8,043 results

1. pDS1U-PKS12

   Plasmid

   #70150

   Purpose

   Fusarium graminearum PKS12 encoded on CEN/ARS yeast shuttle vector with DS1U selection marker

   Depositor

   Morten Sommer

   Article

   Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

   Insert

   PKS12 (PKS12 Fusarium graminearum)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CUP1

   Available since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAV115.loxP

   Plasmid

   #63187

   Purpose

   pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAV116.loxP

   Plasmid

   #63188

   Purpose

   pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. M34 Xdsh PDZ bait-pGBT9

   Plasmid

   #17190

   Depositor

   Randall Moon

   Article

   Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

   Insert

   Xdsh (dvl2.L Frog)
   

   Use

   Tags

   Gal4-DBD

   Expression

   Yeast

   Mutation

   Dsh bp 343-1191

   Promoter

   Available since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

5. YIplac211-RPL13A-FKBPx4

   Plasmid

   #104474

   Purpose

   Plasmid for pop-in/pop-out replacement of RPL13A with an FKBPx4-tagged version.

   Depositor

   Benjamin Glick

   Article

   Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

   Insert

   RPL13Ap (RPL13A Budding Yeast)
   

   Use

   Tags

   FKBPx4-HA

   Expression

   Yeast

   Mutation

   includes the last 354 bp of RPL13A only

   Promoter

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. KAP121-FLU (115)

   Plasmid

   #24047

   Depositor

   Michael Rout

   Insert

   KAP121 (PSE1 Budding Yeast)
   

   Use

   Tags

   FLU

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 29, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pGBT9-hSyntenin2a

   Plasmid

   #89421

   Purpose

   Two-hybrid system

   Depositor

   Fritz Rathjen

   Article

   Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

   Insert

   SDCBP2 (SDCBP2 Human)
   

   Use

   Tags

   GAL4 DBD

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDR-AmTrac1;2

   Plasmid

   #47765

   Purpose

   expresses AmTrac1;2 in yeast

   Depositor

   Wolf Frommer

   Article

   De Michele et al eLife. 2013 Jul 2;2:e00800.

   Insert

   AmTrac1;2
   

   Use

   Tags

   mcpGFP

   Expression

   Yeast

   Mutation

   Promoter

   PMA

   Available since

   Sept. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pRS314-PRP16

   Plasmid

   #89971

   Purpose

   Prp16 in a trp backbone complements prp16 genomic deletion

   Depositor

   Christine Guthrie

   Article

   Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

   Insert

   PRP16 (PRP16 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   none

   Promoter

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDR-AmTrac-LS

    Plasmid

    #47767

    Purpose

    expresses AmTrac-LS in yeast

    Depositor

    Wolf Frommer

    Article

    De Michele et al eLife. 2013 Jul 2;2:e00800.

    Insert

    AmTrac-LS
    

    Use

    Tags

    mcpGFP

    Expression

    Yeast

    Mutation

    Promoter

    PMA

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

11. Osa-pCAMBIA-1301-STTM390

    Plasmid

    #84202

    Purpose

    Disturb the expression of miR390 in rice

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR390
    

    Use

    Tags

    Expression

    Bacterial, Plant, and Yeast…

    Mutation

    Promoter

    2x35S

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

12. Osa-pCAMBIA-1301-STTM393

    Plasmid

    #84203

    Purpose

    Disturb the expression of miR393 in rice

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR393
    

    Use

    Tags

    Expression

    Bacterial, Plant, and Yeast…

    Mutation

    Promoter

    2x35S

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

13. Osa-pCAMBIA-1301-STTM156

    Plasmid

    #84194

    Purpose

    Disturb the expression of miR156 in rice

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR156
    

    Use

    Tags

    Expression

    Bacterial, Plant, and Yeast…

    Mutation

    Promoter

    2x35S

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pPS1307

    Plasmid

    #8912

    Depositor

    Pamela Silver

    Article

    Henry et al Mol Cell Biol. 1996 Jul . 16(7):3668-78.

    Insert

    HMT1 (HMT1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG424GPD-ccdB-DsRed

    Plasmid

    #14368

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    DsRed

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAG424GAL-ccdB-ECFP

    Plasmid

    #14175

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNC861

    Plasmid

    #20391

    Depositor

    Beverly Errede

    Article

    Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

    Insert

    CFP-reporter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    X=UbiY (see schematic) linker: ek

    Promoter

    Available since

    March 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS303-pGAL-HA-TMSec66-SrtA (pKS105)

    Plasmid

    #50033

    Purpose

    Galactose-inducible expression of ER-membrane S. pyogenes SrtA in yeast

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

    Insert

    SrtA(strep)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    pGAL

    Available since

    Jan. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS316-Cwc25

    Plasmid

    #111343

    Purpose

    Cwc25 in pRS316(Ura) vector

    Depositor

    Christine Guthrie

    Insert

    CWC25
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTH751-CEN-RLuc/min53maxCFLuc

    Plasmid

    #38217

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The first 53 codons of the original FLuc gene wer…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAG415-ADH1p-HA-rap1-K9R

    Plasmid

    #84531

    Purpose

    This plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeast

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    K(43,61,69,117,240,246,527,582,651)R

    Promoter

    ADH1

    Available since

    Jan. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

22. pAG415-GPDp-EGFP-rap1-K3NR

    Plasmid

    #84533

    Purpose

    This plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeast

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    K(43,117,118)R

    Promoter

    GPD

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pAG415-GPDp-EGFP-rap1-K3CR

    Plasmid

    #84534

    Purpose

    This plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeast

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    K(462,463,651)R

    Promoter

    GPD

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

24. pAG415-GPDp-EGFP-rap1-K9R

    Plasmid

    #84535

    Purpose

    This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeast

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    K(43,61,69,117,240,246,527,582,651)R

    Promoter

    GPD

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pAG415-GPDp-EGFP-rap1-K651R

    Plasmid

    #84536

    Purpose

    This plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeast

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    K651R

    Promoter

    GPD

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pRY1004

    Plasmid

    #21788

    Depositor

    Richard Yu

    Article

    Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

    Insert

    Dig1-YFP (DIG1 A. victoria, Budding Yeast)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    contains 5' 1000bp UTR (Dig1 promoter) Di…

    Promoter

    Available since

    Sept. 18, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. YEplac181-DST1

    Plasmid

    #8624

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics 2004 Mar;166(3):1215-27.

    Insert

    DST1 (DST1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Wild type

    Promoter

    Available since

    Nov. 15, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSL10 - Brr2 del422 WT

    Plasmid

    #90260

    Purpose

    protein expression

    Depositor

    Christine Guthrie

    Article

    Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

    Insert

    BRR2
    

    Use

    Tags

    TAP

    Expression

    Yeast

    Mutation

    deleted first 422 amino acids

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAG306GAL-ccdB-DsRed

    Plasmid

    #14355

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    DsRed

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPS1889

    Plasmid

    #1361

    Depositor

    Pamela Silver

    Article

    Damelin et al Mol Cell 2000 Jan;5(1):133-40.

    Insert

    NLS-ECFP-EYFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

31. pAG423GPD-ECFP-ccdB

    Plasmid

    #14294

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAG423GPD-ccdB-EYFP

    Plasmid

    #14222

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCALex

    Plasmid

    #17265

    Depositor

    Stuart Schreiber

    Article

    Huang et al Proc Natl Acad Sci U S A. 1997 Dec 9. 94

    Type

    Empty backbone

    Use

    Tags

    LexA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

34. pAG303GPD-EYFP-ccdB

    Plasmid

    #14326

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAG305GPD-EYFP-ccdB

    Plasmid

    #14330

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAG423GPD-ccdB-TAP

    Plasmid

    #14270

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    TAP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAG415GAL-ccdB-ECFP

    Plasmid

    #14169

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. p41Neo 1-F GW

    Plasmid

    #58545

    Purpose

    CEN vector with G418 resistance marker for single Gateway insertion

    Depositor

    Leonid Kruglyak

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - delta Y

    Plasmid

    #60393

    Purpose

    Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal tyrosine (delta Y)

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB1 (TUB1 Synthetic, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    internal 6xHis (see supplement figure 4)

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

40. p2HGAL/CGR/CYC

    Plasmid

    #1218

    Depositor

    Susan Lindquist

    Article

    Nathan et al Mol Cell Biol 1995 Jul;15(7):3917-25.

    Insert

    CGR (Nr3c1 Rat)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    pRS423 with GAL promoter and CYC terminator gene…

    Promoter

    Available since

    July 12, 2006

    Availability

    Academic Institutions and Nonprofits only