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pHR- flag-Lag18-iCAM-1

Plasmid

#205207

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG18

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag18 anti-GFP nanobody

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag42-iCAM-1

Plasmid

#205205

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG42

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag42 anti-GFP nanobody

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag17-iCAM-1

Plasmid

#205204

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG17

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag17 anti-GFP nanobody

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-Dual-iCas9-hALB

Plasmid

#167502

Purpose

Expression of dual inducible Cas9 with hALB promoter

Depositor

Jake Liang

Article

Park et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

Insert

Cas9

Use

CRISPR, Lentiviral, and Luciferase

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

hALB

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SauriCas9-Puro

Plasmid

#135965

Purpose

Expresses SauriCas9 and puromycin resistance genes, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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Icam 2 SaCas9 YAP sgRNA1

Plasmid

#99734

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCF287_EFS-TiCas9-P2A-Puro

Plasmid

#211646

Purpose

EFS-TiCas9-P2A-Puro. Tamoxifen-inducible SpyCas9.

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

Tamoxifen-inducible SpyCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pColdII-SIRPT1-Loxodonta Africana

Plasmid

#75351

Purpose

Nt His-tag Loxodonta Africana 89-1368

Depositor

Kalle Gehring

Insert

Sacsin Loxodonta Africana 89-1368

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pQE80L TriCatcher-RGDSP-MMP

Plasmid

#112633

Purpose

Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcher

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

TriCatcher-RGDSP-MMP

Use

Tags

His6 and TEV cleavage site

Expression

Bacterial

Mutation

central matrix metalloproteinase-cleavable sequen…

Promoter

T5

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Brevican [N294A/10R]

Plasmid

#177528

Purpose

Mammalian Expression Plasmid of anti-Brevican (Rat). Derived from hybridoma N294A/10R.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Brevican (Rattus norvegicus) recombinant mouse monoclonal antibody (Bcan Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Versican [N351/23R]

Plasmid

#128639

Purpose

Mammalian Expression Plasmid of anti-Versican (Mouse). Derived from hybridoma N351/23.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Versican (Mouse) recombinant mouse monoclonal antibody (Vcan Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Icam 2 SaCas9 YAP sgRNA3

Plasmid

#99736

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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1203M-CMV-dDiCas7-11-bGHpolyA

Plasmid

#220957

Purpose

Deactivated DiCas7-11 effector with CMV promoter/enhancer

Depositor

Omar Akbari

Insert

DiCas7-11

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-Brevican [N294A/10R-2b]

Plasmid

#220429

Purpose

Mammalian Expression Plasmid of anti-Brevican (Rat) subclass-switched IgG2b R-mAb. Derived from hybridoma N294A/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Brevican (Rattus norvegicus) recombinant mouse monoclonal antibody. (Bcan Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-SauriCas9-KKH-BlastR

Plasmid

#220972

Purpose

Expresses human codon-optimized SauriCas9-KKH

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

SauriCas9-KKH

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Versican [N351/24R]

Plasmid

#211585

Purpose

Mammalian Expression Plasmid of anti-Versican (Rat) IgG2a R-mAb. Derived from hybridoma N351/24.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Versican (Rattus norvegicus) recombinant mouse monoclonal antibody. (Vcan Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR- flag-Lag29-iCAM-1

Plasmid

#205208

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG29

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag29 anti-GFP nanobody

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR- flag-Lag43-iCAM-1

Plasmid

#205206

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG43

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag43 anti-GFP nanobody

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen-Uricase

Plasmid

#182417

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-uricase fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-uricase

Use

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (E60amb)-His

Plasmid

#168043

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Use

Tags

6x Histag

Expression

Bacterial

Mutation

Glu 60 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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