We narrowed to 2,329 results for: PUC19

1. pUC19-hsg(G:H)

   Plasmid

   #138259

   Purpose

   Expression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter system

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   mCherry targeting sgRNAs G and H
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-hsg(NT:NT)

   Plasmid

   #138260

   Purpose

   Expression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter system

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   Nontargeting sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. puC19 preACT1(WTdel6)

   Plasmid

   #111324

   Purpose

   for T7 transcription of wt actin pre-mRNA (del6)

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Expression

   Bacterial

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. puC19 preACT1(AcAc)

   Plasmid

   #111327

   Purpose

   T7 transcription

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Expression

   Bacterial

   Mutation

   3' splice site AcAc mutation in intron

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. puC19 preACT1(BrG)

   Plasmid

   #111326

   Purpose

   T7 transcription

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Expression

   Bacterial

   Mutation

   branch site A to G mutation in intron

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. puC19 preACT1(BrC)

   Plasmid

   #111325

   Purpose

   T7 transcription

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Expression

   Bacterial

   Mutation

   branch site A to C mutation in intron

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19 gene 60 to gap

   Plasmid

   #49158

   Purpose

   Template for generating gene 60 mRNA, sequence 5' of coding gap only

   Depositor

   Nils Walter

   Article

   Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

   Insert

   Bacteriophage T4 gene 60
   

   Mutation

   Coding gap and downstream sequence have been del…

   Promoter

   T7

   Available Since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19 - T7 pro - IRES - EGFP

   Plasmid

   #138586

   Purpose

   Express EGFP with an upstream T7 promoter in pUC19 backbone

   Depositor

   Fei Chen

   Article

   Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   EGFP
   

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

   Plasmid

   #228253

   Purpose

   pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
   

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   U6

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

    Plasmid

    #171129

    Purpose

    LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

    Depositor

    Benjamin Kleinstiver

    Article

    Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

    Insert

    U6 promoter entry plasmid for LwaCas13a crRNA cloning
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19_msfGFP-EWSR1-repair-tempate

    Plasmid

    #237682

    Purpose

    For knock-in msfGFP to EWSR1 locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    CRISPR

    Tags

    msfGFP

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19-link-mCherry-FLAG:cat

    Plasmid

    #211831

    Purpose

    Plasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.

    Depositor

    Peng Chen

    Insert

    mCherry-FLAG-cat
    

    Expression

    Bacterial

    Mutation

    NA

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19_msfGFP-SRRM2-repair-template

    Plasmid

    #238247

    Purpose

    For knock-in msfGFP to SRRM2 locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    SRRM2
    

    Use

    CRISPR

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-link-sfGFP-FLAG:Kan

    Plasmid

    #211833

    Purpose

    Plasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.

    Depositor

    Peng Chen

    Insert

    sfGFP-FLAG-Kan
    

    Expression

    Bacterial

    Mutation

    NA

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-link-mVenus-FLAG:Kan

    Plasmid

    #211830

    Purpose

    Plasmid template that encodes the fluorescent protein mVenus with a FLAG tag next to kanamycin resistance gene. This template was used to copy out mVenus-FLAG-Kan for genetic tagging.

    Depositor

    Peng Chen

    Insert

    mVenus-FLAG-Kan
    

    Expression

    Bacterial

    Mutation

    NA

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19.TRIM28.FKBP.knock-in.mRuby2

    Plasmid

    #185008

    Purpose

    For knocking in FKBP F36V P2A mRuby2

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    FKBP F36V P2A mRuby2
    

    Use

    Cloning

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-URA3-3'SEC13-GFP

    Plasmid

    #25445

    Depositor

    Benjamin Glick

    Article

    Rossanese et al J Cell Biol. 1999 Apr 5. 145(1):69-81.

    Insert

    Sec13-Green Fluorescent Protein
    

    Expression

    Yeast

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. p1977 (pUC19-PTDH3-PTEF1)

    Plasmid

    #78230

    Purpose

    Example plasmid containing a bidirectional promoter of pTDH3 and pTEF1

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    Bidirectional promoters pTDH3-pTEF1
    

    Expression

    Bacterial

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pUC19.TRIM28.FKBP.knock-in.mCerulean

    Plasmid

    #185009

    Purpose

    For knocking in FKBP F36V P2A mCerulean

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    FKBP F36V P2A mCerulean
    

    Use

    Cloning

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19-link-PAmCherry-FLAG:cat

    Plasmid

    #211832

    Purpose

    Plasmid template that encodes the fluorescent protein PAmCherry1 with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out PAmcherry1-FLAG-cat for genetic tagging.

    Depositor

    Peng Chen

    Insert

    PAmCherry-FLAG-cat
    

    Expression

    Bacterial

    Mutation

    NA

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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