We narrowed to 15,384 results for: pET

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pET-6xHis-mCherry(-6)

Plasmid

#205045

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

mCherry(-6)

Tags

MGHHHHHGG

Expression

Bacterial

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-CATdeltaHD

Plasmid

#174811

Purpose

Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CATdeltaHD (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Residues 678-787 replaced by eight-residue linker…

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET-Gate2 mazEF

Plasmid

#117117

Purpose

Destination vector with mazEF toxin antitoxin system

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Type

Empty backbone

Use

Backbone for golden gate assembly for gene target…

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pET_RGG-RGG-RGG

Plasmid

#124938

Purpose

Expression of RGG-RGG-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

His

Expression

Bacterial

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+18)

Plasmid

#199162

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+18)

Tags

MGHHHHHHGGA

Mutation

E16R, D29K, E42K, E44K, D86K, D112K, T115K, D127R…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET-APOBEC1-YTHmut

Plasmid

#200159

Purpose

For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification

Depositor

Kate Meyer

Article

Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

Insert

APOBEC1-YTHmut

Tags

6xHis, HA, and MBP

Expression

Bacterial

Mutation

Codon optimized for E coli expression

Promoter

LacZ

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 aa 1-552

Plasmid

#74614

Purpose

Expresses a truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-552 (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+12)

Plasmid

#199161

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+12)

Tags

MGHHHHHHGGA

Mutation

E16R, E44K, D86K, D112K, T115K, D127R, I138R, E15…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-LgBiT-SpyCatcher

Plasmid

#223634

Purpose

Protein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

His6, LgBiT

Expression

Bacterial

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-GST-4

Plasmid

#228986

Purpose

For bacterial expression of anti-GST nanobody GST-4, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-4

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaRIgG-2

Plasmid

#228996

Purpose

For bacterial expression of anti-rabbit IgG nanobody LaRIgG-2, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaRIgG-2

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaGIgG-12

Plasmid

#228999

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-12, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaGIgG-12

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-GST-3

Plasmid

#228985

Purpose

For bacterial expression of anti-GST nanobody GST-3, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-3

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-3

Plasmid

#172769

Purpose

Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-3

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pETSUMO2_Vitiosangium-bGSDM-thrombin

Plasmid

#222099

Purpose

For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.

Depositor

Philip Kranzusch

Article

Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

Insert

Vitiosangium-bGSDM-thrombin ORF

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pETSUMO2_Nostoc-bGSDM-WELQ

Plasmid

#222100

Purpose

For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.

Depositor

Philip Kranzusch

Article

Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

Insert

Nostoc-bGSDM-WELQ ORF

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET15-His-∆NGlpG

Plasmid

#155131

Purpose

Expresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b

Depositor

Sinisa Urban

Article

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Tags

His

Expression

Bacterial

Mutation

insert codes for GlpG residues 87-276

Promoter

T7

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pET-HIS-Sangamo

Plasmid

#40786

Purpose

for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI

Depositor

Gang Bao

Insert

Transcription Activator Like Effector Nuclease

Use

TALEN

Tags

N Terminal 6xHIS

Expression

Bacterial

Mutation

+63 C Terminus, Codon Optimized FokI Endonuclease

Promoter

T7

Available Since

Dec. 12, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pCyPet-Rac1(Q61L)

Plasmid

#22783

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Rac1 (Q61L) (RAC1 Human, A. victoria)

Tags

CyPet

Expression

Mammalian

Mutation

Rac1 Q61L.

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pET16b-MYPT1(1-314)

Plasmid

#224893

Purpose

Bacterial expression of MYPT1(1-314)

Depositor

Mathieu Bollen

Article

Claes et al Cell Chem Biol. 2023 Dec 21;30(12):1666-

Insert

myosin phosphatase targeting subunit 1 (PPP1R12A )

Expression

Bacterial

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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