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Showing: 1 - 30 of 198 results

  2. pMVS142_pACT1_mCherry_Zif268_EBD_MCS_KAN

    Plasmid
    #99049
    Purpose
    Transcription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domain
    Depositor
    Barak Cohen
    Article
    Staller et al Cell Syst. 2018 Mar 1. pii: S2405-4712(1
    Inserts
    ACT1 promoter (ACT1 Budding Yeast)
    mCherry
    Zif268 DNA binding Domain
    Estrogen response domain (ESR1 Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    99049-plasmid-map-sequence-id-350965

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  6. pTACT13

    Plasmid
    #24784
    Depositor
    Tanya Parish
    Insert
    PAg85a-TetR (tetR Bacterial)
    Use
    Mycobacterial shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2010
    Availability
    Academic Institutions and Nonprofits only
    24784-plasmid-map-sequence-id-11503

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  8. pCAP03-act1p

    Plasmid
    #120233
    Purpose
    Carries promoter cassette containing act1p promoter and apramycin resistance gene that can be amplified with homology sequence for refactoring.
    Depositor
    Bradley Moore
    Article
    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-
    Insert
    promoter cassette containing act1p promoter and apramycin resistance gene
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    aac(3)IV (apramycin resistance gene)
    Promoter
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    120233-plasmid-map-sequence-id-233753

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  11. pL0_EF-act1_tr

    Plasmid
    #202171
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    act1 truncated terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202171-plasmid-map-sequence-id-405685

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  17. pPICZc-ScAct1*-thymosinB-8His

    Plasmid
    #111148
    Purpose
    Expresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37
    Depositor
    Mohan Balasubramanian
    Article
    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827
    Insert
    Act1 (ACT1 Budding Yeast)
    Use
    Pichia pastoris integration plasmid
    Tags
    Thymosin beta and a His-tag
    Expression
    Yeast
    Mutation
    Promoter
    AOX1
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111148-plasmid-map-sequence-id-214055

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  18. pACT1:Cas9-GFP, U6:sgUPRT

    Plasmid
    #122853
    Purpose
    Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene
    Depositor
    David Sibley
    Article
    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19
    Inserts
    Cas9-GFP
    U6-sgUPRT
    Use
    CRISPR; Cas9-gfp plasmid for genome editing of cr…
    Tags
    eGFP
    Expression
    Mutation
    Promoter
    Cryptosporidium parvum U6 and Cryptosporidium par…
    Available since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    122853-plasmid-map-sequence-id-238877

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  20. pACT1:Cas9-GFP, U6:sgTK

    Plasmid
    #122852
    Purpose
    Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK gene
    Depositor
    David Sibley
    Article
    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19
    Inserts
    Cas9-GFP
    U6-sgTK
    Use
    CRISPR; Cas9-gfp plasmid for genome editing of cr…
    Tags
    eGFP
    Expression
    Mutation
    Promoter
    Cryptosporidium parvum U6 and Cryptosporidium par…
    Available since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    122852-plasmid-map-sequence-id-238880

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  22. pCR4-Dact1(1250-1601)

    Plasmid
    #52046
    Purpose
    To generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.
    Depositor
    Ben Cheyette
    Article
    Suriben et al Dev Dyn. 2006 Nov;235(11):3177-83.
    Insert
    Dact1(1250-1601) (Dact1 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  25. ACT1-CUP1 9bp dist

    Plasmid
    #234131
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234131-plasmid-map-sequence-id-466522

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  26. ACT1-CUP1 12bp dist

    Plasmid
    #234130
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234130-plasmid-map-sequence-id-466519

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  27. ACT1-CUP1 27bp dist

    Plasmid
    #234129
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234129-plasmid-map-sequence-id-466539

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  28. ACT1-CUP1 15bp dist

    Plasmid
    #234128
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234128-plasmid-map-sequence-id-466529

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  29. ACT1-CUP1 42bp dist

    Plasmid
    #234127
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234127-plasmid-map-sequence-id-466531

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  30. ACT1-CUP1 50bp dist

    Plasmid
    #234112
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234112-plasmid-map-sequence-id-466054

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Showing: 1 - 30 of 198 results