We narrowed to 8,601 results for: Ott
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Plasmid#158205PurposeCassette of 10 different binding sites with high affinity to Qb phage coat protein, as extracted form the oligo pool experiment. It does not contain the Qb-wt sequence, therefore MCP cannot bind it.DepositorInsert10 binding sites of QB coat protein without the WT sequence based on the oligo library experiment
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-jph1a
Plasmid#109497PurposeMuscle specific zebrafish expression of mKate2 fused to junctophilin 1aDepositorInsertjph1a (jph1a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPlease see Depositor CommentsPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-ARF1-DN
Plasmid#109590PurposeMultisite gateway vector for 3' tagging with dominant negative ARF1DepositorInsertARF1 (arf1 Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdominant negativePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-ARF6-DN
Plasmid#109592PurposeMultisite gateway vector for 3' tagging with dominant negative ARF6DepositorInsertARF6 (arf6a Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdominant negativePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-SERCA1
Plasmid#109552PurposeMultisite gateway vector for 3' tagging with sarcoplasmic/endoplasmic calcium ATPase 1DepositorInsertsarcoplasmic/endoplasmic calcium ATPase 1 (atp2a1 Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-ARF1
Plasmid#109535PurposeMuscle specific zebrafish expression of mKate2 fused to ARF1DepositorInsertARF1 (arf1 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-Cav3-mKate2
Plasmid#109518PurposeMuscle specific zebrafish expression of mKate2 fused to Caveolin3DepositorInsertCav3 (cav3 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-Dnm2a-DN
Plasmid#109520PurposeMuscle specific zebrafish expression of mKate2 fused to dominant negative Dynamin2aDepositorInsertDnm2a (dnm2a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationDNPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AvicFP3
Plasmid#129506PurposeExpresses AvicFP3 constitutively in E. coli (most strains)DepositorInsertAvicFP3
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMS22
Plasmid#129710PurposeExpresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusionDepositorInsertEscherichia coli RssB-GSGS variant (rssB Escherichia coli MG1655)
UseTags10-Histidine tag cleavable with PreScission Prote…ExpressionBacterialMutationRssB E134G:E135S:E136G:E137SPromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
KL001: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-VPR
Plasmid#121836PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS/VPR scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sitesDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KL101: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-KRAB
Plasmid#121837PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS/KRAB scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sitesDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KL202: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-LSD1
Plasmid#121838PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS/LSD1 scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sitesDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KL401: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-Dnmt3a3L
Plasmid#121840PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS/Dnmt3a-3L scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sitesDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA
Plasmid#121803PurposepMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.DepositorInsertpolyA + EF1a::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IG806: pMVP (L3-L2) pA; RIP::eGFP-P2A-TETa-pA
Plasmid#121807PurposepMVP L3-L2 entry plasmid, contains polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven GFP-P2A-TETa downstream of gene of interest.DepositorInsertpolyA + RIP::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LF901: pMAGIC (L1-R5) mU6::SaCas9 gRNA scaffold
Plasmid#121811PurposepMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.DepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JI601: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; RIP
Plasmid#121815PurposepMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.DepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JI702: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; RIP
Plasmid#121820PurposepMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.DepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JH204: pMVP (R4-R3) human PDX1 open
Plasmid#121745PurposepMVP R4-R3 entry plasmid, contains human PDX1 (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertHuman PDX1 (open) (PDX1 Human)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IX702: pMVP (L3-L2) OLLAS-IRES-eGFP + polyA
Plasmid#121748PurposepMVP L3-L2 entry plasmid, contains OLLAS epitope tag + IRES2-eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion and IRES expression of eGFP reporterDepositorInsertOLLAS epitope tag-IRES2-eGFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-mClover-smAID-P2A-CD5Flag2-bglpA
Plasmid#110654PurposeLentiviral vector expressing cell surface Flag-tag and monomeric GFP C-terminaly fused with auxin inducible degronDepositorInsertmClover-AID-P2A-CD5Flag2-bglpA
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-mClover-smAID-P2A-CD5cmyc2-bglpA
Plasmid#110656PurposeLentiviral vector expressing cell surface c-Myc-tag and monomeric GFP C-terminaly fused with auxin inducible degronDepositorInsertmClover-AID-P2A-CD5cmyc2-bglpA
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEMS1942
Plasmid#105867PurposeHigh copy number plasmid containing Ple265 (PCP2 MiniPromoter) insertDepositorInsertPle265
UseTagsExpressionMutationPromoterPle265 (PCP2 MiniPromoter)Available sinceFeb. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMXs_FLAG-Sfxn2 (CG6812)
Plasmid#110643PurposeRetroviral expression of Flag-tagged Drosophila CG6812 in mammalian cellsDepositorInsertCG6812 (CG6812 Fly)
UseRetroviralTagsFLAGExpressionMammalianMutationcodon-optimized cDNA (no amino acid mutations)PromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-Sfxn1-3 (CG11739)
Plasmid#110642PurposeRetroviral expression of Flag-tagged Drosophila CG11739 in mammalian cellsDepositorInsertCG11739 (CG11739 Fly)
UseRetroviralTagsFLAGExpressionMammalianMutationcodon-optimized cDNA (no amino acid mutations)PromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEMS2235
Plasmid#111888PurposeHigh copy number plasmid containing Ple320 (POU4F1 MiniPromoter) insert.DepositorInsertPle320 (POU4F1 Human)
UseUnspecifiedTagsExpressionMutationPromoterPle320 (POU4F1 MiniPromoter)Available sinceAug. 10, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS2236
Plasmid#111889PurposeHigh copy number plasmid containing Ple321 (TUBB3 MiniPromoter) insert.DepositorInsertPle321 (TUBB3 Human)
UseUnspecifiedTagsExpressionMutationPromoterPle321 (TUBB3 MiniPromoter)Available sinceAug. 10, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSG5-chCAR
Plasmid#87231PurposeMammalian expression of chicken CARDepositorInsertCXADR (CXADR Chicken)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEMS2023
Plasmid#49130PurposeAAV plasmid with hs1218 promoter driving expression of iCre.DepositorInsertssAAV-hs1218-iCre
UseAAVTagsExpressionMammalianMutationPromoterhs1218Available sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS2024
Plasmid#49131PurposeAAV plasmid with hs671 promoter driving expression of iCre.DepositorInsertssAAV-hs671-iCre
UseAAVTagsExpressionMammalianMutationPromoterhs671Available sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS2025
Plasmid#49132PurposeAAV plasmid with NOV (Ple303) promoter driving expression of iCre.DepositorInsertssAAV-Ple303-iCre
UseAAVTagsExpressionMammalianMutationPromoterNOVAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS2050
Plasmid#49135PurposeAAV plasmid with CAGGS promoter driving expression of EmGFP.DepositorInsertssAAV-CAGGS-emGFP
UseAAVTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1(+)mGAT1-565-YFP-566CT
Plasmid#41677DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsYFPExpressionMammalianMutation“Monomerizing” YFP A206K mutation; YFP inserted d…PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1-570-CFP-571CT
Plasmid#41680DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsCFPExpressionMammalianMutation“Monomerizing” CFP A206K mutation; CFP inserted d…PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1-570-YFP-571CT
Plasmid#41679DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsYFPExpressionMammalianMutation“Monomerizing” YFP A206K mutation; YFP inserted d…PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1-565-CFP-566CT
Plasmid#41678DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsCFPExpressionMammalianMutation“Monomerizing” CFP A206K mutation; CFP inserted d…PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase delta 1278-1291
Plasmid#59830PurposeAllows the integration of myc Separase delta 1278-1291 in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
UseTagsMycExpressionMammalianMutationdelta 1278-1291Promoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Hspb3 in situ probe
Plasmid#45629DepositorInsertHspb3 in situ probe (Hspb3 Mouse)
UseIn situTagsExpressionMutationfragment contains nt 30-661 of Hspb3 mRNA sequenc…PromoterAvailable sinceJune 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1CFP45
Plasmid#41676DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsCFPExpressionMammalianMutation“Monomerizing” CFP A206K mutation; 45 C-terminal-…PromoterCMVAvailable sinceFeb. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1YFP45
Plasmid#41675DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsYFPExpressionMammalianMutation“Monomerizing” YFP A206K mutation; 45 C-terminal-…PromoterCMVAvailable sinceFeb. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA
Plasmid#61591PurposeA single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.DepositorInsertshSaCas9
Chimeric guide for SaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pStreptagII 3C SMO
Plasmid#234995PurposeExpression of a Strep-tag II labeled on its N-terminus receptor, with the Smoothened (Smo) receptorDepositorInsertSMO (SMO Human)
UseTagsStreptag-II, HRV 3C siteExpressionMammalianMutationPromoterAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEABR NA 3C FLAG SA
Plasmid#234987PurposeFor production of Extracellular Vesicles (EVs), with the transmembrane region of Neuraminidase protein fused to Streptavidin on the vesicles surfaceDepositorInsertEABR-NA
UseTagsHRV 3C site, FLAG, Strep-Tactin (SA)ExpressionMammalianMutationIn the Streptavidin coding sequence Glu44Val/Ser4…PromoterAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNA 3C myc scfvFLAG
Plasmid#234990PurposeExpression of transmembrane region of Neuraminidase protein fused to an anti FLAG scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG proteinDepositorInsertNA-3C-myc-scfvFLAG
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCold-I-Dsup
Plasmid#90021PurposeTo express His-tagged Dsup in bacteriaDepositorInsertDsup
UseTagsHisExpressionBacterialMutationPromotercspAAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSIN4-CMV-K2M
Plasmid#21164DepositorArticleUseLentiviral; ReprogrammingTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
EFIa-iTGB1
Plasmid#115799PurposeIntegrin B1 overexpressionDepositorInsertiTGB1 (ITGB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
RpH-ILV-3xALFA
Plasmid#225135PurposeRpH-ILV probe strongly colocalises with lysosomal markers.DepositorInsertTMEM192 (TMEM192 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-TRSter
Plasmid#78865PurposeExpresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS elementDepositorInsertThioredoxin Reductase (Txnrd1 Rat)
UseTagsMutated E. coli-type SECIS element (3’ end of ins…ExpressionBacterialMutationPromoterT7lacAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only