We narrowed to 7,491 results for: RAP

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pDM1081

Plasmid

#108981

Purpose

Knockout cassette Hygromycin

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

Knockout cassette Hygromycin

Use

Cre/Lox; Dictyostelium discoideum knockout vector

Promoter

coaA

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDCC6

Plasmid

#59985

Purpose

Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

Depositor

Peter Duchek

Article

Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

Inserts

Cas9
U6-2>sgRNA

Use

CRISPR

Tags

3xFLAG

Promoter

U6-96Ab and hsp70Bb

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-mRFP1

Plasmid

#21315

Purpose

MoMLV-based Retroviral vector, expresses mRFP1

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

monomeric Red Fluorescence Protein 1

Use

Retroviral

Expression

Mammalian

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pHR myc-MBP-SspB-iRFP-NLS

Plasmid

#223286

Purpose

An SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system.

Depositor

Jared Toettcher

Article

Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

Insert

Myc-MBP-SspB-irFP-NLS

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pTriEx-NTOM20-mVenus-Zdk1

Plasmid

#81010

Purpose

Expression of Zdk1 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDM1047

Plasmid

#108990

Purpose

Inducible expression vector (dox-on), N-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum inducible expression vec…

Tags

GFP

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTcI42-Wasabi

Plasmid

#86118

Purpose

mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI42

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TcI42
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

A67T, K6N, S33T, Y61H, L119P, F122C

Promoter

pR/pL and pR/pL, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDNR-mCherry-T2A-3xPuroR-Frame 1

Plasmid

#206992

Purpose

Donor plasmid for Frame 1 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

Depositor

Alejandro Chavez

Article

Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDNR-mCherry-T2A-3xPuroR-Frame 2

Plasmid

#206993

Purpose

Donor plasmid for Frame 2 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

Depositor

Alejandro Chavez

Article

Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 V416I

Plasmid

#81045

Purpose

mammalian expression of LOV V416I

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV V416I

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416I

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPhlF-BCD20-sfGFP-ssrA P64U23C39T13 v1-1 (462)

Plasmid

#70741

Purpose

1 color strong reporter used in study

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

sfGFP-ssrA

Available Since

Feb. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pUV15-pHGFP

Plasmid

#70045

Purpose

encodes a pH-sensitive ratiometric GFP (pH-GFP), which allows for noninvasive measurements of intrabacterial pH in live cells

Depositor

Sabine Ehrt

Article

Vandal et al Nat Med. 2008 Aug;14(8):849-54. doi: 10.

Insert

phGFP

Use

Mycobacterium tuberculosis expression vector

Promoter

mycobacterial promoter Psmyc

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_Tbet_T2A_GFP_PGK_mCherry

Plasmid

#85431

Purpose

Lentiviral vector for Gal4 inducible Tbet T2A GFP and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human Tbet

Use

Lentiviral

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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Rogi2-CMV-mRuby-bGH

Plasmid

#179861

Purpose

Targets Rogi2 genomic region with HDR and expresses mRuby

Depositor

Sai Reddy

Article

Aznauryan et al Cell Rep Methods. 2022 Jan 14;2(1):10015

Insert

mRuby

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyTagMBP

Plasmid

#35050

Depositor

Mark Howarth

Article

Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

Insert

SpyTagMBP

Tags

His6

Expression

Bacterial

Mutation

Simple peptide tag added to the N-terminus of MBP…

Promoter

T7 promoter

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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mIL12-N1

Plasmid

#123139

Purpose

Mammalian expression of murine cytokine IL-12 under CMV promoter

Depositor

Jordan Green

Article

Tzeng et al Proc Natl Acad Sci U S A. 2020 Feb 7. pi

Insert

Murine IL12 tandem p40 p35

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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TOPO mKate2

Plasmid

#68441

Purpose

TOPO construct expressing mKate2 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mKate2

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Ii-Str_ssSBP-EGFP

Plasmid

#65277

Purpose

to synchronize transport of secretory soluble GFP from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

EGFP

Tags

IL-2 signal sequence and Streptavidin Binding Pr…

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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Flag pLJM1 RagB wt

Plasmid

#19313

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pH6HTC_CCDC124-Halo

Plasmid

#175328

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

CCDC124 (CCDC124 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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