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pHS2

Plasmid

#81037

Purpose

HO endonuclease expression

Depositor

John McCusker

Insert

HYGMX

Expression

Yeast

Promoter

TEF

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL

Plasmid

#79614

Purpose

Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Inserts

SpCas9
PmCDA1

Tags

SH3 domain and SHL

Expression

Yeast

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pGal1 and pGal10

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pMZ376

Plasmid

#74213

Purpose

Combined sgRNA/Cas9 vector with KanMX marker

Depositor

Mikel Zaratiegui

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Expression

Yeast

Mutation

Silent muation of the CspCI site

Promoter

adh1 and rrk1

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pRG205MX

Plasmid

#64535

Purpose

Integrative E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pAD

Plasmid

#64747

Purpose

Yeast expression plasmid: ACC1 (ser659ala, ser1157ala)

Depositor

Jens Nielsen

Article

Shi et al MBio. 2014 May 6;5(3):e01130-14. doi: 10

Insert

ACC1 (ser659ala, ser1157ala)

Expression

Yeast

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1513

Plasmid

#62383

Purpose

pRS316-pTDH3-Su9-TagBFP

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

su9-tagBFP

Tags

Su9 presequence

Expression

Yeast

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pPL5608_TEF1*-Cre_TRP1

Plasmid

#60931

Purpose

Constitutive expression vector for Cre-recombinase containing TRP1 selection marker.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Cre

Tags

Driven from a mutant TEF1 promoter with 0.16x act…

Expression

Yeast

Promoter

Mutant TEF1

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pAT222

Plasmid

#40942

Depositor

Angela Taddei

Article

Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

Insert

GFP-LacI**

Tags

GFP(S65T, V163A) and SV40 NLS

Expression

Yeast

Mutation

LacI**/LacI2G(4 mutations) (see comment below); l…

Promoter

His3 promoter fragment

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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FRP1641_PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Plasmid

#58441

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 3 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Expression

Yeast

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-NLS

Plasmid

#51571

Purpose

Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

NLS and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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M4755 KanMX::LEU2 Disruptor Converter

Plasmid

#51685

Purpose

Yeast marker swap plasmid for converting KanMX3 to LEU2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

LEU2 (LEU2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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HO-Poly-HO

Plasmid

#51661

Purpose

yeast plasmid for integration of target sequence at HO locus

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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p413TEF-SirT1

Plasmid

#51746

Purpose

overexpression of human SirT1 in S. cerevisiae, HIS3 marker

Depositor

Markus Ralser

Article

Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

Insert

SirT1 (SIRT1 Human)

Expression

Yeast

Promoter

TEF1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCEV-G4-Ph

Plasmid

#46820

Purpose

TEF1 and HXT7 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and HXT7

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pAG306-GPD-empty chr I

Plasmid

#41895

Depositor

Dan Gottschling

Article

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Type

Empty backbone

Expression

Yeast

Promoter

GPD

Available Since

April 17, 2013

Availability

Academic Institutions and Nonprofits only

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pTH728-CEN-RLuc/maxCFLuc

Plasmid

#38212

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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426Gal-FUS

Plasmid

#29591

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Expression

Yeast

Available Since

July 1, 2011

Availability

Academic Institutions and Nonprofits only
pDR-GW FLIPglu-30μΔ13V

Plasmid

#27998

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLIPglu-30μΔ13V

Tags

eCFP and eYFP

Expression

Yeast

Mutation

D154A in mglB

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pDR-GW FLIPglu-170nΔ13V

Plasmid

#27999

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLIPglu-170nΔ13V

Tags

eCFP and eYFP

Expression

Yeast

Mutation

WT binding protein (no F16A)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pRS416 Gal G294A YFP

Plasmid

#27448

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Yeast

Mutation

Glycine 294 mutated to Alanine (G294A)

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only