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pCMV-Tag2B EDD

Plasmid

#37188

Depositor

Darren Saunders, Charles Watts

Article

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD (UBR5 Human)

Tags

6x His Tag and FLAG

Expression

Mammalian

Mutation

Please see Depositor Comments

Promoter

CMV

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-monSTIM1

Plasmid

#161795

Purpose

Mammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)

Depositor

Won Do Heo

Article

Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

Insert

MonSTIM1 (STIM1 Mustard Weed, Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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p5E-gfap

Plasmid

#75024

Purpose

Can be used to drive expression in zebrafish astrocytes.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

glial fibrillary acidic protein promoter (gfap Zebrafish)

Use

5' entry vector for multisite gateway three …

Promoter

gfap

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti CMV-MRLC1-mRuby2-IRES-PuroR

Plasmid

#103031

Purpose

Lentiviral expression of mRuby2-tagged MRLC with puromycin selection

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

MRLC1 (MYL9 Human)

Use

Lentiviral

Tags

mRuby2

Promoter

CMV immediate early

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-Flag-PGC1a-6His

Plasmid

#67637

Purpose

AAV vector expressing PGC1a gene

Depositor

Connie Cepko

Article

Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

Insert

PGC1a (Ppargc1a Mouse)

Use

AAV

Tags

6XHis and Flag tag

Promoter

CMV

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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EYFP-p65

Plasmid

#111192

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

Insert

RELA (RELA Human)

Tags

EYFP

Expression

Mammalian

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-HA-Nr4a1

Plasmid

#160941

Purpose

Generation of retrovirus for the overexpression of Nr4a1

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 (Nr4a1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pINSPECT_SigP:NLuc

Plasmid

#197263

Purpose

Cloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

Type

Empty backbone

Use

CRISPR, Luciferase, Synthetic Biology, and Unspec…

Expression

Mammalian

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM1-Flag

Plasmid

#180406

Purpose

lentiviral transduction of human TGM1 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM1 (TGM1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro CXCR4 siRNA-2

Plasmid

#12272

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

CXCR4 siRNA (CXCR4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pGLS3-5xHRE-p53(K164R)

Plasmid

#72555

Purpose

Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K164R.

Depositor

Ester Hammond

Article

Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

Insert

p53 (TP53 Human)

Tags

5XHRE

Expression

Mammalian

Mutation

K164R

Promoter

E1B minimal promoter

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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mCardinal-Keratin-17

Plasmid

#56164

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Keratin (KRT18 Human)

Tags

mCardinal

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hsyn-Jaws-GFP-ER2

Plasmid

#65016

Purpose

AAV-mediated expression of Jaws-ER2 under the human synapsin promoter

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-GFP-ER2

Use

AAV

Tags

ER2 and GFP

Expression

Mammalian

Mutation

K200R W214F

Promoter

human synapsin

Available Since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro CXCR4 siRNA-1

Plasmid

#12271

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

CXCR4 siRNA (CXCR4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pPB.DEST-ITGB1(WT)-mRuby2

Plasmid

#215448

Purpose

Piggybac expression vector with integrin beta1 tagged with mRuby2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Transposon-based stable expression

Tags

mRuby2

Expression

Mammalian

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

CAG

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pHIV-INK4A-mOrange2

Plasmid

#110730

Purpose

Mammalian Expression of p16INK4A IRES H2B-mOrange2

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

p16INK4A IRES H2B-mOrange2 (CDKN2A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pRetroX SgK269 WT

Plasmid

#68028

Purpose

expression of Sgk269 in mammalian cells

Depositor

Roger Daly

Article

Croucher et al Cancer Res. 2013 Mar 15;73(6):1969-80. d

Insert

PEAK1 (PEAK1 Human)

Use

Retroviral

Tags

FLAG/myc, HA, and IRES-DsRed

Expression

Mammalian

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pEN35-CDKN2A-Ex2-R

Plasmid

#110737

Purpose

Encodes Cas9 nickase and gRNA targeting CDKN2A Exon2

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

CDKN2A gRNA (CDKN2A Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX301-ARF6-TagRFP

Plasmid

#162029

Purpose

Expression of tagged ARF WT

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF6 (ARF6 Human)

Use

Lentiviral

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEN35-CDKN2A-Ex2-L

Plasmid

#110736

Purpose

Encodes Cas9 nickase and gRNA targeting CDKN2A Exon2

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

CDKN2A gRNA (CDKN2A Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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