8,400 results
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Plasmid#106472PurposeVector for creating C-terminal GFP fusion proteins in yeast.DepositorTypeEmpty backboneTagsGFPExpressionBacterial and YeastPromoterNoneAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only
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pLH_Scr16
Plasmid#100538PurposeCEN/ARS plasmid with PhyBNT-NLS-CreN and PIF3-NLS-CreC expression cassettes for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.DepositorUseCre/Lox and Synthetic BiologyTagsCdc1 NLS, CreC, CreN, and SV40 NLSExpressionYeastMutationAA 1-621 of PhyB/ Mutation nucleotide 576 C-->APromoterFBA1 and TDH3Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMVS102_P3-GFP-NATMX6
Plasmid#99048PurposeEGFP reporter with six binding sites for the Zif268 DNA binding domainDepositorInsertsMcIsaac 2014 P3 promoter
eGFP
clonNAT resistance
ExpressionYeastMutationGAL1 promoter with 4 GAL4 sites removed and repla…PromoterMcIsaac 2014 P3 promoterAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUD628
Plasmid#103018Purposeexpression of a Cpf1 programming crRNA targetting ADE2 (crADE2-3.S)DepositorAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pANCH2-Nat
Plasmid#87249Purposegene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT)DepositorInsertNat
ExpressionYeastAvailable SinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pH3FS
Plasmid#85780PurposeN-ICE plasmid pH3FSDepositorInsertloxP-HphMX-loxP-3FLAG
UseCre/LoxExpressionYeastAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHS3
Plasmid#81038PurposeHO endonuclease expressionDepositorInsertNATMX - HO endonuclease
ExpressionYeastPromoterTEFAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS415_pGal-nCas9(H840A)
Plasmid#79616PurposeExpresses nCas9(H840A) in yeast cellsDepositorInsertSpCas9
ExpressionYeastMutationH840A for nickase Cas9PromoterpGal1Available SinceSept. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2399
Plasmid#67550PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked amdSYM marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRG235
Plasmid#64533PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSP-G2
Plasmid#64737PurposeYeast expression plasmidDepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJZC603
Plasmid#62317PurposesgRNA with 2x PP7 for yeast cellsDepositorInsertsgRNA + 2x PP7 RNA binding module
ExpressionYeastPromoterSNR52Available SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU-HsAIDSc
Plasmid#60810Purposeto express human AID recoded for yeast codon usageDepositorAvailable SinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-PEX3
Plasmid#51572PurposeConstitutively express peroxisome localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsPEX3 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-Htb2
Plasmid#51569PurposeConstitutively express nuclear localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsHTB2 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable SinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
M3925 trp1::KanMX3 Disruptor Converter
Plasmid#51681PurposeYeast marker swap plasmid for converting Trp1 to KanMX3DepositorInsertKanMX3
UseYeast marker swapExpressionYeastAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
M3929 his3::KanMX3 Disruptor Converter
Plasmid#51679PurposeYeast marker swap plasmid for converting HIS3 to KanMX3DepositorInsertKanMX3
UseYeast marker swapExpressionYeastAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
HO-pMET-poly-KanMX4-HO
Plasmid#51665Purposeyeast plasmid for integration of target sequence at HO locus, Kan selection, pMET inducible promoterDepositorTypeEmpty backboneUseYeast integrationExpressionYeastAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAH5
Plasmid#41829PurposeUsed as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for hygromycin resistance.DepositorTypeEmpty backboneUsePcr templateExpressionBacterial and YeastPromoterT7,TEFAvailable SinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPA-TagRFP-SpHis5
Plasmid#44854PurposeYeast tagging vector to create gene fusion with yeast optimized photoactivatable TagRFP with SpHis5 selectionDepositorInsertyoPA-TagRFP
ExpressionYeastAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only