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pCas9
Plasmid#162606PurposePre-express Cas9 in yeastDepositorInsertTef1-Cas9
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFPB5A
Plasmid#83556PurposeUsed to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.DepositorInsertGAL1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterGAL1Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mNeonGreen-HIS3MX6
Plasmid#129100PurposeFor tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreenDepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-Strep-tagII-hphMX4
Plasmid#20758DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionYeastMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRS263
Plasmid#122379PurposeCRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codonDepositorInsertHSP90
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
p404TDH3
Plasmid#15973DepositorInsertTDH3 promoter + polylinker + CYC1 terminator (TDH3 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationTDH3 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pATP416-ÂpluxO5-ÂpphlO6-ÂcrtYBI
Plasmid#165977PurposeExpresses carotenoid biosynthesis gene in response to homoserine lactone and 2,4-diacetylphloroglucinol in yeast expressing PhlTA and LuxTADepositorInsertsXanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)
UseSynthetic BiologyTagsExpressionYeastMutationC1281A, G1698A and C66A, C1359TPromoterSynthetic promoter (pluxO5), Synthetic promoter (…Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet8
Plasmid#107449PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet8
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-ADH700-yeCherry-p150-yeGFP-DHFR
Plasmid#24378DepositorInsertpADH1 yeCherry::p150yeGFP-DHFR
UseTagsExpressionYeastMutation1. Truncated version of ADH700 promoter (SbfI/Spe…PromoterAvailable sinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mTID-HA3-HIS3MX6
Plasmid#227983PurposeTo insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID,HIS3MX6 markerDepositorInsertminiTurboID
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fSNAP-Hygromycin
Plasmid#188914PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fSNAP-Hygromycin
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKate2-SpHis5
Plasmid#44852PurposeYeast tagging vector to create gene fusion with yeast optimized mKate2 with SpHis5 selectionDepositorInsertyomKate2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJW1662
Plasmid#112050PurposeC-terminal tag with - AID*(AtIAA17_71-114)-HA and OsTIR1 expression cassette marked with LEUDepositorInsertsIAA17
TIR1
UseTagsHAExpressionYeastMutationonly amino acids 71-114PromoterADH1 and NoneAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYZ158
Plasmid#98414PurposePombe Expression Vector - nmt1 promoter with Sp.leu1 markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRR-ERbeta-5Z
Plasmid#23062DepositorInsertERbeta (ESR2 Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pIMVAu1
Plasmid#98301PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.DepositorInsertHMG2(-309,-153)-HIS3-TEFM1
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymVenus
Plasmid#118454PurposeOverexpression of yeast codon-optimized mVenusDepositorInsertymVenus
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG
Plasmid#158127PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertmTagBFP
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP3915
Plasmid#113335PurposeBeta-estradiol inducible expression vector; Z4 promoter-CYC1 terminator; artificial transcription factor under ACT1 promoterDepositorInsertArtificial Transcription Factor (McIsaac et al, 2013)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only
