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Plasmid#219848Purposeequimolar expression of mScarlet3 and mTurquoise2 in budding yeastDepositorInsertmScarlet3-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRG706_lexO-SbfI_LexA-TF_LEU2MX
Plasmid#154818PurposeInducible expression of SbfI endonuclease in S. cerevisiaeDepositorInsertsSbfI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-Dcr1
Plasmid#22307DepositorInsertDCR1
Use2micronTagsExpressionYeastMutationPromoterAvailable sinceOct. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
BS-Ura3-GFP-Atg8
Plasmid#69194PurposeSingle copy integration of GFP-Atg8, use auxotrophic marker URA3(Kluyveromyces lactis)DepositorInsertGFP-Atg8
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-nLuc
Plasmid#199755PurposeControl construct of elongation reporter to quantify elongation of YFPDepositorInsertNanoluc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCast
Plasmid#200169PurposeE. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozymeDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWS3865
Plasmid#185742PurposeSub-array backbone 1/4DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYDS2.0_AT118-L
Plasmid#222847PurposeYeast-display plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivityDepositorInsertAT118-L
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pFA6a-GBP-kanMX6
Plasmid#89067Purposegenomic GBP epitope C-terminal tagging in S. pombeDepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsGBPExpressionYeastMutationPromoterAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJH2972
Plasmid#100956PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. URA3MX markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-sGFP-Vrg4
Plasmid#160904PurposeExpresses GFP-Vrg4 in yeast cellsDepositorInsertVRG4
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomCherry-CaURA3
Plasmid#44876PurposeYeast tagging vector to create gene fusion with yeast optimized mCherry with CaUra3 selectionDepositorInsertyomCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fSNAP-Phleomycin
Plasmid#188915PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fSNAP-Phleomycin
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG424GAL-ccdB
Plasmid#14151DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-ABP1-iGFPx6
Plasmid#177099PurposeExpresses Abp1-iGFPx6 in yeast cellsDepositorInsertABP1 (ABP1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUDP002
Plasmid#103872PurposeBroad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)DepositorInsertshph
Spcas9 D147Y P411T
UseCRISPRTagsNLSExpressionYeastMutationD147Y P411TPromoterArxula adeninivorans TEF1 and Ashbya gossypii (Er…Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GBP-mCherry-kanMX6
Plasmid#89068Purposegenomic GBP-mCherry epitope C-terminal tagging in S. pombeDepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsGBP-mCherryExpressionYeastMutationPromoterAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTA-Mob 2.0 Tp
Plasmid#188619PurposeMutated pTA-Mob 2.0 Backbone in TraJ Promoter RegionDepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX-(3×FLAG)-TEV-ProtA
Plasmid#52692PurposeFor C-terminal tagging of ORFs with a (3×FLAG)-TEV-ProtADepositorInsert(3×FLAG)-TEV-ProtA
UseTagsExpressionYeastMutationPromotern/aAvailable sinceApril 30, 2014AvailabilityAcademic Institutions and Nonprofits only