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pSWAP_Entry_T2

Plasmid

#184864

Purpose

pSwap plasmid part containing the T2 sgRNA module for the Swap and Drop recombination system.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSWAP_Entry_T1

Plasmid

#184863

Purpose

pSwap plasmid part containing the T1 sgRNA module for the Swap and Drop recombination system.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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EFSp-GFP-YAP (S94A)

Plasmid

#174169

Purpose

Lentiviral vector to constitutively express TEAD-binding mutant YAP under control of the EFS promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

3x FLAG tag

Expression

Mammalian

Mutation

Serine 94 mutated to Alanine

Promoter

EFS

Available Since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV2 Nucleocapsid

Plasmid

#153201

Purpose

Expresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian cells

Depositor

Peter Klein

Article

Liu et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

Insert

Nucleocapsid from SARS-CoV-2 (N severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))

Tags

Myc tag (5X)

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro

Plasmid

#141385

Purpose

Lentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-E (E SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC18A2_STOP

Plasmid

#161163

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC18A2 (SLC18A2 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pSWAP_Entry_INS

Plasmid

#184867

Purpose

pSwap plasmid part with insert fragment module

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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dClover2-C1

Plasmid

#54577

Purpose

Localization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.

Depositor

Michael Davidson

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Type

Empty backbone

Tags

dClover2

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC15A1

Plasmid

#131874

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC15A1 (SLC15A1 Human)

Expression

Mammalian

Available Since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCLIPf-NK1R Control Plasmid

Plasmid

#101125

Purpose

Expression of CLIP-tagged Neurokinin-1 Receptor in Mammalian cells

Depositor

New England Biolabs, Ana Egana

Insert

Neurokinin-1 Receptor, Truncated (TACR1 Human)

Tags

10xHis, CLIP-tag (CLIPf), and FLAG Epitope

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pSWAP_Entry_HA

Plasmid

#184866

Purpose

pSwap plasmid part with HA homology arms

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pEMG-AIS

Plasmid

#225133

Purpose

For genome editing in P. putida KT2440, arabinose is used for the induction of I-sceI gene. Km.

Depositor

Lars Blank

Article

Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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CA2-NanoLuc Fusion Vector

Plasmid

#237164

Purpose

Express CA2-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

CA2 (CA2 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-HIS6-MBP-TEV-GG

Plasmid

#215800

Purpose

Cloning of target gene with N terminal His6 MBP tag and TEV Cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHIV-NAT-FLAG-CIP2A FL (sg2R)

Plasmid

#222624

Purpose

Lentiviral vector that expresses Flag-tagged sgRNA resistant CIP2A in mammalian cells

Depositor

Daniel Durocher

Article

Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

Insert

CIP2A (CIP2A Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF-1-alpha promoter

Available Since

July 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSNAPf-Cox8A Control Plasmid

Plasmid

#101129

Purpose

Expression of SNAP-Tagged Cox8A in Mammalian cells

Depositor

New England Biolabs, Ana Egana

Insert

Cox8A (COX8A Human)

Tags

SNAP-tag (SNAPf)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS SARS-CoV-2 BA.2 Spike

Plasmid

#185604

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.2 Spike

Depositor

Marceline Côté

Article

Tauzin et al Cell Rep. 2022 Oct 25;41(4):111554. doi:

Insert

SARS-CoV-2 BA.2 Spike (S )

Expression

Mammalian

Mutation

T19I, L24S, Δ25-27, G142D, V213G, G339D, S371F, S…

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

July 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc

Plasmid

#223559

Purpose

Alias: Gαi bONE-GO. Lentiviral vector expressing GINIP-Nluc after a low efficiency IRES downstream of YFP-CAAX placed after the promotor

Depositor

Mikel Garcia-Marcos

Article

Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457

Insert

YFP(Venus) - KRas4b - IRES* - human GINIP - linker(GGGS) - Nluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDropVn

Plasmid

#184873

Purpose

Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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mPA-GFP-TOMM20-N-10

Plasmid

#57158

Purpose

Localization: Outer Mitochondrial Membrane, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

TOMM20 (TOMM20 Human)

Tags

mPA-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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