8,350 results
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Plasmid#128524PurposeExpresses mutant Ber e 1 in PichiaDepositorInsertmutant Ber e 1
ExpressionYeastPromoterAOX1Available SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
APEX2 VLP
Plasmid#182211PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and APEX2 tagged with VP2C (GAL10 promoter).DepositorInsertsVP2C-APEX2
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic Biology; Tem imagingExpressionYeastPromoterGAL1 and GAL10Available SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pV2A-T
Plasmid#153022PurposeSingle-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoterDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP43
Plasmid#65519PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-edAFPt9-attR1 and attR2-edCer-cMycExpressionYeastPromoterpPMA1Available SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
prs316 CUP-GFP
Plasmid#128052PurposeYeast expression vector for N terminal GFP fusionsDepositorTypeEmpty backboneTagsGFPExpressionYeastAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP39
Plasmid#65517PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMycExpressionYeastPromoterpPMA1Available SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC48
Plasmid#63905PurposeRecipient integrative vector to construct HA tagged fusionsDepositorTypeEmpty backboneTagsHAExpressionYeastAvailable SinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
ZJOM2
Plasmid#133633PurposeIntegration of VRG4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.DepositorAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB2803
Plasmid#63646PurposepCfB2803 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoternoneAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV0758
Plasmid#133511PurposeExpression of 3GFP tagged RasAct probe to monitor active Ras1DepositorInsertpLys3(BstZ17I)-p(pak1)-RasAct(3xbyr2RBD)-3GFP-kanMX-terminator(ScADH1)
ExpressionYeastAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0783
Plasmid#133515PurposeExpression of mCherry tagged LifeAct probe to monitor actin cytoskeletonDepositorInsertpAde6(PmeI)-p(act1)-LifeAct-mCherry-terminator(ScADH1)-bsdMX
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAL-SKI1-NAT
Plasmid#160382PurposeOverxpression of SKI1/XRN1 in yeastDepositorInsertSKI1
ExpressionYeastPromoterGALAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Vrg4-V5-APEX2-URA
Plasmid#207071PurposeExpressing Vrg4-V5-APEX2. Marker for late Golgi/early endosomes.DepositorInsertVrg4
TagsV5-APEX2ExpressionYeastPromoterpVRG4Available SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-4V5-APEX2-URA
Plasmid#207056PurposeFor ORF tagging with 4V5-APEX2DepositorInsert4xV5-APEX2
Tags4xV5-APEX2ExpressionYeastAvailable SinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pROS15
Plasmid#107929Purposenat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG425GPD-Cerulean-ccdB
Plasmid#14418DepositorTypeEmpty backboneUseGateway CloningTagsCeruleanExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMOD8A-RGR-r10
Plasmid#74370PurposeThis plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorArticleInsertRGR-r10
UseCRISPR and Synthetic BiologyExpressionYeastPromoterADH1Available SinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMM0553
Plasmid#128989PurposeLEU2 integration vector of pZF(3BS)-mRUBY2DepositorInsertLeu2 5' homology-pZiF promoter-mRuby2-tADH1-Con1-LEU2-Leu2 3' homology
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRP1483
Plasmid#64511PurposeExpresses destabilized Ametrine in yeast.DepositorInsertAmetrine-ADH1-tail
ExpressionYeastPromoterTDH3Available SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-SYS1-msGFPx3
Plasmid#177112PurposeExpresses Sys1-msGFP3 in yeast cellsDepositorInsertSYS1 (SYS1 Budding Yeast)
ExpressionYeastAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACT4-DM
Plasmid#111233Purposeyeast expression vectorDepositorInsertccdB-cassette
ExpressionYeastAvailable SinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc Dam_f_GFPoNLS
Plasmid#85820PurposeiDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc Dam_f_GFPoNLS
TagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A.Available SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-AP
Plasmid#61830PurposeEncodes SNAP-AP fusion for tagging endogenous target for SNAP-SiMPullDepositorInsertSNAP-AP
UsePcr templateExpressionBacterial and YeastAvailable SinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFTK043
Plasmid#171315PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInserteGFP fluorescent reporter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB6578
Plasmid#106130PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPMVAugw
Plasmid#98300PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.DepositorInsertTEFM1EfmvaE>TEBS1
ExpressionYeastAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIp128-P30-His-pol30(K164R)
Plasmid#99544PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; Leu2 markerDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046348
Plasmid#149108PurposeYeast Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
TagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDH3
Plasmid#83789PurposeUseful for tagging yeast proteins with C-terminal CFP tag; G418 selectionDepositorInsertCFP
TagsCFPExpressionYeastMutationF64L, Y66W, N146I, M153T, V163A, N164H mutations …Available SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB6677
Plasmid#106155PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6632, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYC61
Plasmid#63925PurposeRecipient replicative vector to construct CFP tagged fusionsDepositorTypeEmpty backboneTagsCFPExpressionYeastAvailable SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC55
Plasmid#63922PurposeRecipient replicative vector to construct YFP tagged fusionsDepositorTypeEmpty backboneTagsYFPExpressionYeastAvailable SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC42
Plasmid#63920PurposeRecipient replicative vector to construct mCherry tagged fusionsDepositorTypeEmpty backboneTagsmCherryExpressionYeastAvailable SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICE LYS2
Plasmid#122879PurposeLYS2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceMay 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFS119
Plasmid#12379DepositorInsertade6, thymidine kinase
ExpressionYeastAvailable SinceSept. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pILGFP97
Plasmid#83551PurposeUsed to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.DepositorInsertCYC1 promoter-yEGFP
ExpressionYeastPromoterCYC1Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV0714
Plasmid#133479PurposeExpression in fission yeast from promoter pact1, integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(act1)-sfGFP-terminator(ScCYC1)
ExpressionYeastAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK3FS-CYC1
Plasmid#85779PurposeN-ICE plasmid pK3FS-CYC1DepositorInsertloxP-KanMX-loxP-CYC1p-3FLAG
UseCre/LoxExpressionYeastAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pDS1209
Plasmid#177340PurposeExpresses Aequorin in Candida albicans using the URA3 auxotrophic marker URA3DepositorInsertAequorin
ExpressionYeastPromoterACT1Available SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW FLIPglu-2µΔ13V
Plasmid#28000DepositorInsertFLIPglu-2µΔ13V
TagseCFP and eYFPExpressionYeastMutationH152AAvailable SinceJune 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW mScarletI-T2A-mTurquoise2
Plasmid#118430PurposeProduces equimolar expression of mScarletI and mTq2DepositorInsertmScarletI-T2A-mTurquoise2
ExpressionYeastAvailable SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal TDP43 Q331K
Plasmid#27461DepositorAvailable SinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2-Cterminal6xHis
Plasmid#60386PurposeExpression of yeast beta tubulin (TUB2)-Cterminal 6xHis using a GAL promoterDepositorInsertTUB2
Tags6xHISExpressionYeastPromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICE TRP1
Plasmid#122881PurposeTRP1 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceOct. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FHyTkAX
Plasmid#73901Purposefission yeast drug counter-selectable selection markerDepositorInserthyg-TK
ExpressionYeastAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTT-g
Plasmid#81109PurposeGene Tagging vector TRP/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)DepositorInsertsfGFP
UseCre/LoxTagssfGFPExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS34
Plasmid#83796PurposeUseful for tagging yeast proteins with C-terminal mCherry tag; G418 selectionDepositorInsertmCherry
TagsmCherryExpressionYeastAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHES609 [prNOp7-PBAC]
Plasmid#103017PurposeA S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.DepositorInsertbPAC
ExpressionYeastAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only