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pCSC-SP-PW-Nep (aka: pBOB-NEP)

Plasmid

#12338

Depositor

Inder Verma

Article

Marr et al J Mol Neurosci. 2004 . 22(1-2):5-11.

Insert

NEP (MME Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pCAG-PDZD8HA

Plasmid

#105005

Purpose

Mammalian Expression of PDZD8

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

PDZD8 (Pdzd8 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

Plasmid

#158652

Purpose

making transgenics expressing a UAS driven eGFP and NTR 2.0

Depositor

Jeff Mumm

Article

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Mutation

F70A/F108Y

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF1a-GATA1-IRES-Puro

Plasmid

#61062

Purpose

lentiviral vector for GATA1

Depositor

Igor Slukvin

Article

Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

Insert

GATA1 (GATA1 Human)

Use

Lentiviral

Tags

Flag

Promoter

EF1alpha

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-M-Flag-BAP1

Plasmid

#125840

Purpose

express wild type BAP1

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7 EGFPC2 TAZ

Plasmid

#66850

Purpose

Express GFP-fused TAZ by lentivirus

Depositor

Yutaka Hata

Article

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Insert

WWTR1/TAZ (WWTR1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagD 121L

Plasmid

#19309

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Tags

GST and HA

Expression

Mammalian

Mutation

Q121L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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HA-FOXO3a WT DBM (H212R)

Plasmid

#8352

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

FOXO3a (FOXO3 Human)

Tags

HA

Expression

Mammalian

Mutation

H212R

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pRPF215

Plasmid

#106377

Purpose

C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon

Depositor

Robert Fagan, Neil Fairweather

Article

Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1

Insert

Ptet-himar1-Ter(slpA)-transposon

Use

E. coli - c. difficile shuttle vector

Mutation

Codon optimised for C. difficile

Promoter

Ptet

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCMX/cyclinB1 (17)

Plasmid

#26060

Depositor

Jonathon Pines

Article

Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

Insert

Cyclin B1 (CCNB1 Human)

Tags

Myc

Expression

Mammalian

Available Since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only
PKC epsilon WT

Plasmid

#21240

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC epsilon (Prkce Mouse)

Tags

HA

Expression

Mammalian

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
pCMV HA hRb delta CDK

Plasmid

#58906

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

all 15 Ser/Thr Cdk acceptor sites changed to Ala,…

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA4 HisMax-V5-GFP-RRBP1

Plasmid

#92150

Purpose

Expresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

RRBP1 (RRBP1 Human)

Tags

Calreticulin signal peptide, EGFP (A207K mutation…

Expression

Mammalian

Mutation

In RRBP1: N951S and T1329L. E1279 is also missing.

Promoter

CMV

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4-ERSE2-luc2P-Hygro

Plasmid

#101790

Purpose

Luciferase reporter plasmid containing three tandem repeats of the ER stress response element-II (ERSE-II)

Depositor

Seiichi Oyadomari

Article

Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

Insert

3xERSE-II

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

minimal TATA-box promoter with low basal activity

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-HA-EIF3S5

Plasmid

#22546

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

EIF3S5 (EIF3F Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pMCSF-R-luc

Plasmid

#12420

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1994 Jan . 14(1):373-81.

Insert

M-CSF receptor promoter fragment (CSF1R Human)

Use

Luciferase

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pET15-UbE2W

Plasmid

#15809

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2W (UBE2W Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-iCreV

Plasmid

#140135

Purpose

Can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Has Service

AAV PHP.eB

Insert

iCreV

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pRS424GFP-2xPH(Osh2)

Plasmid

#36095

Depositor

Scott Emr

Article

Stefan et al Cell. 2011 Feb 4;144(3):389-401.

Insert

2xPH(Osh2) fusion

Tags

GFP

Expression

Yeast

Promoter

PRC1

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
GFP-SENP3

Plasmid

#34554

Depositor

Mary Dasso

Article

Yun et al J Cell Biol. 2008 Nov 17;183(4):589-95.

Insert

SENP3 (SENP3 Human)

Tags

EGFP

Promoter

CMV

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

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