Skip to main content
Addgene
Showing: 901 - 920 of 7821 results

  4. pFA6a-link-tdTomato-SpHis5

    Plasmid
    #44640
    Purpose
    Yeast tagging vector to create gene fusion with tdTomato with SpHis5 selection
    Depositor
    Kurt Thorn
    Insert
    tdTomato
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    44640-plasmid-map-sequence-id-61108

    Enlarge

  8. ZJOM149

    Plasmid
    #133646
    Purpose
    Integration of ELO3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-ELO3-mCherry (ELO3 Budding Yeast)
    Use
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133646-plasmid-map-sequence-id-267526

    Enlarge

  10. pMZ374

    Plasmid
    #59896
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).
    Depositor
    Mikel Zaratiegui
    Article
    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59896-plasmid-map-sequence-id-313658

    Enlarge

  11. pRMH124-VP16-CIB1

    Plasmid
    #40615
    Purpose
    Expresses VP16-CIB1 for light-inducible protein expression when coupled with GalBD-CRY2PHR or GalBD-CRY2
    Depositor
    Chandra Tucker
    Article
    Hughes et al Methods. 2012 Aug 15.
    Insert
    VP16-CIB1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    Oct. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    40615-plasmid-map-sequence-id-52745

    Enlarge

  16. pJH2971

    Plasmid
    #100955
    Purpose
    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX marker
    Depositor
    James Haber
    Article
    Ranjith et al Protocol Exchange (2017)
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    100955-plasmid-map-sequence-id-193248

    Enlarge

  18. pRS316-RGR-GFP

    Plasmid
    #51056
    Purpose
    Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.
    Depositor
    Yunde Zhao
    Article
    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1
    Insert
    RGR-GFP
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Yeast ADH1 promoter
    Available since
    Feb. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51056-plasmid-map-sequence-id-77326

    Enlarge

Showing: 901 - 920 of 7821 results