We narrowed to 13,716 results for: cas9 genes

1. pCAS9id

   Plasmid

   #141251

   Purpose

   Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Article

   Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 (Cas9), pSNR52 (gRNA)

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAS9cd

   Plasmid

   #141252

   Purpose

   Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Article

   Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 (Cas9), pSNR52 (gRNA)

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUCas9GFP

   Plasmid

   #85555

   Purpose

   Expresses Cas9 with green fluorescent reporter

   Depositor

   Marco Herold

   Insert

   humanised S.pyogenes Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   hUBC

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAS9c

   Plasmid

   #141250

   Purpose

   Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Article

   Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 (Cas9), pSNR52 (gRNA)

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAS9i_TRP

   Plasmid

   #141253

   Purpose

   Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 (Cas9), pSNR52 (gRNA)

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. EC2_3_dCas9_Mxi1_sgRNA

   Plasmid

   #163708

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   Mxi1+SV40 NLS
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMYCO1_SpdCas9_pmcDA1

   Plasmid

   #204615

   Purpose

   CRISPR cytosine deaminase replicative plasmid for Mycoplasma mycoides subsp. mycoides

   Depositor

   Pascal Sirand-Pugnet

   Article

   Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9_U6_sgRNA_Mettl3C

   Plasmid

   #165420

   Purpose

   sgRNA construct for targeting Mettl3 C-terminus

   Depositor

   Neil Brockdorff

   Article

   Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

   Insert

   PspCas9 gRNA targeting Mettl3 C-terminus (Mettl3 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. EC2_10_dCas9_VPR_HC_sgRNA

   Plasmid

   #163712

   Purpose

   Yeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   VPR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. VPR_dCas9

    Plasmid

    #134601

    Purpose

    Express CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 marker

    Depositor

    Lasse Ebdrup Pedersen

    Article

    Karottki et al Biotechnol Bioeng. 2020 Feb;117(2):593-5

    Insert

    VPR-dCas9-2A-ZsGreen-DR
    

    Use

    Tags

    VPR and ZsGreen1-DR

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122089

    Purpose

    U6 driven SpCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMEG_YLCas9_empty

    Plasmid

    #117826

    Purpose

    CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica

    Depositor

    Michael Sauer

    Article

    Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

    Insert

    YlpTEF_hCas9_YlCyc1TT
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. NAX_CAGGS_CAS9_VPR_Puro

    Plasmid

    #167895

    Purpose

    PiggyBac compatible plasmid expressing Cas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. NmCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122091

    Purpose

    U6 driven NmCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    NmCas9 tracrRNA (NEWENTRY S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHdCas9

    Plasmid

    #137080

    Purpose

    Expression of Himar-dCas9 in bacteria

    Depositor

    Harris Wang

    Article

    Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

    Insert

    Himar-dCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. xCas9

    Plasmid

    #138565

    Purpose

    Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    xCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCjeCas9

    Plasmid

    #172215

    Purpose

    Expresses Campylobacter jejuni Cas9 (CjeCas9) in bacterial cells

    Depositor

    John van der Oost

    Article

    Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi

    Insert

    Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
    

    Use

    Tags

    Histag

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. NAX_CAGGS_CAS9_Puro

    Plasmid

    #167863

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pThermoCas9i_ctrl

    Plasmid

    #100982

    Purpose

    Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module

    Depositor

    John van der Oost

    Article

    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

    Inserts

    Catalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    changed aspartate 8 to alanine and histidine 582 …

    Promoter

    B. coagulans DSM 1 pta promoter and B. smithii xy…

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Nsp2Cas9_AAV

    Plasmid

    #192143

    Purpose

    Expresses Nsp2Cas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

    Insert

    Nsp2Cas9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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