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Plasmid#79016PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selectionDepositorTypeEmpty backboneUseTagsdouble V5 epitope tagExpressionYeastMutationPromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only
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pNB761
Plasmid#60156PurposeFirefly luciferase (Promega) driven by ADE17 promoter.DepositorInsertFLuc
UseTagsExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterADE17prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pCfB2991
Plasmid#63643PurposepCfB2991 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoternoneAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pAG416GPD-Cerulean-YPT1
Plasmid#18848DepositorInsertYPT1 (YPT1 Budding Yeast)
UseTagsCeruleanExpressionYeastMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pXP312
Plasmid#26834DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 WT
Plasmid#87599PurposeEncodes Prp5 WT for expression in yeastDepositorInsertPRP5 (PRP5 )
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNB789
Plasmid#60166PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.DepositorInsertFLuc-yEVenus-PEST
UseTagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterRNR1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-hSyntenin2b
Plasmid#89422PurposeTwo-hybrid systemDepositorInsertSDCBP2 (SDCBP2 Human)
UseTagsGAL4 DBDExpressionYeastMutation* see belowPromoterADH1Available sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pLUK
Plasmid#64184Purposeharbours marker genes LEU2, URA3 and LYS2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pED45: pRS306 pRPS2-Ste7DS-NLS-mCherry
Plasmid#68274PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherryDepositorInsertSte7DS NLS mCherry
UseTagsmCherryExpressionYeastMutationPromoterpRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
YIp211-P30-His-pol30(K127R)-3'
Plasmid#99547PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; URA3 markerDepositorInsertPOL30 (POL30 Budding Yeast)
UseTags6xHisExpressionYeastMutationK127RPromoterPOL30Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNXRVat-HACre
Plasmid#78399Purposeconstitutive Cre expression vectorDepositorInserts3xHA-nls-Cre
TKanAX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNXRVbt-HACre
Plasmid#78402Purposeconstitutive Cre expression vectorDepositorInserts3xHA-nls-Cre
HyTkAX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTL-ir
Plasmid#81102PurposeGene Tagging vector LEU/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)DepositorInserttdiRFP
UseCre/LoxTagstdiRFPExpressionYeastMutationcodon optimized second iRFP to avoid internal rec…PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTT-y
Plasmid#81110PurposeGene Tagging vector TRP/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)DepositorInsertmCitrine
UseCre/LoxTagsmCitrineExpressionYeastMutationMutated mCitrine for monomeric isoform (A206K L22…PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTT-ir
Plasmid#81112PurposeGene Tagging vector TRP/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)DepositorInserttdiRFP
UseCre/LoxTagstdiRFPExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTL-r
Plasmid#81195PurposeGene Tagging vector LEU/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)DepositorInsertmCherry
UseCre/LoxTagsmCherryExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG423GAL-ccdB-TAP
Plasmid#14269DepositorTypeEmpty backboneUseGateway destinationTagsTAPExpressionYeastMutationPromoterAvailable sinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
GAL-CDC12(E368Q)
Plasmid#1164DepositorInsertCDC12 (CDC12 Budding Yeast)
UseTagsExpressionYeastMutationE368QPromoterGALAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDS297
Plasmid#34986DepositorInsertcpPDZ
UseTagsExpressionYeastMutationPromoterGAL1Available sinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pJRL2 PHO5(TATA-A1T6)pr ECFP (EB#1560)
Plasmid#14846DepositorInsertPHO5 promoter TATA-A1T6 (PHO5 Budding Yeast)
UseTagsECFPExpressionYeastMutationTATA-A1T6. TATA box mutated from TATATAAG to AATA…PromoterAvailable sinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
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RMC
Plasmid#1173DepositorUseTagsExpressionYeastMutation*(see below). Contains Rnq aa's 153-393 and…PromoterAvailable sinceJan. 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
pHU
Plasmid#64172Purposeharbours marker genes HIS3 and URA3DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG424GPD-ECFP-ccdB
Plasmid#14296DepositorTypeEmpty backboneUseGateway destinationTagsECFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
416Gal-FUS-R514S-YFP
Plasmid#29617DepositorInsertFUS (FUS Human)
UseTagsYFPExpressionYeastMutationR514SPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAG423GPD-Cerulean-ccdB
Plasmid#14414DepositorTypeEmpty backboneUseGateway destinationTagsCeruleanExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinADE E171Q GFP
Plasmid#32128DepositorInsertTorsinA (TOR1A Human)
UseTagsGFP and Kar2ExpressionYeastMutationdeletion of E at 302 or 303, E171QPromoterAvailable sinceOct. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-ScDcr1deltaN
Plasmid#32038DepositorInsertDrc1(111-610)
UseTagsExpressionYeastMutationPromoterGAL1Available sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSB819-PKR-hum-FFA
Plasmid#20048DepositorInsertProtein kinase R (EIF2AK2 Human)
UseTagsExpressionYeastMutationL394F, S492APromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTH340-SUP45(1-242)-GalBD
Plasmid#29740DepositorInsertSUP45 (SUP45 Budding Yeast)
UseTagsGal Activation domain and MycExpressionYeastMutationCodons 1-242 of the yeast SUP45 gene onlyPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLM
Plasmid#64175Purposeharbours marker genes LEU2 and MET17DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG304GPD-EYFP-ccdB
Plasmid#14328DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG305GPD-ccdB-ECFP
Plasmid#14162DepositorTypeEmpty backboneUseGateway destinationTagsECFPExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only