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  2. pFA6a-HaloTag-KanMX6

    Plasmid
    #87029
    Purpose
    Tag S. pombe genes in C terminal with HaloTag
    Depositor
    Julien Berro
    Insert
    HaloTag
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Feb. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87029-plasmid-map-sequence-id-173001

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  4. pCAS9i

    Plasmid
    #141249
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141249-plasmid-map-sequence-id-308874

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  5. ZJOM143

    Plasmid
    #133667
    Purpose
    Integration of VPH1-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-VPH1-mTagBFP2 (VPH1 Budding Yeast)
    Use
    Tags
    mTagBFP2
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    133667-plasmid-map-sequence-id-265896

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  12. PhyB-mCherry-Tom7

    Plasmid
    #66571
    Purpose
    Constitutively express mitochondrial localized PhyB in Saccharomyces cerevisiae
    Depositor
    Orion Weiner
    Article
    Jost et al ACS Synth Biol. 2015 Jun 2.
    Insert
    PhyB (PHYB Mustard Weed)
    Use
    Tags
    Tom7 and mCherry
    Expression
    Yeast
    Mutation
    PhyB portion contains aa's 1-908
    Promoter
    pADH1
    Available since
    Oct. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    66571-plasmid-map-sequence-id-126395

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  17. pJRoC30_Th3.14

    Plasmid
    #188068
    Purpose
    FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.14)
    Depositor
    Sarel Fleishman
    Article
    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173
    Insert
    FuncLib-designed high-redox potential laccase from Trametes hirsuta
    Use
    Tags
    S. cerevisiae evolved α-factor prepro-leader sign…
    Expression
    Yeast
    Mutation
    20 PROSS + 4 FuncLib mutations, described in publ…
    Promoter
    Available since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    188068-plasmid-map-sequence-id-370141

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  20. BB3cN_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104906
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    104906-plasmid-map-sequence-id-210667

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Showing: 621 - 640 of 7821 results