We narrowed to 24,628 results for: Nes

1. pJK422

   Plasmid

   #155373

   Purpose

   dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   Available Since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK423

   Plasmid

   #155374

   Purpose

   dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   Available Since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TB:3xDBE_RE:MiniP

   Plasmid

   #124529

   Purpose

   3 Copies of the Daf-16 family binding element

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   3 copies of the Daf-16 family binding element
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Antares-SEPLuc IRESv24

   Plasmid

   #141087

   Purpose

   precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   Antares-T2A-puromycin-IRESv24-SEPLuc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pVAX_CBI_AcrIIA6_DT1

   Plasmid

   #136668

   Purpose

   Expresses human codon-optimized AcrIIA6 DT1 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

   Depositor

   Yannick Doyon

   Article

   Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

   Insert

   AcrIIA6_DT1
   

   Use

   Synthetic Biology

   Tags

   No tag, No NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pVAX_CBI_AcrIIA6_D1811

   Plasmid

   #136667

   Purpose

   Expresses human codon-optimized AcrIIA6 D1811 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

   Depositor

   Yannick Doyon

   Article

   Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

   Insert

   AcrIIA6_D1811
   

   Use

   Synthetic Biology

   Tags

   No tag, No NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pVAX_CBI_AcrIIA6_D1024

   Plasmid

   #136666

   Purpose

   Expresses human codon-optimized AcrIIA6 D1024 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

   Depositor

   Yannick Doyon

   Article

   Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

   Insert

   AcrIIA6_D1024
   

   Use

   Synthetic Biology

   Tags

   No tag, No NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pVAX_CBI_AcrIIA5_D4276

   Plasmid

   #136664

   Purpose

   Expresses human codon-optimized AcrIIA5 D4276 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

   Depositor

   Yannick Doyon

   Article

   Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

   Insert

   AcrIIA5_D4276
   

   Use

   Synthetic Biology

   Tags

   No tag, No NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pVAX_CBI_AcrIIA5_D1126

   Plasmid

   #136663

   Purpose

   Expresses human codon-optimized AcrIIA5 D1126 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

   Depositor

   Yannick Doyon

   Article

   Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

   Insert

   AcrIIA5_D1126
   

   Use

   Synthetic Biology

   Tags

   No tag, No NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC 3GLA

    Plasmid

    #71762

    Purpose

    gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant

    Depositor

    Matthias Soller

    Article

    Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS315Nab3D191stop

    Plasmid

    #110537

    Purpose

    Expresses yeast NAB3 using the endogenous promoter

    Depositor

    Daniel Reines

    Article

    Loya et al PLoS One. 2017 Oct 12;12(10):e0186187. d

    Insert

    NAB3 (NAB3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C-terminal deltion of 191 amino acids

    Promoter

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-mus-Bnip3_deltaExon3

    Plasmid

    #100793

    Purpose

    Mammalian expression of Bnip3_deltaExon3 splice variant

    Depositor

    Joseph Gordon

    Article

    Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

    Insert

    mouse-Bnip3_deltaExon3 (Bnip3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Exon3 deletion

    Promoter

    CMV

    Available Since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. p415TEF-NYFP

    Plasmid

    #99555

    Purpose

    Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

    Depositor

    Liming Li

    Article

    Hines et al PLoS Genet. 2011 Feb;7(2):e1001309. doi:

    Insert

    Swi1 N-rich region (AA1-327) (SWI1 Budding Yeast)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR221-WNT1

    Plasmid

    #79499

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT1 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    WNT1 (WNT1 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSTAP-seq_fly-ssp3

    Plasmid

    #86382

    Purpose

    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster ssp3 enhancer
    

    Use

    Stap-seq screening vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSTAP-seq_fly-ham

    Plasmid

    #86383

    Purpose

    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster ham enhancer
    

    Use

    Stap-seq screening vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSTAP-seq_fly-sgl

    Plasmid

    #86384

    Purpose

    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster sgl enhancer
    

    Use

    Stap-seq screening vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSTAP-seq_fly-ncm

    Plasmid

    #86385

    Purpose

    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster ncm enhancer
    

    Use

    Stap-seq screening vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDGB1alpha1_SF (GB0106)

    Plasmid

    #68232

    Purpose

    Twister plasmid to swap inserts from an alpha2 or alpha2R vector to any omega level vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Solanum lycopersicum intergenic region
    

    Use

    Synthetic Biology; Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTAct2 (GB0210)

    Plasmid

    #68191

    Purpose

    Provides the Arabidopsis thaliana Actin2 gene terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtAct2 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPMtb (GB0080)

    Plasmid

    #68167

    Purpose

    Provides the S. Lycopersicum metallothionein II-like protein (MTB) gene promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SlMtb promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPTctp (GB0083)

    Plasmid

    #68169

    Purpose

    Provides the S. Lycopersicum translationally-controlled tumor protein homolog promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SlTCTP promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p5'UTRAct2 (GB0193)

    Plasmid

    #68178

    Purpose

    Provides the 5'-untranslated region of the Arabidopsis thaliana actin2 gene as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    5'UTR AtAct2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEGB 1alfa1R Tnos:Hygromycin:PNos (GB0235)

    Plasmid

    #68213

    Purpose

    Transcriptional unit for Hygromycin resistance gene plant expression

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Hyg
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDGB1omega1_SF (GB0158)

    Plasmid

    #68242

    Purpose

    Twister plasmid to swap inserts from an omega2 or omega2R vector to any alpha level vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Solanum lycopersicum intergenic region
    

    Use

    Synthetic Biology; Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMyb12 (GB0075)

    Plasmid

    #68199

    Purpose

    Provides the CDS of theSolanum lycopersicum Myb12 transcription factor as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Myb12 coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPAt2S3 (GB0029)

    Plasmid

    #68162

    Purpose

    Provides the Arabidopsis thaliana Seed-specific promoter 2S3 as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    At2S3 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUPD_Mini35s(-60,UTR) (GB0050)

    Plasmid

    #68176

    Purpose

    Provides the CaMV Mini35S Promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    CaMV Mini35S promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Y344C

    Plasmid

    #24433

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A, Y344C

    Promoter

    Available Since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

30. L4C/S330C

    Plasmid

    #24436

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A,, L4C/S3…

    Promoter

    Available Since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

31. K222C

    Plasmid

    #24438

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A, K222C

    Promoter

    Available Since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

32. L4C

    Plasmid

    #24437

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A, L4C

    Promoter

    Available Since

    May 5, 2010

    Availability

    Academic Institutions and Nonprofits only

33. S330C

    Plasmid

    #24439

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A, "S…

    Promoter

    Available Since

    May 5, 2010

    Availability

    Academic Institutions and Nonprofits only

34. K339CLM S330C

    Plasmid

    #24441

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S, S330C

    Promoter

    Available Since

    May 5, 2010

    Availability

    Academic Institutions and Nonprofits only

35. K343CLM A337C

    Plasmid

    #24442

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S, A337C

    Promoter

    Available Since

    May 5, 2010

    Availability

    Academic Institutions and Nonprofits only

36. pcFNE3A (chick fibronectin E3A)

    Plasmid

    #14062

    Depositor

    Richard Hynes

    Article

    Norton et al Mol Cell Biol. 1987 Dec . 7(12):4297-307

    Insert

    Chicken Fibronectin EIIIA (FN1 Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Blunted BstE2-AvaII fragment was inserted into bl…

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

37. UAS-u(L)Cas9

    Plasmid

    #127385

    Purpose

    Expresses Cas9 at low level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF(L)-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLXSN_HPV16E7∆DLYC

    Plasmid

    #136588

    Purpose

    Retroviral vector for expression of RB-binding mutant of HPVE7

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    HPV16E7∆DLYC
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    del aa 21-24, full length is 1-98

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLX302 GDF11-V5 puro

    Plasmid

    #83097

    Purpose

    Lentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    GDF11 (GDF11 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

40. 6xHis-SNAP-PI(3)P Probe

    Plasmid

    #211508

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

    Depositor

    David Murray, Hannes Maib

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    FYVE-Hrs x 2 (PIKFYVE Human)
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge