7,933 results
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Plasmid#19345DepositorInsertnatMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pINER5
Plasmid#185895PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 25, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBEVY-GA
Plasmid#51227Purposebi-directional expression vector for yeast, galactose inducible, ADE2 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterGAL1,10Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
linker-mCitrine-PIF6-NAT
Plasmid#51576PurposeTag protein of interest with PIF6 in Saccharomyces cerevisiaeDepositorInsertPIF6 (PIL2 Mustard Weed)
UseTagslinker and mCitrineExpressionYeastMutation1-100aa of PIF6PromoternoneAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
HSP104-b/Leu(WT)
Plasmid#1156DepositorInsertHSP104 (HSP104 Budding Yeast)
UseTagsGAL1:10 promoterExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGBK CUP1 EGFP
Plasmid#170210PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing EGFPDepositorArticleInsertenhanced green fluorescent protein
UseTagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastMutationPromoterCUP1Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAH237
Plasmid#121146PurposeExpression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeastDepositorInsertsgRNA cassette
humanized Streptococcus pyogenes Cas9
UseCRISPRTagsFlagExpressionYeastMutationPromoterS.pombe rrk1 and nmt41Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYB-Dual(mNG(yeast-opt))
Plasmid#216727PurposeDual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.DepositorInsertmNeonGreen
UseTagsExpressionBacterial and YeastMutationPromoterpGAL1(yeast) and pT7-RNAP(bacteria)Available sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV0751
Plasmid#133485PurposeExpression in fission yeast from promoter pnmt1 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(nmt1)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTIN
Plasmid#162885PurposeOverexpression vector for the fission yeast Schizosaccharomyces pombeDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRY003
Plasmid#81043PurposeHO endonuclease expressionDepositorInsertHO
UseTagsExpressionYeastMutationPromoterendogenousAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJZC545
Plasmid#62313PurposesgRNA with 1x MS2 for yeast cellsDepositorInsertsgRNA + 1x MS2 binding module
UseTagsExpressionYeastMutationPromoterSNR52Available sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEK19
Plasmid#210578PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdBIP1pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastMutationPromoterBdBIP1proAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
M3927 ura3::KanMX3 Disruptor Converter
Plasmid#51682PurposeYeast marker swap plasmid for converting URA3 to KanMX3DepositorInsertKanMX3
UseYeast marker swapTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pI1-MTU-DO-HIS
Plasmid#160142PurposeIntegrative vector for K. marxianus, targeting the LAC4 locus. Histidine selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pI4-MTU-DO-HPH
Plasmid#160216PurposeIntegrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Hygromycin selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDS277
Plasmid#34973PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(V529N mutation).DepositorInsertMid2(SS/TM)-GFP-LOVpep
UseTagsExpressionYeastMutationV529NPromoterAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKL1-222
Plasmid#219409PurposeNuclear expression of Synrophin PDZ clamp proteinDepositorInsertpTEF1-NLS-(Syntrophin-PDZ)x4-tADH1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-Osh1-PH
Plasmid#71372PurposeFor analysis of PH domain localization in yeastDepositorInsertOsh1-PH
UseTagsEGFPExpressionYeastMutationPleckstrin homology (PH) domain; aa267-388PromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSSec7-mGFPx3
Plasmid#25449DepositorInsertSec7 (SEC7 Budding Yeast)
UseTagsmGFPx3ExpressionYeastMutationGFP contains A206K for monomerization.PromoterAvailable sinceMarch 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-ccdB-EGFP
Plasmid#14204DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-SAT1
Plasmid#162465PurposepFA-MNase-CaURA3 was double digested to remove the URA3 auxotrophy marker. The SAT1 marker was amplified from pFA-SAT1 and cloned into the AscI-SacI-digested pFA-MNaseDepositorInsert3FLAG-Mnase
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
RPL25-GFP (94)
Plasmid#24037DepositorInsertRPL25 (RPL25 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceMarch 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR)
Plasmid#178989PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
416Gal-FUS-R521C-YFP
Plasmid#29622DepositorInsertFUS (FUS Human)
UseTagsYFPExpressionYeastMutationR521CPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pABY-c25
Plasmid#201769PurposeExpresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeastDepositorInsertNbALFA
UseTags40aa Linker, mNeonGreenExpressionYeastMutationPromoterCYC1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYes2-TaPCS1
Plasmid#49767PurposeExpresses wheat phytochelatin synthase (TaPCS1) in Saccharomyces CerevisiaeDepositorInsertTaPCS1 (PCS1 Triticum aestivum)
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAV0585
Plasmid#133472PurposeIntegration into fission yeast genome at ade6 locus with hphMX selection cassetteDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
M3938 trp1::ADE2 Disruptor Converter
Plasmid#51673PurposeYeast marker swap plasmid for converting TRP1 to ADE2DepositorInsertADE2 (ADE2 Budding Yeast)
UseYeast marker swapTagsExpressionYeastMutationPromoterADE2Available sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
EZ-L439
Plasmid#169146PurposeEncodes OptoINVRT7 circuitDepositorInsertVP16-EL222
UseTagsSV40 NLSExpressionYeastMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-ccdB-HA
Plasmid#14251DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCfB4778
Plasmid#106125PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-UBQLN2
Plasmid#181708PurposeGalactose inducible expression of UBQLN2DepositorInsertUBQLN2 (UBQLN2 Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS423-TyrSgH
Plasmid#163973PurposeHelper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoterDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJOG417
Plasmid#105341PurposeYeast two hybridDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG418
Plasmid#105342PurposeYeast two hybridDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG306-GPD-empty chr I
Plasmid#41895DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterGPDAvailable sinceApril 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
LD209
Plasmid#69182PurposeIntegration of 2GFP tag at Atg9 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg9-2EGFP
UseTags2xEGFPExpressionYeastMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pE4c
Plasmid#17464Purposemodified pENTR vector for C-terminal GST tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsGSTExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAV0752
Plasmid#133486PurposeExpression in fission yeast from promoter pnmt41 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(nmt41)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB9336 (pgRNA_II-1_NatMX)
Plasmid#161589PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site II-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN0606 (pDEST-DHFR F[3] N-term,HygR)
Plasmid#210488PurposepDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB11 (pDEST-DHFR F[1,2] C-term, NatR)
Plasmid#210485PurposepDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)
Plasmid#210487PurposepDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB12 (pDEST-DHFR F[3] C-term, HygR)
Plasmid#210486PurposepDEST vector to tag gene of interest with DHFR F[3] on the C-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS428
Plasmid#11257DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
YIp ADE2-LEU2
Plasmid#122905PurposeADE2-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
prp8-G736E
Plasmid#108450Purposeprp8-G736E in pRS313 backbone for expression in yeastDepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationchanged G736 to EPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMEL10
Plasmid#107916PurposeURA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPTK020-7-attB
Plasmid#84989PurposeType 7, BxbI recognition siteDepositorInsertBxbI recognition site
UseSynthetic BiologyTagsExpressionYeastMutationBsaI site removed (1711 t to a)PromoterNonAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only