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  9. pLJM1-Flag-Rheb-12Rs

    Plasmid
    #165023
    Purpose
    expression of Flag-Rheb-12Rs mutant protein in mammalian cells (all the lysine residues on Rheb except the K19 and K120 are mutated to arginines).
    Depositor
    Ken Inoki
    Article
    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d
    Insert
    Rheb-12Rs (RHEB Human)
    Use
    Lentiviral
    Tags
    flag
    Expression
    Mammalian
    Mutation
    All the lysine residues except K19 and K120 are c…
    Promoter
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165023-plasmid-map-sequence-id-326718

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  15. pMCh-1522

    Plasmid
    #82428
    Purpose
    Plasmid for expression of GST-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 CED 11W11A
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    amino acids 1-1158 of nvGW182 were deleted and Tr…
    Promoter
    EF1A
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82428-plasmid-map-sequence-id-156193

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  16. pMCh-1526

    Plasmid
    #82429
    Purpose
    Plasmid for expression of NHA-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 CED 11W11A
    Use
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    amino acids 1-1158 of nvGW182 were deleted and Tr…
    Promoter
    CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82429-plasmid-map-sequence-id-156191

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  18. pcdna3 T7 Jip1b(471-707)(C478S, C667S and C699T)

    Plasmid
    #52129
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.
    Insert
    Jnk interacting protien (Jip1b)(471-707)(M1+2+6) (Mapk8ip1 Mouse)
    Use
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-470 and changed C478S, C667…
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pcdna3 T7 Jip1b(471-707)(C478S, C643S and C696T)

    Plasmid
    #52130
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.
    Insert
    Jnk interacting protein Jip1b(471-707)(M1+3+6) (Mapk8ip1 Mouse)
    Use
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-470 and changed C478S, C643…
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pcdna3 T7 Jip1b(471-707)(C478S, C631S and C699T)

    Plasmid
    #52131
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.
    Insert
    Jnk interacting protein (Jip1b)(471-707)(M1+4+6) (Mapk8ip1 Mouse)
    Use
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-470 and changed C478S, C631…
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  21. pcdna3 T7 Jip1b(471-707)(C478S, C631S, C643S, C667S and C699T)

    Plasmid
    #52133
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.
    Insert
    JNK interacting protein-1b (JIP-1b)(471-707)(M1-5) (Mapk8ip1 Mouse)
    Use
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acid 1-470 and C478S, C631S, C643S,…
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  22. pcdna3 T7 Jip1b(282-707)(C605S, C631S, C643S and C696T)

    Plasmid
    #52134
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.
    Insert
    JNK interacting protein-1b (JIP-1b)(282-707)(M1-5) (Mapk8ip1 Mouse)
    Use
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acid 1-281 and C605S, C631S, C643S …
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  27. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid
    #182287
    Purpose
    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    Use
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182287-plasmid-map-sequence-id-363047

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  28. EGF-bio-His

    Plasmid
    #53340
    Purpose
    Expresses full-length Pro-epidermal growth factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    EGF (EGF Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53340-plasmid-map-sequence-id-83070

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  30. eMscL G22S

    Plasmid
    #107455
    Purpose
    eMscL G22S is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif
    Depositor
    Francesco Difato
    Article
    Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393
    Insert
    bacterial mechanosensitive ion channel of large conductance (mscL Escherichia coli)
    Use
    Adeno-associated viral
    Tags
    Kir2.1 ER export signal (FCYENEV) and tdTomato fl…
    Expression
    Mammalian
    Mutation
    Bearing a glycine to serine substitution at posit…
    Promoter
    human synapsin 1
    Available since
    April 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    107455-plasmid-map-sequence-id-207549

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Showing: 3961 - 4000 of 84526 results