161,641 results

1. 3XFlagNICD3

   Plasmid

   #20185

   Depositor

   Raphael Kopan

   Article

   Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

   Insert

   Notch 3 Intracellular Domain (Notch3 Mouse)
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Contains murine Notch 3 Intracellular Domain (Met…

   Promoter

   CMV

   Available since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-F in pAR-9

   Plasmid

   #166921

   Purpose

   AAV rep/cap plasmid. Capsid is mouse brain tropic AAV-F

   Depositor

   Casey Maguire

   Article

   Hanlon et al Mol Ther Methods Clin Dev. 2019 Oct 23;1

   Insert

   REP/CAP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. polyQ74-GFP

   Plasmid

   #231893

   Purpose

   Forms cytosolic HTT partial exon 1 Q74 aggregates

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   HTT partial exon 1 Q74 (HTT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   CAG repeat expansion

   Promoter

   Available since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pML107

   Plasmid

   #67639

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   single guide RNA expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pSNR52 and pTDH3 (aka GAP promoter)

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF1a-hMLH1dn

   Plasmid

   #174824

   Purpose

   Mammalian expression of human MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   human MLH1dn (codon optimized)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deletion of residues 754-756

   Promoter

   EF1a

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP Vinculin

   Plasmid

   #50513

   Depositor

   Kenneth Yamada

   Insert

   Vinculin (VCL Chicken)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGP-CMV-NES-jRGECO1a

   Plasmid

   #61563

   Purpose

   Red fluorescent calcium sensor protein

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Insert

   NES-jRGECO1a
   

   Use

   Tags

   nuclear export signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. tdTomato-N1

   Plasmid

   #54642

   Purpose

   Localization: N1 Cloning Vector, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson, Roger Tsien, Nathan Shaner

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. CRISPR-SB

   Plasmid

   #177936

   Purpose

   Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

   Depositor

   Roland Rad

   Article

   Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

   Type

   Empty backbone

   Use

   CRISPR; Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX551

    Plasmid

    #60957

    Purpose

    pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.

    Depositor

    Feng Zhang

    Article

    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

    Insert

    SpCas9
    

    Use

    AAV and CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    pMecp2

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-TLR4-YFP

    Plasmid

    #13018

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 4 (TLR4 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. DD-Cas9 with filler sequence and Venus (EDCPV)

    Plasmid

    #90085

    Purpose

    This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNA

    Depositor

    Raffaella Sordella

    Article

    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Destabilized Domain and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe B-Raf V600E

    Plasmid

    #17544

    Depositor

    Channing Der

    Article

    Hao et al Mol Cancer Ther. 2007 Aug . 6(8):2220-9.

    Insert

    B-Raf (BRAF Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    V600E

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2FLAG

    Plasmid

    #16331

    Depositor

    Peter Klein

    Insert

    Flag tag
    

    Use

    Vertebrate expression

    Tags

    Flag

    Expression

    Mutation

    Flag tag inserted into pCS2+ polylinker

    Promoter

    Available since

    Dec. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCJ136

    Plasmid

    #162665

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. gag/pol

    Plasmid

    #14887

    Depositor

    Tannishtha Reya

    Article

    Reya et al Nature. 2003 May 22. 423(6938):409-14.

    Insert

    gag / pol
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF0987

    Plasmid

    #142715

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KLF2 (KLF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+) Flag-His-ATM kd

    Plasmid

    #31986

    Depositor

    Michael Kastan

    Article

    Canman et al Science. 1998 Sep 11;281(5383):1677-9.

    Insert

    ATM kd (ATM Human)
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    3'UTRf 9 nucl/D2870A N2875k. catalytically …

    Promoter

    Available since

    March 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. eGFP-KIF5B

    Plasmid

    #172203

    Purpose

    Expresses KIF5B tagged with eGFP in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

    Insert

    KIF5B (Kif5b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. PCR4-Shh::lacZ-H11

    Plasmid

    #139098

    Purpose

    enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

    Depositor

    Len Pennacchio

    Article

    Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Shh

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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