165,947 results

1. pB80-hsKIF5B(1-353)GCN4-L-GFP

   Plasmid

   #193714

   Purpose

   Expression of dimeric, GFP tagged, human kinesin-1 (KIF5B aa1-353)

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-353)-GCN4-L-GFP (KIF5B Human, Synthetic)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-353);

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB80-hsKIF5B(1-353)-GCN4-L-mCherry

   Plasmid

   #193715

   Purpose

   Expression of dimeric, mCherry tagged, human kinesin-1 (KIF5B aa1-353)

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-353)-GCN4-L-mCherry (KIF5B Human, Synthetic)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-353); mCherry: Met1 Deleted

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB

   Plasmid

   #193719

   Purpose

   Rapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transport

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-560)Rigor-L-2xHA-L-FRB (KIF5B Human, Synthetic)
   

   Tags

   2xHA-FRB

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-560), RIGOR: G234A

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB80-hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro)

   Plasmid

   #193721

   Purpose

   Optogenetic coupling to dimeric human kinesin-1 (KIF5B aa1-807) via SSPB(micro) for anterograde transport

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro) (KIF5B Human, Synthetic)
   

   Tags

   mVenus-SSPB(micro)

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-MBP-ATG9 (692-839)

   Plasmid

   #188092

   Purpose

   To make monomeric ATG9 C-terminal tail for expression in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 (ATG9A Human)
   

   Tags

   MBP tag

   Expression

   Mammalian

   Mutation

   ATG9 (692-839)

   Promoter

   CMV

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM33_YES1_SH3

   Plasmid

   #178492

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   YES1_SH3 (YES1 Human, Synthetic)
   

   Tags

   His-GST

   Expression

   Bacterial

   Promoter

   T7/LacO

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmr101A-cPr-mNeonGreen (ErmR)

   Plasmid

   #177208

   Purpose

   Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

   Depositor

   Alexey Merz

   Insert

   mNeonGreen
   

   Expression

   Bacterial

   Mutation

   Yeast codon optimized

   Promoter

   Synthetic constitutive promoter

   Available Since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDY153: Flag(SUZ12) WT HDR plasmid

   Plasmid

   #170793

   Purpose

   WT homology directed repair plasmid for performing genome-editing on the SUZ12 Locus

   Depositor

   Thomas Cech

   Article

   Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

   Insert

   SUZ12 (SUZ12 Human)
   

   Expression

   Mammalian

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDY156: SUZ12 px330 sgRNA Cas9 plasmid

   Plasmid

   #170792

   Purpose

   This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

   Depositor

   Thomas Cech

   Article

   Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

   Insert

   Cas9
   

   Expression

   Mammalian

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+) eGFP SH3BP5 G4

    Plasmid

    #129022

    Purpose

    Expresses an eGFP mRNA with the SH3BP5 G-quadruplex (G4) region in the 3' UTR

    Depositor

    Jeremy Wilusz

    Article

    Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Insert

    eGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENN.AAV.hSyn.Cre.WPRE.hGH

    Plasmid

    #105553

    Purpose

    AAV expression of Cre from hSyn promoter

    Depositor

    James M. Wilson

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9_T2A_mCherry sg1-sg2

    Plasmid

    #192479

    Purpose

    All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Tags

    T2A mCherry

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209722

    Purpose

    Expresses mitochondrially targeted low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (mito).iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pComb3XTT

    Plasmid

    #63891

    Purpose

    Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

    Depositor

    Carlos Barbas

    Article

    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

    Insert

    TT Fab
    

    Use

    Phage display

    Tags

    6xHis, HA tag, and geneIII

    Expression

    Bacterial

    Promoter

    lacZ

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. wtBxb1

    Plasmid

    #222337

    Purpose

    Plasmid for expressing wild type Bxb1 in mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized wild type Bxb1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCE-hSK

    Plasmid

    #41814

    Purpose

    Non-integrating (episomal) expression of human SOX2 and KLF4

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    SOX2, KLF4 (SOX2, KLF4 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCE-hUL

    Plasmid

    #41855

    Purpose

    Non-integrating (episomal) expression of human L-MYC and LIN28

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    L-MYC, LIN28 (MYCL, LIN28A Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBiFC-VN173

    Plasmid

    #22010

    Purpose

    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    VN173
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Venus residus 1-172

    Available Since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. T4 td upstream intron-PABP

    Plasmid

    #189972

    Purpose

    Part 1 plasmid to introduce the T4 td upstream intron and PABP spacer into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    T4 td upstream intron-PABP
    

    Use

    Cloning

    Promoter

    N/A

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. HBA1 full 3'UTR-T4 td downstream intron

    Plasmid

    #189974

    Purpose

    Part 6 plasmid to introduce the HBA1 full 3'UTR and T4 td downstream intron into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    HBA1 full 3'UTR-T4 td downstream intron
    

    Use

    Cloning

    Promoter

    N/A

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge