8,025 results

1. pROS13

   Plasmid

   #107927

   Purpose

   kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y, gRNA-ADE2.Y
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p416PGK1-OsCYP711A2(pYL770)

   Plasmid

   #178301

   Purpose

   Expresses OsCYP711A2 from O. sativa in Saccharomyces cerevisiae. Centromeric URA, PPGK1-OsCYP711A2-Tpho5

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Insert

   OsCYP711A2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PGK1

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAV0771

   Plasmid

   #133513

   Purpose

   Expression of mTagBFP2 tagged CHD domain to monitor actin cytoskeleton

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(act1)-3mTagBFP2-CHDRng2-terminator(ScCYC1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRS415-Cas9-VPR

   Plasmid

   #163971

   Purpose

   Trifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeast

   Depositor

   Jiazhang Lian

   Article

   Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

   Insert

   Cas9-VPR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAV0612

   Plasmid

   #133505

   Purpose

   Expression of 3xmTagBFP2 targeted to the plasma membrane with an amphipathic helix of RitC

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(tdh1)-3mTagBFP2-RitC-terminator(ScADH1)-bsdMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p41Nat MagicFluor

   Plasmid

   #58563

   Purpose

   p41Nat carrying both mating type markers buffered by the Cyc1 terminator

   Depositor

   Leonid Kruglyak

   Insert

   Ste2p-Citrine, Cyc1 term, Ste3p-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pICXC3H

   Plasmid

   #49022

   Purpose

   FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site and 10x His tag

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site) and decaHis (…

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGM-ENO2-YFP-mCherry

   Plasmid

   #180569

   Purpose

   Dual fluorescent reporter for 5' UTR activity in yeast YFP/mCherry

   Depositor

   Joel McManus

   Article

   May et al Elife. 2023 May 25;12:e69611. doi: 10.75

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFA6a-hphMX6-P81nmt1-3FLAG

   Plasmid

   #19350

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Aug 26. ():.

   Insert

   hphMX6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCfB2374

    Plasmid

    #67534

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae XI-1 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. YIplac211-VPS8-iYFPx6

    Plasmid

    #105272

    Purpose

    Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-iYFPx6.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Vps8 portion (VPS8 Budding Yeast)
    

    Use

    Tags

    iYFPx6

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHES834

    Plasmid

    #87947

    Purpose

    Expresses mKate2 under control of GAL1 promoter.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    pGAL1-mKate2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGal1

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p415 Gcs1

    Plasmid

    #112646

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Gcs1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Met25

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYEXC3GH

    Plasmid

    #49027

    Purpose

    FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAH6

    Plasmid

    #41830

    Purpose

    Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for ClonNAT resistance

    Depositor

    Melissa Moore

    Article

    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

    Type

    Empty backbone

    Use

    Pcr template

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    T7,TEF

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCfB2804

    Plasmid

    #63644

    Purpose

    pCfB2804 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-link-yoClover-SpHis5

    Plasmid

    #44857

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized Clover with SpHis5 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoClover
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHCas9-Nours

    Plasmid

    #107733

    Purpose

    Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

    Depositor

    Sheng Yang

    Article

    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Human optimized

    Promoter

    TEF1

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJZC595

    Plasmid

    #62322

    Purpose

    Expresses MCP-VP64 and dCas9 in Yeast cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    MCP-VP64
    dCas9
    

    Use

    Tags

    VP64

    Expression

    Yeast

    Mutation

    Has nuclease-inactivating mutations in the RuvC1 …

    Promoter

    Tdh3 and pAdh

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFA6a-PATagRFP-KanMX6

    Plasmid

    #87027

    Purpose

    Tag S. pombe genes in C terminal with PATagRFP

    Depositor

    Julien Berro

    Insert

    PATagRFP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAG306GPD-EGFP-ccdB

    Plasmid

    #14308

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJAS1668

    Plasmid

    #158593

    Purpose

    VioE-ePTS1 CEN6/ARS4

    Depositor

    John Dueber

    Article

    Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

    Insert

    VioE-ePTS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMC6-TEFkFLP(LYS2)

    Plasmid

    #49458

    Purpose

    overespression of Flp step-arrest mutant in yeast, LYS2 selection

    Depositor

    Marc Gartenberg

    Insert

    Flp(H305L) (flp Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H305L, ‘step-arrest’ mutant,

    Promoter

    TEF1

    Available since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFA6a-KanMX6-Purg1-3FLAG

    Plasmid

    #19354

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Aug 26. ():.

    Insert

    urg1 promoter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDRF1-GW AKAR3-EV-NR

    Plasmid

    #182534

    Purpose

    Expresses the non responsive PKA FRET sensor AKAR3-EV (T506A) in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al FEMS Yeast Res. 2023 Jan 4;23:foad029. d

    Insert

    AKAR3-EV-NR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T506A mutation

    Promoter

    PMA1

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pWS1837

    Plasmid

    #123058

    Purpose

    _-factor pheromone

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    _-factor [3-4a]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pESC-NAT-Ago-Dcr

    Plasmid

    #160381

    Purpose

    Overexpression of Ago-Dcr fusion protein in yeast

    Depositor

    John McCusker

    Insert

    Ago- Dcr
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGBW-m4046299

    Plasmid

    #153878

    Purpose

    Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pAGT572_Nemo

    Plasmid

    #153487

    Purpose

    Level 1 episomal expression plasmid for S. cerevisiae

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPAP002

    Plasmid

    #153489

    Purpose

    Level 1 episomal expression plasmid for P. pastoris

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDIV494

    Plasmid

    #177701

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RNR2p (Debaryomyces hansenii ), SNR52p (Candida s…

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHES854

    Plasmid

    #89197

    Purpose

    Expresses mKate2 under control of TDH3 constitutive promoter.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    pTDH3-mKate2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAG414GPD-ccdB-HA

    Plasmid

    #14240

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pNRB143

    Plasmid

    #36452

    Depositor

    Douglas Bishop

    Article

    Bishop et al Genes Cells. 1999 Aug;4(8):425-44.

    Insert

    RAD54 (RAD54 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    T7

    Available since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

35. pFA0055

    Plasmid

    #131774

    Purpose

    Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.

    Depositor

    Frank Albert

    Article

    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

    Insert

    20mer CASS5a guide (gCASS5a) and 5' sgRNA
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 1000.pCCLsin.cPPT.hPGK.Myt1L.MIRT124_x4 Xma_lost.WPRE

    Plasmid

    #67293

    Purpose

    Induce human iN conversion from fibroblast

    Depositor

    Malin Parmar

    Article

    Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

    Insert

    Myt1L (Myt1l Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    PGK

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. BB3aZ_14*

    Plasmid

    #98542

    Purpose

    empty BB3 for insertion and overexpression of 1 gene in P. pastoris

    Depositor

    Michael Sauer, Brigitte Gasser, Diethard Mattanovich

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJW1667

    Plasmid

    #112041

    Purpose

    CEN/ARS plasmid for GAL-inducible ERG5-V5-TEV-EGFP expression marked with URA

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    ERG5
    

    Use

    Tags

    V5-TEV-EGFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCT-L7.5.1

    Plasmid

    #42900

    Depositor

    Dane Wittrup

    Article

    Hackel et al J Mol Biol. 2008 Sep 19;381(5):1238-52.

    Insert

    L7.5.1
    

    Use

    Tags

    HA and c-myc

    Expression

    Yeast

    Mutation

    Promoter

    Gal1-10

    Available since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. cyterm-ymScarletI pDRF1-GW

    Plasmid

    #168082

    Purpose

    Expresses Cyterm-ymScarletI to check for monomericity.

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    cyterm-ymScarletI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge