8,432 results
-
Plasmid#129658PurposeURA3ex_CrisprCas9-yl_RFP: CAS9 vector with URA3ex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only
-
ZJOM119
Plasmid#133661PurposeIntegration of NAB2-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.DepositorAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAH237
Plasmid#121146PurposeExpression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeastDepositorInsertsgRNA cassette
humanized Streptococcus pyogenes Cas9
UseCRISPRTagsFlagExpressionYeastPromoterS.pombe rrk1 and nmt41Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJW1664
Plasmid#112038PurposeCEN/ARS plasmid expression of mitochondrial Su9-TagBFP marked with HISDepositorInsertSu9
TagsTagBFPExpressionYeastMutationamino acids 1-76Available sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pV1200
Plasmid#111429PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)DepositorPublicationInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-SEC7-mCherry2Bx6
Plasmid#105267PurposePlasmid for pop-in/pop-out replacement of Sec7 with Sec7-mCherry2Bx6.DepositorAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p404-Grx5-GFP1-10
Plasmid#91952PurposeExpresses GFP1-10 in the mitochondrial matrixDepositorInsertGrx5-GFP1-10
ExpressionYeastPromoterGAPAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHS2
Plasmid#81037PurposeHO endonuclease expressionDepositorInsertHYGMX
ExpressionYeastPromoterTEFAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL
Plasmid#79614PurposeExpresses dCas9-SH3 and PmCDA1-SHL in yeast cellsDepositorPublicationInsertsSpCas9
PmCDA1
TagsSH3 domain and SHLExpressionYeastMutationD10A and H840A for nuclease deficient Cas9PromoterpGal1 and pGal10Available sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMZ376
Plasmid#74213PurposeCombined sgRNA/Cas9 vector with KanMX markerDepositorInsertsCas9
rrk1:sgRNA
UseCRISPRExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRG205MX
Plasmid#64535PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAD
Plasmid#64747PurposeYeast expression plasmid: ACC1 (ser659ala, ser1157ala)DepositorInsertACC1 (ser659ala, ser1157ala)
ExpressionYeastAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJW1513
Plasmid#62383PurposepRS316-pTDH3-Su9-TagBFPDepositorInsertsu9-tagBFP
TagsSu9 presequenceExpressionYeastAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPL5608_TEF1*-Cre_TRP1
Plasmid#60931PurposeConstitutive expression vector for Cre-recombinase containing TRP1 selection marker.DepositorInsertCre
TagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastPromoterMutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT222
Plasmid#40942DepositorInsertGFP-LacI**
TagsGFP(S65T, V163A) and SV40 NLSExpressionYeastMutationLacI**/LacI2G(4 mutations) (see comment below); l…PromoterHis3 promoter fragmentAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRP1641_PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1
Plasmid#58441PurposeCassette to replace yeast promoters with an inducible promoter containing 3 lexA boxesDepositorInsertPTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1
ExpressionYeastPromoterPTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-NLS
Plasmid#51571PurposeConstitutively express nuclear localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsNLS and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
M4755 KanMX::LEU2 Disruptor Converter
Plasmid#51685PurposeYeast marker swap plasmid for converting KanMX3 to LEU2DepositorPublicationAvailable sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
HO-Poly-HO
Plasmid#51661Purposeyeast plasmid for integration of target sequence at HO locusDepositorTypeEmpty backboneUseYeast integrationExpressionYeastAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p413TEF-SirT1
Plasmid#51746Purposeoverexpression of human SirT1 in S. cerevisiae, HIS3 markerDepositorAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by