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8,265 results

Showing: 1501 - 1550 of 8265 results

  4. pDB4280

    Plasmid
    #98699
    Purpose
    A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system
    Depositor
    Li-Lin Du
    Article
    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    98699-plasmid-map-sequence-id-182830

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  7. p404GAL1

    Plasmid
    #17417
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    GAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    GAL1 promoter is between SacI and XbaI restrictio…
    Available Since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17417-plasmid-map-sequence-id-8592

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  11. p416-GPD-DIPP1-HA

    Plasmid
    #183946
    Purpose
    Expresses human HA-tagged DIPP1 from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    DIPP1 (NUDT3 Human)
    Tags
    HA
    Expression
    Yeast
    Mutation
    WT
    Promoter
    GPD
    Available Since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183946-plasmid-map-sequence-id-368066

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  14. pDRFLIP38

    Plasmid
    #65516
    Purpose
    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Tags
    6His-edCit-attR1 and attR2-edCer-cMyc
    Expression
    Yeast
    Promoter
    pPMA1
    Available Since
    Oct. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    65516-plasmid-map-sequence-id-111426

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  28. pNSU299_GAL2

    Plasmid
    #166088
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GAL2 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    GAL2 (GAL2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166088-plasmid-map-sequence-id-329112

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  29. pCL28-decoder

    Plasmid
    #159450
    Purpose
    Insert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    PlexAtet-LexA-ER-B112-tCyc1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    PlexAtet
    Available Since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159450-plasmid-map-sequence-id-313198

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  30. pCL66-decoder

    Plasmid
    #159453
    Purpose
    Insert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    Pfus1mut-tetR-nls-malE-tCyc1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    Pfus1mut
    Available Since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159453-plasmid-map-sequence-id-313208

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  31. pAV10.KH

    Plasmid
    #63208
    Purpose
    pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    63208-plasmid-map-sequence-id-104909

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  36. pE5c

    Plasmid
    #17465
    Purpose
    modified pENTR vector for C-terminal ECFP tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    ECFP
    Expression
    Bacterial, Insect, Mammalia…
    Available Since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17465-plasmid-map-sequence-id-8633

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  40. pACT2.2gtwy

    Plasmid
    #11346
    Depositor
    Guy Caldwell
    Insert
    gateway cassette
    Tags
    Gal4 Activation Domain
    Expression
    Yeast
    Available Since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only

  42. pCfB6576

    Plasmid
    #106134
    Purpose
    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    106134-plasmid-map-sequence-id-206167

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  43. CUP1-∆ssCPY*-mGFP in pRS315

    Plasmid
    #221158
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Tags
    mGFP
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Available Since
    Nov. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    221158-plasmid-map-sequence-id-450962

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  45. ZJOM22

    Plasmid
    #133629
    Purpose
    Integration of EMC1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast endoplasmic reticulum (ER).
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-EMC1-2GFP (EMC1 Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133629-plasmid-map-sequence-id-265897

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  47. pVG122

    Plasmid
    #211765
    Purpose
    Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:
    Insert
    strongLOV
    Use
    For single-copy integration (if cut with pmei)
    Tags
    SV40 NLS and VP16
    Expression
    Yeast
    Mutation
    compared to EL222, strongLOV contains Glu84Asp mu…
    Available Since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211765-plasmid-map-sequence-id-417945

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Showing: 1501 - 1550 of 8265 results