We narrowed to 165,567 results for: addgene

1. LC3B sgRNA

   Plasmid

   #207556

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the LC3B locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   CACTGAATACCAGCGCCTAG
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICH47742:FCP:Cas9YFP

   Plasmid

   #85986

   Purpose

   Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter

   Depositor

   Thomas Mock

   Article

   Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

   Insert

   Cas9
   

   Use

   CRISPR; Golden gate assembly

   Tags

   SV40 NLS, YFP

   Promoter

   FCP

   Available Since

   Jan. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICH47732:FCP:NAT

   Plasmid

   #85984

   Purpose

   Golden Gate Level 1 cassette encoding a Nat selectable marker under the FCP promoter

   Depositor

   Thomas Mock

   Article

   Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

   Insert

   NAT
   

   Use

   Golden gate assembly

   Promoter

   FCP

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV/hSyn-SCLM

   Plasmid

   #216760

   Purpose

   AAV2 transfer vector with the human synapsin I promoter for neuronal expression of SuperClomeleon

   Depositor

   George Augustine

   Insert

   SuperClomeleon
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   S30R and 11 AA deletion in the Cerulean CFP moiet…

   Promoter

   hSyn (human synapsin I)

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. ERoxBFP

   Plasmid

   #68126

   Purpose

   mammalian expression of ER localized oxBFP

   Depositor

   Erik Snapp

   Article

   Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

   Insert

   oxBFP
   

   Tags

   KDEL ER retention sequence and bovine prolactin s…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

6. ER-moxMaple3

   Plasmid

   #120885

   Purpose

   mammalian expression of ER localized moxMaple3

   Depositor

   Erik Snapp

   Article

   Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

   Insert

   moxMaple3
   

   Tags

   KDEL ER retention sequence and bovine prolactin s…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MXS_CMV::ZeoR-bGHpA

   Plasmid

   #62441

   Purpose

   MXS_chaining vector with CMV::ZeoR-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   resistance cassette against Zeocin with CMV Promoter
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. TALEN-mROSA26 ELD

   Plasmid

   #60026

   Purpose

   Expression of a TALEN protein specific for mouse ROSA26 locus.

   Depositor

   Radislav Sedláček

   Article

   Kasparek et al FEBS Lett. 2014 Sep 18. pii: S0014-5793(

   Insert

   TALEN-ROSA26
   

   Use

   TALEN

   Expression

   Mammalian

   Available Since

   Nov. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHACK-GAL4>QF2(newHA1)

   Plasmid

   #104873

   Purpose

   HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

   Depositor

   Christopher Potter

   Article

   Lin et al Genetics. 2016 Jun 22. pii: genetics.116

   Insert

   T2A-QF2
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-mRFP1-Human UTROPHIN_1-261

    Plasmid

    #225052

    Purpose

    Express in mammalian cells the mRFP1 fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.

    Depositor

    Pantelis Tsoulfas

    Insert

    Human Utrophin residues 1-261 and RFP1 (UTRN Human, Homo Sapiens)
    

    Use

    Lentiviral

    Tags

    Human UTROPHIN 1-261 and mRFP1

    Expression

    Mammalian

    Mutation

    Sequence is derived from ADDGENE Plasmid #26739-R…

    Promoter

    CMV promoter and enhancer

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-EGFP-Human-UTROPHIN_1-261

    Plasmid

    #222393

    Purpose

    Express in mammalian cells the EGFP fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.

    Depositor

    Pantelis Tsoulfas

    Insert

    Human Utrophin residues 1-261 and EGFP (UTRN Human, Homo Sapiens)
    

    Use

    Lentiviral

    Tags

    EGFP and Human UTROPHIN 1-261

    Expression

    Mammalian

    Mutation

    Sequence is derived from ADDGENE Plasmid #26737-G…

    Promoter

    CMV promoter and enhancer

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. MDC1 KO sgRNA

    Plasmid

    #207103

    Purpose

    pX330 based plasmid for expression of Cas9 and the GGTGTAACGTGGAGCCAGTA sgRNA to target the MDC1 coding sequence.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GGTGTAACGTGGAGCCAGTA
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-PGK-CXCR4 (neo)

    Plasmid

    #192077

    Purpose

    Lentivirus for expression of CXCR4

    Depositor

    Roland Friedel

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Lentiviral

    Promoter

    hPGK

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF-I-GFP GX

    Plasmid

    #45443

    Depositor

    John Brigande

    Article

    Gubbels et al Nature. 2008 Sep 25. 455(7212):537-41.

    Type

    Empty backbone

    Tags

    IRES EGFP

    Expression

    Mammalian

    Promoter

    human EF1α

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

15. p8267 LentiCRISPR v2 hygro sgNT-2

    Plasmid

    #193978

    Purpose

    Expression of spCas9 and non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9
    

    Use

    Lentiviral

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVPRT-tTR-KRAB

    Plasmid

    #11648

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Didier Trono, Patrick Aebischer

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    hPrion, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pdCas9-NED

    Plasmid

    #109358

    Purpose

    Mammalian vector for expressing effector domains fused to dCas9.

    Depositor

    Antal Kiss

    Article

    Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    dCas9, SV40 NLS, 3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX459_gRNA-AAVS1_hspCas9

    Plasmid

    #193309

    Purpose

    Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    not applicable

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-CarO-BFP2-TSERex

    Plasmid

    #124795

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    CarO-BFP2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-Mac-BFP2-TSERex

    Plasmid

    #124793

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    Mac-BFP2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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