG and MycExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-ADE
Plasmid#222480PurposeCo-expression of two genes in yeast with FLAG and MYC tags and ADE2 selectionDepositorTypeEmpty backboneTagsFLAG and MycExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-ARG
Plasmid#222481PurposeCo-expression of two genes in yeast with FLAG and MYC tags and ARG1 selectionDepositorTypeEmpty backboneTagsFLAG and MycExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
ymTq2-yeVenus pDRF1-GW
Plasmid#168076PurposeFRET fusion between ymTq2 and yeVenus for use in yeastDepositorInsertymTq2-yeVenus
ExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PHO5-GFP-Osh1
Plasmid#58835PurposeExpresses GFP-Tagged Osh1 in yeastDepositorAvailable SinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBBK84 pHomL-RPL18Bp-PDR16-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179068PurposeVector containing a yeast integration cassette for RPL18B-driven expression of PDR16-Link-Scarlet(rfp), a marker for the lipid dropletDepositorInsertRPL18Bp-PDR16-Link-Scarlet(rfp)
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK79 pHomL-RPL18Bp-Scarlet(rfp)-Link-YPT7-SSA1t-HomR (AmpR)
Plasmid#179063PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-YPT7, a marker for the vacuole, late endosomeDepositorInsertRPL18Bp-Scarlet(rfp)-Link-YPT7
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG303GPD-ccdB
Plasmid#14134DepositorTypeEmpty backboneUseGateway destinationExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS415_pGal-nCas9(H840A)
Plasmid#79616PurposeExpresses nCas9(H840A) in yeast cellsDepositorInsertSpCas9
ExpressionYeastMutationH840A for nickase Cas9PromoterpGal1Available SinceSept. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJZC533
Plasmid#135435PurposeYeast integrating plasmid for pTEF1-mCherry reporter geneDepositorInsertmCherry
ExpressionYeastMutationcodon optimized for S. cerevisiaePromoterpTEF1Available SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB2196
Plasmid#67549PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked bleMX marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-EGFP
Plasmid#166143PurposeEGFP expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.DepositorInsertEGFP
ExpressionYeastPromoterGAL1Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-mRuby3
Plasmid#166144PurposemRuby3 expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.DepositorInsertmRuby3
ExpressionYeastPromoterGAL1Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
RIB VP64 R1
Plasmid#161493PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting R1 in the RIB1 promoter)
ExpressionYeastPromoterpGAP, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2-5INS_16
Plasmid#102292PurposeYeast Surface Display of 5Ins_16 scFVDepositorInsertInsulin binding scFV
Tags3x(G4S) Linker, G3S Linker, HA, and MycExpressionYeastPromoterGAL1Available SinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
ZJOM157
Plasmid#133640PurposeIntegration of GFP-PEP12 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis).Marker for yeast late endosome.DepositorAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG306-GPD-VMA1 chr I
Plasmid#41896PurposeYeast expression of VMA1.DepositorInsertVMA1 (VMA1 Budding Yeast)
ExpressionYeastMutationContains silent mutation at 342A-G and L87S mutat…PromoterGPDAvailable SinceApril 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
p413TEF1-ATR1(pYL573)
Plasmid#178288PurposeExpresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1DepositorInsertATR1
ExpressionYeastPromoterTEF1Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS305-mAID-LaM2
Plasmid#198413PurposeExpress mAID-Nb(LaM2) under the control of ADH1 promoter in budding yeastDepositorInsertmAID-Nb (LaM2)
ExpressionYeastPromoterADH1 promoterAvailable SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only