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GAK-pmCherryN1
Plasmid#27695DepositorInsertGAK (Gak Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
AgAK
Plasmid#71290PurposeExpression of A. gambiae adenosine kinaseDepositorInsertAdenosine Kinase
UseTags6X HistidineExpressionBacterialMutationPromoterT7Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pc-GAKIN-Ha
Plasmid#188246PurposeExpress GAKIN in mammalian cells with Ha tagDepositorInsertGAKIN (KIF13B Human)
UseTagsHaExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
GAK_HUMAN_D0
Plasmid#79682PurposeThis plasmid encodes the kinase domain of GAK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertGAK (GAK Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
negAKARet-cyto
Plasmid#114733PurposeNegative control of AKARet-cytoDepositorInsertNegative Control of AKARet-cyto
UseTagsEGFP and sREAChetExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
GAKA-c028
Plasmid#53629Purposebacterial expression plasmidDepositorInsertGAKA
UseTagsExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
GAK gRNA (BRDN0001146687)
Plasmid#76470Purpose3rd generation lentiviral gRNA plasmid targeting human GAKDepositorInsertGAK (GAK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
GAK gRNA (BRDN0001146263)
Plasmid#76471Purpose3rd generation lentiviral gRNA plasmid targeting human GAKDepositorInsertGAK (GAK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
GAK gRNA (BRDN0001148048)
Plasmid#76472Purpose3rd generation lentiviral gRNA plasmid targeting human GAKDepositorInsertGAK (GAK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-tdTomato-FlagAkt1
Plasmid#64831PurposeLentiviral expression of murine Flag-Akt1DepositorInsertAkt1 (Akt1 Mouse)
UseLentiviralTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-tdTomato-FlagAkt1W80A
Plasmid#64833PurposecDNA of Akt1 with point mutation from Trp to Ala at position 80. This mutation confers resistance to the MK-2206 drugDepositorInsertAkt1 (Akt1 Mouse)
UseLentiviralTags3xFlagExpressionMammalianMutationPoint mutation from Trp to Ala at position 80PromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-tdTomato-FlagAkt2
Plasmid#64832PurposeLentiviral expression of murine Flag-Akt2DepositorInsertAkt2 (Akt2 Mouse)
UseLentiviralTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-tdTomato-FlagAkt2W80A
Plasmid#64834PurposecDNA of Akt2 with point mutation from Trp to Ala at position 80. This mutation confers resistance to MK-2206 drug.DepositorInsertAkt2 (Akt2 Mouse)
UseLentiviralTags3xFlagExpressionMammalianMutationPoint mutation Trp 80 to AlaPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tet-inducible mCherry reporter
Plasmid#64128PurposePhotoactivatable transcription system. mCherry reporter under the control of a tet-inducible promoterDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterTetOx13-CMVAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RTP1S
Plasmid#149364PurposeExpresses RTP1S in mammalian cells.DepositorInsertRTP1S (Rtp1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME18S-F-R-Olfr160
Plasmid#149366PurposeExpresses Olfr160 in mammalian cells.DepositorInsertOlfr160 (Olfr160 Mouse)
UseTagsFLAG and rhodopsinExpressionMammalianMutationPromoterSR alphaAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBHPS-SortA
Plasmid#21930DepositorInsertsortase
UseTagsGB1, HRV3C cleagage site, and HisExpressionBacterialMutationdeleted amino acids 1-59PromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pME18S-F-R-Olfr644
Plasmid#149365PurposeExpresses Olfr644 in mammalian cells.DepositorInsertOlfr644 (Olfr644 Mouse)
UseTagsFLAG and rhodopsinExpressionMammalianMutationPromoterSR alphaAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tet-inducible Luciferase reporter
Plasmid#64127PurposePhotoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.DepositorInsertluciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterTetOx13-CMVAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
GAL4UAS-Luciferase reporter
Plasmid#64125PurposePhotoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.DepositorInsertGAL4UAS
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterGAL4UASAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBMCS-SortA
Plasmid#21931DepositorInsertsortase (srtA Staphylococcus aureus (strain N315))
UseTagsGB1 and His-tagExpressionBacterialMutationdeleted amino acids 1-59PromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNeurog2-Luciferase reporter
Plasmid#64126PurposePhotoactivatable transcription system. Luciferase reporter under the control of the Neurog2 promoter.DepositorInsertNeurog2 promoter
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterNeurog2Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
NLSx3-CRY2PHR-p65
Plasmid#64124PurposePhotoactivatable transcription system. Expression of activator probe, which includes the photolyase homology region of CRY2 (CRY2PHR) and the transcriptional activator domain.DepositorInsertCRY2PHR-p65
UseTagsHis, NLSx3, and V5ExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
LOTUS-V/pcDNA3
Plasmid#87127PurposeBioluminescent and ratiometric voltage indicatorDepositorInsertLOTUS-V
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_Tet-inducible Luciferase reporter
Plasmid#64161PurposePhotoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.DepositorInsertsgRNA1 for Tet-inducible Luciferase reporter
UseCRISPRTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_pNeurog2-Luciferase reporter
Plasmid#64160PurposePhotoactivatable transcription system. Mammalian expression of sgRNA1 to target pNeurog2-Luciferase reporter.DepositorInsertsgRNA1 for pNeurog2-Luciferase reporter
UseCRISPRTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgRNA3_GAL4UAS-Luciferase reporter
Plasmid#64159PurposePhotoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA3 for GAL4UAS-Luciferase reporter
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
RCAS (A) Wnt3a (CT#169)
Plasmid#13976DepositorInsertWnt3a (Wnt3a Mouse)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_GAL4UAS-Luciferase reporter
Plasmid#64157PurposePhotoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA1 for GAL4UAS-Luciferase reporter
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only
