1. hCas9

   Plasmid

   #41815

   Purpose

   Expresses human codon optimized Cas9 nuclease for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. hCas9-VPR

   Plasmid

   #68497

   Purpose

   nuclease competent SP-Cas9 fused to VPR

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   SP-Cas9-VPR
   

   Use

   Tags

   VPR

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCas9-HE-Geminin

   Plasmid

   #109402

   Purpose

   Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein and Geminin degron

   Depositor

   Jean-Paul Concordet

   Article

   Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

   Insert

   HE-Geminin
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCas9-CtIP

   Plasmid

   #109403

   Purpose

   Expresses Cas9 fusion to human CtIP protein

   Depositor

   Jean-Paul Concordet

   Article

   Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

   Insert

   CtIP (RBBP8 Human)
   

   Use

   Tags

   Expression

   Mutation

   full length CtIP

   Promoter

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCas9-Geminin

   Plasmid

   #109401

   Purpose

   Expresses Cas9 fusion to Geminin degron domain

   Depositor

   Jean-Paul Concordet

   Article

   Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

   Insert

   Geminin (GMNN Human)
   

   Use

   Tags

   Expression

   Mutation

   Geminin degron fragment

   Promoter

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCas9-HE

   Plasmid

   #109400

   Purpose

   Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein

   Depositor

   Jean-Paul Concordet

   Article

   Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

   Insert

   HDR-Enhancer domain of human CtIP protein (RBBP8 Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TFORF1349

   Plasmid

   #141815

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   POGZ (POGZ Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RCAS(A) cWnt5b (CT#851)

   Plasmid

   #13975

   Depositor

   Cliff Tabin

   Article

   Theodosiou et al Dev Biol. 2003 Jul 15. 259(2):258-71.

   Insert

   Wnt5b (WNT5B Chicken)
   

   Use

   Retroviral; Avian expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pTKhc-yibDp-GFP-cp6

   Plasmid

   #134938

   Purpose

   Low phosphate inducible circular permutation of GFP

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   GFPcp6
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHCKan-yibDp-GFPuv

   Plasmid

   #127078

   Purpose

   Low phosphate inducible yibD gene promoter expressing GFPuv reporter

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHCKan-INSyibp-Nef

    Plasmid

    #134593

    Purpose

    Low Phosphate inducible Nef

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    HIV Nef protein
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    yibD

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHCKan-yibDp-GST

    Plasmid

    #134592

    Purpose

    Low Phosphate inducible GST

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    Glutathione S-transferase
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    yibD

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHCKan-yibDp-mCherry

    Plasmid

    #127058

    Purpose

    Low phosphate inducible mCherry with N-terminal linker and His tag

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDF-yibDp-matB

    Plasmid

    #134597

    Purpose

    Low Phosphate inducible matB (malonyl-CoA synthetase)

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    Malonyl CoA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibD

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHCKan-yibDp-ald*-alaE

    Plasmid

    #134939

    Purpose

    Low phosphate inducible alanine dehydrogenase and alanine exporter

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    alanine dehydrogenase and L-alanine exporter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibD

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSMART-GFPuv

    Plasmid

    #65822

    Purpose

    GFPuv Expression, Low Phosphate Dependent E. coli Expression

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    gfpuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli insulated waaH gene promoter, low phosp…

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHCKan-yibDp-mdlC

    Plasmid

    #134590

    Purpose

    Low Phosphate inducible mdlC benzylformate decarboxylase

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    mdlC (benzylformate decarboxylase) C -terminal his tag
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHCKan-T7-CBD-hGLY

    Plasmid

    #134940

    Purpose

    T7 based expression of human glycine acyltransferase with an N-terminal chitin binding domain

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    human glycine acyltransferase 2 (GLYATL2 )
    

    Use

    Tags

    chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    t7

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHCKan-yibDp-cimA3.7

    Plasmid

    #134595

    Purpose

    Low Phosphate inducible mutant citramalate synthase

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    cimA3.7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I47V, E114V, H126Q, T204A, L238S

    Promoter

    yibDp

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHCKan-yibDp-SBS-mCherry

    Plasmid

    #134598

    Purpose

    Low Phosphate inducible SBS (bonryl-diphosphate) synthase with C-terminal mCherry)

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    SBS-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTKhc-yibDp-GFP-β20cp6

    Plasmid

    #127060

    Purpose

    Low phosphate inducible circular permutation of GFP, which is a substrate of the lon protease

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    GFPcp6-beta20
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    yibD

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHCKan-yibDp-CBD-hGLY

    Plasmid

    #134596

    Purpose

    Low Phosphate inducible hGLYAT2 with N-terminal chitin binding domain tag

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    human glycine acyltransferase 2 (GLYATL2 )
    

    Use

    Tags

    chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    yibD

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSMART-Ala1

    Plasmid

    #65814

    Purpose

    NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    NADPH Alanine Dehydrogenase (ald Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D196A, L197R

    Promoter

    Insulated waaHp from E. coli

    Available since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV4Neo-murine IL-17RA-HA

    Plasmid

    #46859

    Purpose

    expresses mouse IL-17RA

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    IL-17RA (Il17ra Mouse)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

24. pSMART-ala10

    Plasmid

    #87135

    Purpose

    NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Inserts

    NADPH Alanine Dehydrogenase (ald Synthetic)
    alanine exporter (alaE Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D196A, L197R

    Promoter

    waaHp from E. coli as part of operon with ald* an…

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. gh33

    Plasmid

    #106710

    Purpose

    expression of gRNA targeting B4GALNT3

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    B4GALNT3 (B4GALNT3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV4Neo-murine IL-17RASEFIR-HA

    Plasmid

    #46863

    Purpose

    expresses mouse IL-17RA with SEFIR deletion

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    IL-17RA-delta-SEFIR (Il17ra Mouse)
    

    Use

    Tags

    3x HA

    Expression

    Mammalian

    Mutation

    Deletion of SEFIR domain (AA 394-485)

    Promoter

    CMV

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA3.1-HA-RAD52-WT

    Plasmid

    #235676

    Purpose

    Expresses HA-tagged wild type human RAD52 in mammalian cells.

    Depositor

    Simon Powell

    Article

    Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

    Insert

    RAD52 (RAD52 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-HA-RAD52-∆C

    Plasmid

    #235677

    Purpose

    Expresses HA-tagged mutant human RAD52 in mammalian cells with a C-terminal deletion (Δ302-410 amino acids).

    Depositor

    Simon Powell

    Article

    Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

    Insert

    RAD52 (RAD52 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 302-410

    Promoter

    Available since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CL20_mEGFP_ADIPOR2_FOXJ2

    Plasmid

    #205768

    Purpose

    Express mEGFP-tagged fusion protein, ADIPOR2_FOXJ2 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    ADIPOR2_FOXJ2 (ADIPOR2, FOXJ2 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge