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pLyGo-Kp-1

Plasmid

#163143

Purpose

pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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HO-pGAL-poly-KanMX4-HO

Plasmid

#51664

Purpose

yeast plasmid for integration of target sequence at HO locus, Kan selection, pGAL inducible promoter

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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GAL 103Q+ProGFPp416

Plasmid

#15583

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt103Q + Pro (HTT Human)

Use

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region present

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pCfB8786

Plasmid

#126920

Purpose

Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pIM701

Plasmid

#74642

Purpose

alias pFA6a-ymTagBFP-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and nourseothricin selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

ymTagBFP

Use

Tags

Expression

Yeast

Mutation

Promoter

none

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoEGFP-SpHis5

Plasmid

#44836

Purpose

Yeast tagging vector to create gene fusion with yeast optimized EGFP with SpHis5 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pAV106-pTDH3-mCherry-10xSumo-6xSIM

Plasmid

#116928

Purpose

Yeast integrative vector for expression of the SUMO-SIM complex

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

10xSUMO-6xSIM

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pDRFLIP38

Plasmid

#65516

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Use

Tags

6His-edCit-attR1 and attR2-edCer-cMyc

Expression

Yeast

Mutation

Promoter

pPMA1

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-mYFP-hphMX6

Plasmid

#105155

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu, Thomas Pollard

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mYFP-hphMX6

Use

Tags

mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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3396 pBM272 Bax

Plasmid

#8770

Depositor

Stanley Korsmeyer

Article

Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

Insert

Bax (Bax Mouse)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only
pWS174

Plasmid

#90961

Purpose

Cas9 gap repair vector - NatR

Depositor

Tom Ellis

Insert

Cas9

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-ymNeongreen-CaURA3

Plasmid

#125703

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeongreen

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJZC522

Plasmid

#62279

Purpose

MCP-VP64 fusion

Depositor

Stanley Qi, Wendell Lim

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

MCP-VP64

Use

Tags

VP64

Expression

Yeast

Mutation

Promoter

pAdh

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-mScarletI-KanMX6

Plasmid

#136690

Purpose

Tagging genes in Cterm with mScarletI in fission yeast

Depositor

Julien Berro

Insert

mScarletI

Use

Tags

mScarletI

Expression

Yeast

Mutation

Promoter

None

Available since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymScarletI

Plasmid

#118452

Purpose

Overexpression of yeast codon-optimized mScarletI

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymScarletI

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAG423GAL-ccdB

Plasmid

#14149

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW yAT1.03

Plasmid

#132781

Purpose

FRET biosensor for ATP measurements in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Sens. 2020 Mar 27;5(3):814-822. doi:

Insert

yAT1.03 ymTq2-tdTomato

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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p404-MTS-mCherry-GFP1-10

Plasmid

#91958

Purpose

Expresses mCherry-GFP1-10 in yeast cell mitochondria

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

MTS-mCherry-GFP1-10

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

June 7, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-mCherry-sfGFP-kanMX (pMaM17)

Plasmid

#173452

Purpose

Contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Khmelinskii et al Nat Biotechnol. 2012 Jun 24;30(7):708-14

Insert

mCherry-sfGFP-ADH1ter

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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p3HR2

Plasmid

#220738

Purpose

Component of yeast three hybrid system

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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