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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 59 results for: enterococcus

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Showing: 1 - 20 of 59 results

  4. pRecT_2

    Plasmid
    #167546
    Purpose
    Encodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.
    Depositor
    Howard Hang
    Publication
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Insert
    E. faecium Com15 recT under an IPTG inducible promoter, with lacI
    Expression
    Bacterial
    Promoter
    IPTG
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    167546-plasmid-map-sequence-id-332204

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  5. pCas9

    Plasmid
    #167547
    Purpose
    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.
    Depositor
    Howard Hang
    Publication
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Inserts
    chloramphenicol acetyl transferase
    pUC19 origin of replication
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    167547-plasmid-map-sequence-id-332733

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  6. pUC19-E6I4Q5

    Plasmid
    #103101
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    E6I4Q5(Cas9 coding gene from Enterococcus faecalis TX0012)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103101-plasmid-map-sequence-id-201810

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  10. pUCsRNAP

    Plasmid
    #182746
    Purpose
    Swapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmids
    Depositor
    James Collins
    Publication
    Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    small RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
    Use
    CRISPR
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    182746-plasmid-map-sequence-id-358079

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  13. pLT06

    Plasmid
    #255019
    Purpose
    Markerless deletion vector that enables manipulation of enterococcus to either insert DNA or delete DNA from the genome. Contains resistance gene for chloramphenicol resistance.
    Depositor
    Lynn Hancock
    Publication
    Thurlow et al J Bacteriol. 2009 Oct;191(20):6203-10. d
    Type
    Empty backbone
    Available since
    May 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    255019-plasmid-map-sequence-id-511528

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Showing: 1 - 20 of 59 results