We narrowed to 2,732 results for: SAB;

1. MADR pDonor-H3F3A-G34R-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

   Plasmid

   #131463

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A G34R, PDGFRA D842V, and TRP53

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   H3F3A-G34R-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   Trp53-V5, H3F3A-EGFP-AU1

   Expression

   Mammalian

   Mutation

   H3F3A G34R, Pdgfra D842V

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MADR pDonor TagBFP2-V5-HRAS G12VWPRE

   Plasmid

   #129420

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   TagBFP2-V5-HRAS G12V (HRAS Human, Entacmaea quadricolor (TagBFP))
   

   Use

   Mosaic analysis for dual recombinase-mediated cas…

   Tags

   TagBFP2-V5 tag

   Expression

   Mutation

   G12V

   Promoter

   none (3x polyA to mitigate episomal expression)

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE

   Plasmid

   #131730

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strain

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   TagBFP2-V5-HRAS G12V (HRAS Human, Entacmaea quadricolor (TagBFP))
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   TagBFP2-V5 tag

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)

   Plasmid

   #209078

   Purpose

   Ngn2 mediated cortical neuron induction, along with SNCA(3K) over-expression

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   SNCA (SNCA Human)
   NGN2 (Neurog2 Mouse)
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   3K mutant: E35K, E46K, E61K

   Promoter

   TRE4G

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

   Plasmid

   #209080

   Purpose

   Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   SNCA (SNCA Human)
   NGN2 (Neurog2 Mouse)
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   A53T

   Promoter

   TRE4G

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

   Plasmid

   #202553

   Purpose

   Nontargeting control vector with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Expression

   Mutation

   Puromycin-resistance gene in the pLV hU6-sgRNA hU…

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (VK_1124)

   Plasmid

   #209081

   Purpose

   Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-DeltaNAC-sfGFP over-expression

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   SNCA (SNCA Human)
   NGN2 (Neurog2 Mouse)
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   A53T, NAC deletion (aa60-95)

   Promoter

   TRE4G

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTKA001

   Plasmid

   #164577

   Purpose

   Expresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expression

   Depositor

   Robert Kirchdoerfer

   Article

   Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

   Insert

   nsp7 (ORF1ab )
   

   Use

   Tags

   6xHis, Ek, TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTKA002

   Plasmid

   #164578

   Purpose

   Expresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expression

   Depositor

   Robert Kirchdoerfer

   Article

   Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

   Insert

   nsp8
   

   Use

   Tags

   6xHis, Ek, TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223-SH3BP5

    Plasmid

    #23579

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    SH3BP5 (SH3BP5 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

11. control (pGS701)

    Plasmid

    #160037

    Purpose

    pLV-A4-UCOE-EF1-3xHA-GFP-MCS-IRES-BLAS-2A-SNAP-WPRE-A2

    Depositor

    David Sabatini

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT-puro

    Plasmid

    #17923

    Purpose

    Insect expression vector

    Depositor

    David Sabatini

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Drosophila metallothionein gene promoter drives e…

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. ER-M-TAG (pGS555)

    Plasmid

    #160040

    Purpose

    pLV-A4-UCOE-EF1a-MMGT1-mScarlet-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2

    Depositor

    David Sabatini

    Insert

    MMGT1-mScarlet-3xHA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. ER-P-TAG (pGS594)

    Plasmid

    #160039

    Purpose

    pLV-A4-UCOE-EF1a-CP450TMD-APEX2-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2

    Depositor

    David Sabatini

    Insert

    CP450TMD-APEX2-3xHA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. ER-J-TAG (pGS730)

    Plasmid

    #160038

    Purpose

    pLV-A4-UCOE-EF1-3xHA-sfGFP-JPH-TMD-IRES-BLAS-2A-SNAP-WPRE-A2

    Depositor

    David Sabatini

    Insert

    3xHA-sfGFP-JPH-TMD
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFA6a-link-mKO1-SpHis5

    Plasmid

    #44642

    Purpose

    Yeast tagging vector to create gene fusion with mKO1 with SpHis5 selection

    Depositor

    Kurt Thorn

    Insert

    mKO1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. myc mTOR kinase dead

    Plasmid

    #8482

    Depositor

    David Sabatini

    Insert

    mTOR (Mtor Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    D2357E V2364I

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFA6a-link-mKO1-CaUra3

    Plasmid

    #44638

    Purpose

    Yeast tagging vector to create gene fusion with mKO1 with CaUra3 selection

    Depositor

    Kurt Thorn

    Insert

    mKO1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. P9m

    Plasmid

    #120937

    Purpose

    MoClo golden gate assembly AB part for pOR1-OR2 (lambda cI-repressable strong promoter) - Please see Supplemental Documents for annotated Genbank file

    Depositor

    Richard Murray

    Insert

    Pr-OR1-OR2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT2-H3.1-WT-BFP

    Plasmid

    #209437

    Purpose

    SB-transposable plasmid expressing FLAGGED human histone mutant H3.1-Wt with BFP

    Depositor

    Maria Castro

    Insert

    H3.1-WT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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