8,265 results
-
Plasmid#185890PurposeIn vivo gene amplification (HamAmp) of lycopene synthetic module at RPL25 locusDepositorInsertsee comments
ExpressionYeastMutationERG20 F96C; Xd.CRtYB E83KAvailable SinceSept. 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIT6EG7ml
Plasmid#185886PurposeIn vivo gene amplification (HamAmp) of limonene synthetic module at RPL25 locus (14 copy).DepositorInsertsee comments
ExpressionYeastMutationERG20 F96W N127WAvailable SinceSept. 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDRF1-GW mScarletI-T2A-mTurquoise2
Plasmid#118430PurposeProduces equimolar expression of mScarletI and mTq2DepositorInsertmScarletI-T2A-mTurquoise2
ExpressionYeastAvailable SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBJ1807 pRS415-COF1RFP
Plasmid#37102DepositorInsertCOF1-RFP
TagsRFPExpressionYeastMutationSingle RFP internal insertion at Cof147T48D with …Available SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pK3FS-CYC1
Plasmid#85779PurposeN-ICE plasmid pK3FS-CYC1DepositorInsertloxP-KanMX-loxP-CYC1p-3FLAG
UseCre/LoxExpressionYeastAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHES609 [prNOp7-PBAC]
Plasmid#103017PurposeA S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.DepositorInsertbPAC
ExpressionYeastAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFS119
Plasmid#12379DepositorInsertade6, thymidine kinase
ExpressionYeastAvailable SinceSept. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJY3948
Plasmid#113338Purpose6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expressionDepositorInsert2-pyrone synthase, codon optimized for Y. lipolytica
Tags6XHisExpressionYeastMutationPlease see depositor commentsAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDBD-M
Plasmid#50572PurposeDouble Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571DepositorInsertDHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
ExpressionYeastPromoterGal1Available SinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS34
Plasmid#83796PurposeUseful for tagging yeast proteins with C-terminal mCherry tag; G418 selectionDepositorInsertmCherry
TagsmCherryExpressionYeastAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-APL2-iGFPx6
Plasmid#105251PurposePlasmid for pop-in/pop-out replacement of Apl2 with Apl2-iGFPx6.DepositorAvailable SinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pL90-Nat-2xHO
Plasmid#139069PurposeCRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selectionDepositorInsertTwo guide RNAs targeting the HO endonuclease
ExpressionBacterial and YeastPromoterTDH3Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJAS1052
Plasmid#158598PurposeVioA, VioBDepositorInsertsVioA
VioB
ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
LD113
Plasmid#69166PurposeIntegration of GFP tag at Atg13 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg13-EGFP
TagsEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
GTT-g
Plasmid#81109PurposeGene Tagging vector TRP/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)DepositorInsertsfGFP
UseCre/LoxTagssfGFPExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV11K
Plasmid#63184PurposepRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal TDP43 Q331K
Plasmid#27461DepositorAvailable SinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJY4092
Plasmid#113334PurposeVector for Hermes transposition; Hermes TIR-flanked AvrII cut site; recoded transposase driven by TEF1 promoterDepositorInsertcodon-optimized Hermes transposase
ExpressionYeastAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FHyTkAX
Plasmid#73901Purposefission yeast drug counter-selectable selection markerDepositorInserthyg-TK
ExpressionYeastAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
p316CUP1-3SGFP.SG
Plasmid#1207DepositorAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-nLuc
Plasmid#199756PurposeElongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stallingDepositorInsertsynYFP[TTG/AGA]-nLuc
ExpressionYeastPromoterTetO7Available SinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfB2899(X-2 MarkerFree)
Plasmid#73271PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pL
Plasmid#64177Purposeharbours marker gene LEU2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW FLIPglu-2µΔ13V
Plasmid#28000DepositorInsertFLIPglu-2µΔ13V
TagseCFP and eYFPExpressionYeastMutationH152AAvailable SinceJune 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
POM43-mCherry
Plasmid#69857PurposeN-terminal tagging with mCherry using LEU2 as a selection marker in S. cerevisiae.DepositorInsertmCherry minus start and stop codon
ExpressionYeastAvailable SinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIYC05
Plasmid#64742PurposeYeast expression plasmid: acsL641P, ALD6DepositorInsertacsL641P, ALD6
ExpressionYeastAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP42
Plasmid#65518PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-Cit-attR1 and attR2-mCer-cMycExpressionYeastPromoterpPMA1Available SinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP35
Plasmid#65513PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-TFPt9-cMycExpressionYeastPromoterpPMA1Available SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP32
Plasmid#65511PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-t7CFPt9-cMycExpressionYeastPromoterpPMA1Available SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
E2-Crimson-FKBP(M)
Plasmid#85002PurposeExpresses E2-Crimson-FKBP(M)DepositorInsertE2-Crimson-FKBP(M)
ExpressionYeastAvailable SinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA CRU HISMX
Plasmid#131164PurposePCR template for genomic C-terminal Cub-R-URA3 tag, HISMX complements the histidine auxotrophy of S. cerevisiae his mutantDepositorTypeEmpty backboneTagsCRUExpressionYeastAvailable SinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA NuiHA-loxP-nat-CUP1p
Plasmid#131170PurposePCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to NourseothricinDepositorTypeEmpty backboneTagsNui-HAExpressionYeastAvailable SinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
GTH-ir
Plasmid#81107PurposeGene Tagging vector HIS/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInserttdiRFP
UseCre/LoxTagstdiRFPExpressionYeastAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-ANP1-msGFP2
Plasmid#177100PurposeExpresses Anp1-msGFP2 in yeast cellsDepositorInsertANP1 (ANP1 Budding Yeast)
ExpressionYeastAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GYB
Plasmid#160927PurposeDual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct)DepositorInsertsBFP
sfGFP
ExpressionYeastPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNS3
Plasmid#131770PurposePADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sitesDepositorInsertPADH1_FUSN_Fusion Red_Cry2Olig_TACT1
UseIntegration into δ-sitesExpressionYeastMutationE490G to make Cry2 into Cry2OligPromoterPADH1Available SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-APL5-mCherry2Bx6
Plasmid#105253PurposePlasmid for pop-in/pop-out replacement of Apl5 with Apl5-mCherry2Bx6.DepositorAvailable SinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoLSS-mKate2-CaURA3
Plasmid#44886PurposeYeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with CaUra3 selectionDepositorInsertyoLSS-mKate2
ExpressionYeastAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG424GAL-EGFP-ccdB
Plasmid#14319DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKT0103
Plasmid#8718DepositorInsertyEVenus
TagsCodon optimized linkerExpressionYeastMutationyEGFP with F46L, S65G, V68L, S72A, M153T, V163A, …Available SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYC121
Plasmid#63917PurposeRecipient overexpressing vector to construct MYC tagged fusionsDepositorTypeEmpty backboneTagsMYCExpressionYeastPromoterTEF1 (Saccharomyces cerevisiae)Available SinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
LHP938 - Gal1-HA-Ub
Plasmid#32175DepositorAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAG416GALL-ccdB-6stop
Plasmid#203511PurposeGateway-compatible destination vector for yeast expression with GALL promoter/6stop mutation/URA3 markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-3x-uC-ccdB-6stop
Plasmid#203514PurposeGateway-compatible destination vector for yeast expression with GAL1 promoter (3x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-ccdB-6stop
Plasmid#203512PurposeGateway-compatible destination vector for yeast expression with GAL10 promoter/6stop mutation/URA3 markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-1x-ccdB-6stop
Plasmid#203519PurposeGateway-compatible destination vector for yeast expression with GAL1 promoter (1x GAL4 binding sites)/6stop mutation/URA3 markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-2x-uA-ccdB-6stop
Plasmid#203517PurposeGateway-compatible destination vector for yeast expression with GAL1 promoter (2x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-DENV_NS3-GS-NS2B
Plasmid#201940PurposeExpresses DENV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 markerDepositorAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GALL-HIV-1_PR
Plasmid#203493PurposeExpresses HIV-1 protease from a GALL promoter with a URA3 markerDepositorInsertHIV-1 protease
ExpressionYeastPromoterGALLAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-WNV_NS2B-GS-NS3
Plasmid#203475PurposeExpresses WNV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 markerDepositorAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only