161,873 results

1. pZT-AAVS1-L1

   Plasmid

   #52637

   Purpose

   TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

   Depositor

   Mahendra Rao, Jizhong Zou

   Article

   Luo et al Stem Cells Transl Med. 2014 May 15. pii:

   Insert

   Left AAVS1 TALEN
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti sgRNA(MS2)_puro backbone

   Plasmid

   #73795

   Purpose

   3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3 Promoter Vector

   Plasmid

   #212939

   Purpose

   Control vector with constitutive firefly luciferase expression

   Depositor

   Debrya Groskreutz

   Insert

   luc+
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   WT

   Promoter

   SV40

   Available since

   Jan. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pJKR-H-tetR

   Plasmid

   #62561

   Purpose

   Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.

   Depositor

   George Church

   Article

   Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti MS2-P65-HSF1_Hygro

   Plasmid

   #61426

   Purpose

   lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Insert

   MS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N55K in MS2

   Promoter

   EF1A

   Available since

   Dec. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDonor MCS Rosa26

   Plasmid

   #37200

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

   Inserts

   Rosa26 left targeting arm
   MCS
   Rosa26 right targeting arm
   

   Use

   Genomic targeting - zinc finger

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. GPI-RpHLuorin2

   Plasmid

   #171721

   Purpose

   GPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2 fused to GPI anchor
   

   Use

   Tags

   ratiometric pHluorin2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lamp1_mStayGold

   Plasmid

   #221902

   Purpose

   Expresses mStayGold fused to Lamp1

   Depositor

   Janelia Integrative Imaging

   Insert

   Lamp1_mStayGold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRX-IP-HaloTag7-LC3

   Plasmid

   #184899

   Purpose

   Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   LC3B
   

   Use

   Tags

   HaloTag7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-DDX3

    Plasmid

    #44975

    Depositor

    Robin Reed

    Article

    Lee et al Nucleic Acids Res. 2008 Aug;36(14):4708-

    Insert

    DDX3 (DDX3X Human)
    

    Use

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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