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Showing: 451 - 500 of 2918 results

  8. pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA

    Plasmid
    #184636
    Purpose
    Expresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion
    Depositor
    Nicolas Tritsch
    Article
    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:
    Insert
    Gat1 (Slc6a1 Mouse)
    Use
    AAV
    Tags
    Expression
    Mutation
    Gat1 gene with Arginine 69 changed to Lysine, to …
    Promoter
    EF1a
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184636-plasmid-map-sequence-id-369063

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  12. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

    Plasmid
    #20297
    Purpose
    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    humanized ChR2 gene with histidine 134 changed to…
    Promoter
    EF1alpha
    Available since
    June 23, 2009
    Availability
    Academic Institutions and Nonprofits only
    20297-plasmid-map-sequence-id-354478

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  13. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

    Plasmid
    #20298
    Purpose
    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    humanized ChR2 gene with histidine 134 changed to…
    Promoter
    EF1alpha
    Available since
    May 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    20298-plasmid-map-sequence-id-191904

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  14. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

    Plasmid
    #66708
    Purpose
    expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed
    Depositor
    Charles Gersbach
    Insert
    S.Pyogenes VP64-dCas9-VP64
    Use
    Lentiviral and Synthetic Biology
    Tags
    VP64
    Expression
    Mutation
    D10A and H840A
    Promoter
    hUbC
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    66708-plasmid-map-sequence-id-449286

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  15. pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)

    Plasmid
    #154840
    Purpose
    Insertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.
    Depositor
    Patrick Phillips
    Article
    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.
    Insert
    homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Worm
    Mutation
    HYGR∆ encodes aa 1-226
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    154840-plasmid-map-sequence-id-307910

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  18. pOTTC1789 - pAAV (flox-stop) mGrid1 390F gRNA A EF1a eGFP-KASH

    Plasmid
    #131684
    Purpose
    An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a Cre-dependent guide RNA for mGrid1
    Depositor
    Christopher Richie
    Article
    Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10
    Inserts
    EGFP-KASH
    SpCas9 sgRNA vs mouse GRID1
    Use
    AAV
    Tags
    KASH
    Expression
    Mutation
    Promoter
    EF1a and mU6-LSL (Cre dependent)
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    131684-plasmid-map-sequence-id-437146

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  32. p240 pPr-CREM/Ins

    Plasmid
    #8405
    Purpose
    Androgen-inducible expression of Cre, with chimeric/β-globin intron within the Cre coding sequence and chicken globin insulators.
    Depositor
    Jeffrey Green
    Article
    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56
    Insert
    probasin driven CREM with insulators
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    modified Cre
    Promoter
    Probasin promoter
    Available since
    April 28, 2006
    Availability
    Academic Institutions and Nonprofits only

  33. p236 pPr-CREM

    Plasmid
    #8404
    Purpose
    Expression of Cre from tissue-specific, androgen-inducible probasin promoter, with chimeric/β-globin intron within the Cre coding sequence.
    Depositor
    Jeffrey Green
    Article
    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56
    Insert
    probasin driven CREM
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    modified Cre
    Promoter
    Probasin promoter
    Available since
    April 28, 2006
    Availability
    Academic Institutions and Nonprofits only

  37. pENN.AAV.hSyn.Cre.hGH

    Plasmid
    #105555
    Purpose
    AAV expression of Cre from hSyn promoter
    Depositor
    James M. Wilson
    Has Service
    AAV9
    Insert
    Cre
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105555-plasmid-map-sequence-id-202452

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  38. pENTR-CreERT2

    Plasmid
    #139547
    Purpose
    CreERT2 ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    CreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139547-plasmid-map-sequence-id-274672

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  45. pAAV.GFAP.Cre.WPRE.hGH

    Plasmid
    #105550
    Purpose
    AAV expression of Cre from GFAP promoter
    Depositor
    James M. Wilson
    Has Service
    AAV PHP.eB and AAV5
    Insert
    Cre
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    GFAP
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105550-plasmid-map-sequence-id-202448

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  46. pAAV.GfaABC1D.PI.Cre.SV40

    Plasmid
    #105603
    Purpose
    AAV expression of Cre from GfaABC1D promoter
    Depositor
    Baljit Khakh
    Insert
    Cre
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    GfaABC1D
    Available since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    105603-plasmid-map-sequence-id-378896

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  47. pENTR-Cre

    Plasmid
    #139545
    Purpose
    Cre ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    Cre
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139545-plasmid-map-sequence-id-274742

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  48. pENTR-CreStopDeleted

    Plasmid
    #139546
    Purpose
    Cre ORF without stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    Cre
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139546-plasmid-map-sequence-id-274745

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  49. pENN.AAV.CMVs.Pl.Cre.rBG

    Plasmid
    #105537
    Purpose
    AAV expression of Cre from CMV promoter
    Depositor
    James M. Wilson
    Has Service
    AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    Cre
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105537-plasmid-map-sequence-id-202428

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  50. pENN.AAV.hSyn.Cre.WPRE.hGH

    Plasmid
    #105553
    Purpose
    AAV expression of Cre from hSyn promoter
    Depositor
    James M. Wilson
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    Cre
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105553-plasmid-map-sequence-id-202451

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Showing: 451 - 500 of 2918 results