We narrowed to 4,052 results for: plasmid lenti crispr

1. Lenti-sgRNA-Cre-GpNLuc-sgmMRE11

   Plasmid

   #208384

   Purpose

   Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine MRE11 gene.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   sgMRE11 (Mre11a Mouse)
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#3

   Plasmid

   #208389

   Purpose

   Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#3 gene.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   sgZBP1 (Zbp1 Mouse)
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Lenti-sgRNA-Cre-GpNLuc-sgmMLKL

   Plasmid

   #208391

   Purpose

   Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine MLKL gene.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   sgMLKL (Mlkl Mouse)
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd

   Plasmid

   #216170

   Purpose

   Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- FOXA.bs- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd

   Plasmid

   #216172

   Purpose

   Express the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- Control- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd

   Plasmid

   #216171

   Purpose

   Express the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- PRDM1.bs- ZEB2.locus (PRDM1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lenti-sgRNA-Cre-GpNLuc-sgmcGAS

   Plasmid

   #208385

   Purpose

   Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine cGAS gene.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   sgcGAS (Cgas Mouse)
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lenti-sgRNA-Cre-GpNLuc-sgmSTING

   Plasmid

   #208386

   Purpose

   Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine STING gene.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   sgSTING (Sting1 Mouse)
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Lenti-sgRNA-Cre-GpNLuc-sgmATM

   Plasmid

   #208392

   Purpose

   Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ATM gene.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   sgATM (Atm Mouse)
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR

    Plasmid

    #207355

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    RNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)
    

    Use

    Lentiviral

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Lenti-CMV-MLH1dn-IRES-puroR

    Plasmid

    #207354

    Purpose

    Lentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    MutL homolog 1 (MLH1 Human)
    

    Use

    Lentiviral

    Mutation

    Δ754-756 dominant negative mutant

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid

    #163643

    Purpose

    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    

    Use

    Lentiviral

    Tags

    Luc2-P2A-tdTomato-T2A

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    EFS and pU6

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOT_4 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-LTBR

    Plasmid

    #181973

    Purpose

    Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    FMC6.3-BBz CAR; LTBR (LTBR Synthetic, Human)
    

    Use

    Lentiviral

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOT_3 - lenti-EFS-FMC6.3-28z-2A-puro-2A-LTBR

    Plasmid

    #181972

    Purpose

    Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    FMC6.3-28z CAR; LTBR (LTBR Synthetic, Human)
    

    Use

    Lentiviral

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)

    Plasmid

    #207356

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    N1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)
    

    Use

    Lentiviral

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19)

    Plasmid

    #207357

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    L227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)
    

    Use

    Lentiviral

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}

    Plasmid

    #184381

    Purpose

    This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

    Depositor

    Ori Bar-Nur

    Article

    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

    Insert

    Cas9, two gRNAs targeting dystrophin
    

    Use

    Lentiviral

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR

    Plasmid

    #181975

    Purpose

    Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    FMC6.3-BBz CAR; tNGFR (NGFR Synthetic, Human)
    

    Use

    Lentiviral

    Mutation

    NGFR truncation: aa 11-276

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR

    Plasmid

    #181974

    Purpose

    Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    FMC6.3-28z CAR; tNGFR (NGFR Synthetic, Human)
    

    Use

    Lentiviral

    Mutation

    NGFR truncation: aa 11-276

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Multiplex-CRISPR-miR-200FAM-T2A-GFP

    Plasmid

    #179856

    Purpose

    to delete miR200 family genes including miR-141, miR-200c, miR-429 and miR-200b

    Depositor

    Brendan Jenkins

    Article

    Yu et al Oncogene. 2021 Oct 19. pii: 10.1038/s413

    Insert

    hsa-miR-200 family (miR-200b, miR-200a, miR-429, miR-141 and miR-200c ) (MIR141, MIR200B, MIR429, MIR200C, MIR200A Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TMPRSS2 g2

    Plasmid

    #153016

    Purpose

    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing mCherry

    Depositor

    Jason Sheltzer

    Insert

    TMPRSS2 gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Available Since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TMPRSS2 g3

    Plasmid

    #153017

    Purpose

    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing mCherry

    Depositor

    Jason Sheltzer

    Insert

    TMPRSS2 gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Available Since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TMPRSS2 g1 + Cas9

    Plasmid

    #153015

    Purpose

    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

    Depositor

    Jason Sheltzer

    Insert

    Cas9 + TMPRSS2 gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP2

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TMPRSS2 g4 + Cas9

    Plasmid

    #153018

    Purpose

    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

    Depositor

    Jason Sheltzer

    Insert

    Cas9 + TMPRSS2 gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP2

    Available Since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ACE2 g2

    Plasmid

    #153012

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

    Depositor

    Jason Sheltzer

    Insert

    ACE2 gRNA (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ACE2 g3

    Plasmid

    #153013

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

    Depositor

    Jason Sheltzer

    Insert

    ACE2 (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ACE2 g1 + Cas9

    Plasmid

    #153011

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

    Depositor

    Jason Sheltzer

    Insert

    Cas9 + ACE2 gRNA (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP2

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pXPR_016

    Plasmid

    #107143

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Hygro resistance from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    David Root

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ACE2 g4 + Cas9

    Plasmid

    #153014

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

    Depositor

    Jason Sheltzer

    Insert

    Cas9 + ACE2 gRNA (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    TagBFP2

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pXPR_118

    Plasmid

    #113667

    Purpose

    Lentiviral vector expressing dCas9

    Depositor

    John Doench

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Mutation

    catalytically inactive

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pXPR_045

    Plasmid

    #107144

    Purpose

    cloning of a sgRNA

    Depositor

    David Root

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pXPR_047

    Plasmid

    #107145

    Purpose

    measures Cas9 activity

    Depositor

    David Root

    Insert

    GFP + sgRNA for GFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRDA_887

    Plasmid

    #245329

    Purpose

    Cas12a CRISPRa cloning vector; contains NbALFA, p65

    Depositor

    David Root, John Doench

    Type

    Empty backbone

    Use

    Lentiviral

    Mutation

    None

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLKO5d.SFFV.Cas9.P2A.BSD

    Plasmid

    #181981

    Purpose

    Lentiviral Cas9 expression

    Depositor

    Jan-Henning Klusmann

    Insert

    Cas9 (cas9 Streptococcus pyogenes (strain: NGAS638))
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKM808

    Plasmid

    #134181

    Purpose

    Lentiviral sgRNA plasmid with blasticidin resistance

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHR Ef1a-dCas9-BFP-KRAB

    Plasmid

    #217304

    Purpose

    lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter

    Depositor

    Luke Gilbert

    Insert

    dCas9-tagBFP-KRAB
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Promoter

    Ef1alpha

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. FU_tetO_dCas9-VP192-T2A-EGFP

    Plasmid

    #121173

    Purpose

    Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.

    Depositor

    Timo Otonkoski

    Insert

    dCas9-VP192-T2A-EGFP
    

    Use

    CRISPR and Lentiviral; Inducible by doxycycline

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLX_TRC311

    Plasmid

    #113668

    Purpose

    Gateway destination vector for lentiviral expression

    Depositor

    John Doench

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. CROPSeq-PP7-p65-HSF

    Plasmid

    #196719

    Purpose

    CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid

    #106477

    Purpose

    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge