8,058 results

1. YIplac204-TC-OM45-FKBPx4

   Plasmid

   #104472

   Purpose

   Integrating plasmid for overexpression of OM45-FKBPx4.

   Depositor

   Benjamin Glick

   Article

   Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

   Insert

   OM45p (OM45 Budding Yeast)
   

   Use

   Tags

   FKBPx4- HA

   Expression

   Yeast

   Mutation

   Promoter

   TPI1

   Available since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV104

   Plasmid

   #63194

   Purpose

   pRS404 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pREP42-MCS+

   Plasmid

   #52691

   Purpose

   Same as pREP42, with better MCS

   Depositor

   Michael Nick Boddy

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Pnmt41

   Available since

   April 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRPL28(K55M) SpHIS5 (pNTI666)

   Plasmid

   #115436

   Purpose

   Expresses K55M RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K55M; MfeI site in intron

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTR1-GPD-TRPC

   Plasmid

   #190103

   Purpose

   Expression vector for fungal (Aspergillus flavus) genes based on pyrithiamine resistance (ptrA) selection

   Depositor

   Perng-Kuang Chang

   Article

   Chang et al Appl Environ Microbiol. 2012 Nov;78(21):

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. YIp ADE2-URA3

   Plasmid

   #122907

   Purpose

   ADE2-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. GAL FLAG25QDProCFPp303

   Plasmid

   #15584

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 25Q Delta Pro (HTT Human)
   

   Use

   Tags

   CFP and FLAG

   Expression

   Yeast

   Mutation

   Proline-rich region removed

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

8. YIplac204-T|C-iGFP-PH(OSH1)

   Plasmid

   #105280

   Purpose

   Overexpresses iGFP-PH(Osh1) under the TPI1 promoter. Integrating at TRP1.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   PH domain of Osh1 (SWH1 Budding Yeast)
   

   Use

   Tags

   iGFP

   Expression

   Yeast

   Mutation

   Promoter

   TPI1

   Available since

   Jan. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. RS314-SN

   Plasmid

   #17539

   Depositor

   Bert Vogelstein

   Article

   Thiagalingam et al Proc Natl Acad Sci U S A. 1995 Jun 20. 9

   Insert

   Gal1-p53 (TP53 Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 28, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pRR2

    Plasmid

    #166097

    Purpose

    Gal inducible expression of dCas9-FokI

    Depositor

    Rebecca Burgess

    Insert

    dCas9-FokI
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9-D10A, H840A

    Promoter

    Gal-inducible

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pROS16

    Plasmid

    #107930

    Purpose

    HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-ytdTomato-CaURA3

    Plasmid

    #118461

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection. *An updated version of this plasmid is available: #168054 pFA6a-link-ytdTomato-URA3.

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ytdTomato
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG306-GPD-VPH2 chr I

    Plasmid

    #41897

    Purpose

    Yeast expression of VPH2.

    Depositor

    Dan Gottschling

    Article

    Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

    Insert

    VPH2 (VPH2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Feb. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMEL13

    Plasmid

    #107919

    Purpose

    kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS416-Gal4-dCas9-VP64

    Plasmid

    #71128

    Purpose

    This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.

    Depositor

    Ronald Davis

    Article

    Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.

    Insert

    Gal4-dCas9-VP64
    

    Use

    Tags

    NLS n-terminal, N terminal Gal4 Activator domain,…

    Expression

    Yeast

    Mutation

    Promoter

    Tef1

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAD

    Plasmid

    #64747

    Purpose

    Yeast expression plasmid: ACC1 (ser659ala, ser1157ala)

    Depositor

    Jens Nielsen

    Article

    Shi et al MBio. 2014 May 6;5(3):e01130-14. doi: 10

    Insert

    ACC1 (ser659ala, ser1157ala)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJKW1148

    Plasmid

    #119029

    Purpose

    Yeast expression plasmid for PmCYP719A26

    Depositor

    Jing-Ke Weng

    Article

    Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

    Insert

    PmCYP719A26
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST-N2H-F2-empty vector

    Plasmid

    #125552

    Purpose

    N2H assay vector with nanoluc fragment2 (empty control, no Gateway cloning site, expressing nanoluc fragment2)

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment2 alone without any attached prot…

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. KM-CashGem-Xyl2-URA3-LINEAR

    Plasmid

    #174838

    Purpose

    pRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianus

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TEF1p

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. p404CYC1

    Plasmid

    #15971

    Depositor

    Fred Cross, Nicolas Buchler

    Insert

    CYC1 promoter + polylinker + CYC1 terminator (CYC1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    CYC1 promoter is between SacI and XbaI restrictio…

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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