8,173 results
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Plasmid#131769PurposePADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sitesDepositorInsertFUSN_Fusion Red_Cry2WT
UseIntegration into δ-sitesExpressionYeastPromoterPADH1Available SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17
Plasmid#121386PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastPromoterADH1 promoterAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-T7-HIS3MX6
Plasmid#20759DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable SinceMarch 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAG306GPD-ccdB-HA
Plasmid#14236DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
GTH-r
Plasmid#81106PurposeGene Tagging vector HIS/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInsertmCherry
UseCre/LoxTagsmCherryExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG306GAL-ccdB-HA
Plasmid#14235DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pK3F-GAL1
Plasmid#85772PurposeN-ICE plasmid pK3F-GAL1DepositorInsertloxP-KanMX-loxP-GAL1p-3FLAG
UseCre/LoxExpressionYeastAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
M4757 KanMX::TRP1 Disruptor Converter
Plasmid#51687PurposeYeast marker swap plasmid for converting KanMX3 to TRP1DepositorInsertTRP1 (TRP1 Budding Yeast)
ExpressionYeastAvailable SinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
PP324
Plasmid#78982Purpose246B. Promoter: GAP6, minimal Promoter: AOX1. Figure 5. Expresses rHGH and IFNa2b.DepositorInsertsrHGH
IFNa2b
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW FLIPglu-2µΔ13V
Plasmid#28000DepositorInsertFLIPglu-2µΔ13V
TagseCFP and eYFPExpressionYeastMutationH152AAvailable SinceJune 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDIV489
Plasmid#177697PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRNR2p (Debaryomyces hansenii ) and TDH3p (Candida…Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK59-ATG18-GFP
Plasmid#42529DepositorAvailable SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMJB1
Plasmid#160429PurposeYeast vector for expressing mCherry tagged genes under the GAL1 promoterDepositorTypeEmpty backboneTagsmCherryExpressionYeastPromoterGAL1Available SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
E2-Crimson-FKBP(L,V)
Plasmid#85000PurposeExpresses E2-Crimson-FKBP(L,V) in yeastDepositorInsertE2-Crimson-FKBP(LV)
ExpressionYeastAvailable SinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEK20
Plasmid#210582PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdGAPDHpro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastPromoterBdGAPDHproAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS305-mAID-LaM4
Plasmid#198414PurposeExpress mAID-Nb(LaM4) under the control of ADH1 promoter in budding yeastDepositorInsertmAID-Nb (LaM4)
ExpressionYeastPromoterADH1 promoterAvailable SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS305-mAID-LaM8
Plasmid#198415PurposeExpresesses mAID-Nb(LaM8) under the control of ADH1 promoter in budding yeastDepositorInsertmAID-Nb (LaM8)
ExpressionYeastPromoterADH1 promoterAvailable SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
MtPT4-eGFP
Plasmid#117095Purposeprotein expression in yeastDepositorInsertMtPT4-eGFP
TagsHisExpressionYeastPromoterAOXAvailable SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
PP93
Plasmid#78990PurposeSupplemental Figure 4. No Promoter.DepositorInsertGFP
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDH18
Plasmid#83803PurposeYFP-CFP positive FRET control; his selectionDepositorInsertYFP-CFP
ExpressionYeastAvailable SinceOct. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-mYFP
Plasmid#105144PurposeYeast gene targetingDepositorInsertkanMX6-P81nmt1-mYFP
Tags81nmt1-mYFPExpressionBacterial and YeastMutationA206KPromoter81nmt1Available SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
LHP938 - Gal1-HA-Ub
Plasmid#32175DepositorAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-iGFP-TLG2
Plasmid#105262PurposePlasmid for pop-in/pop-out replacement of Tlg2 with iGFP-Tlg2.DepositorAvailable SinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
LD211
Plasmid#69183PurposeIntegration of 2GFP tag at Atg11 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg11-2EGFP
Tags2xEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-ARF1-msGFP
Plasmid#105255PurposePlasmid for pop-in/pop-out replacement of Arf1 with Arf1-msGFP.DepositorAvailable SinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTY24
Plasmid#83794PurposeUseful for tagging yeast proteins with C-terminal DsRed tag; G418 selectionDepositorInsertDsRed
TagsDsRedExpressionYeastAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomApple-CaURA3
Plasmid#44879PurposeYeast tagging vector to create gene fusion with yeast optimized mApple with CaUra3 selectionDepositorInsertyomApple
ExpressionYeastAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Aga1p-FAPB2.3.6
Plasmid#158147PurposeYeast display reporter for non-display yeast strains (wild-type construct)DepositorInsertAga1p-FAPB2.3.6
ExpressionYeastPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K5
Plasmid#63210PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K4
Plasmid#63212PurposepCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJZC523
Plasmid#62320PurposesgRNA for yeast cellsDepositorInsertsgRNA
ExpressionYeastPromoterSNR52Available SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJT42_GalL_BE-PAPAPA
Plasmid#145035PurposeExpresses BE-PAPAPA in yeast cellsDepositorInsertBE-PAPAP
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MtPT4
Plasmid#117096Purposeprotein expression in yeastDepositorInsertMtPT4
TagsHisExpressionYeastPromoterAOXAvailable SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG414GAL-ccdB-EGFP
Plasmid#14191DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
423Gal-FUS-YFP
Plasmid#29615DepositorAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-G9-5FLAG-kanMX6
Plasmid#49181Purposegenomic G9-5FLAG epitope tagging in S. pombeDepositorTypeEmpty backboneTagsG9-5FLAGExpressionYeastAvailable SinceNov. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB9337 (pgRNA_IV-1_NatMX)
Plasmid#161590PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free GFP-NES
Plasmid#182491PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
GFP-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10 and GAL7Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-Sec7-mScarlet2Ix6
Plasmid#140151PurposePlasmid for pop-in/pop-out replacement of Sec7 with Sec7-mScarlet2Ix6DepositorInsertSEC7
ExpressionYeastAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pILGFPU0 (R)
Plasmid#83562PurposeExpress yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoterDepositorInsertTEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST
ExpressionYeastPromoterTEF1Available SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBM5218
Plasmid#18796DepositorInsertTy5-HIS3AI
ExpressionYeastAvailable SinceJuly 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPICZc-Ub-R-HsACTB*-thymosinB-8His
Plasmid#111149PurposeExpresses arginylated human beta-actin fused with thymosin beta and a His tag.DepositorInsertACTB (ACTB Human)
UsePichia pastoris integration plasmidTagsThymosin beta and a His-tagExpressionYeastMutationN-terminal arginylationPromoterAOX1Available SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG413GPD-ccdB-HA
Plasmid#14238DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastAvailable SinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-3xFLAG-LINKER
Plasmid#52689PurposeSame as 41022, with additional linkerDepositorInserttetO7-Pcyc1-3xFLAG+LINKER
UsePromoter replacementExpressionYeastAvailable SinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pBridge-tyrosinase.σ1A
Plasmid#197381PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-1 σ1A
TagsGAL4-DNA binding domain fragment, HA tag, and SV4…ExpressionYeastMutationContains an extra 42 nucleotides encoding 14 resi…PromoterADH1 and MET25Available SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG424GPD-ccdB-HA
Plasmid#14248DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastAvailable SinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-aC-KHC-BZ
Plasmid#160427PurposeExpression of c-luciferase from sp. Kornikeria hastingsi carriebowae (Belize)DepositorInsertluciferase
UseLuciferaseTags6x Histidine tag, Yeast alpha secretion factor, c…ExpressionYeastPromoterAOXAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAN101
Plasmid#131123PurposePlasmid for overexpression of prion domains to induce prionsDepositorTypeEmpty backboneTagsmKate2ExpressionYeastAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB2227
Plasmid#67536PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only