We narrowed to 4,345 results for: Ado;

1. pcDNA3.1 PA-mCit-BAD

   Plasmid

   #113459

   Purpose

   ProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1)

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Insert

   BCL2 associated agonist of cell death (BAD Human)
   

   Use

   Tags

   ProteinA-mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPaint-mScarlet-PuroR [M1G]

   Plasmid

   #171809

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. JDW 836 (pCS2-V5-mScarlet-I-hsKRAS4A-WT)

   Plasmid

   #156411

   Purpose

   pCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human wild type KRAS4A-WT (KRAS Human)
   

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-PGK-RAP1A-E63-neo

   Plasmid

   #156174

   Purpose

   Lentiviral vector for expression of RAP1A-E63, with neomycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1A (RAP1A Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   changed Glutamine 63 to Glutamic acid

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFGC-I2Cas9

   Plasmid

   #173158

   Purpose

   Expresses Cas9 in dividing Arabidopsis cells. Contains dsRED and Basta for fast transformant selection.

   Depositor

   Piotr Ziolkowski

   Article

   Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

   Inserts

   hSpCas9
   pNAP:dsRED:tNOS
   pMAS:BAR:tMAS
   ICU2 upstream regulatory region
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Plant

   Mutation

   Contains a D169N mutation with no visible effect …

   Promoter

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. iMb-Notch-Mosaic (IR99.40)

   Plasmid

   #99749

   Purpose

   Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteins and Notch signalling genes

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   MbYFP, MbTomato, MbKate2, DN-Rbpj and NICD-PEST
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. JDW 763 (pCS2-EYFP-hsKRAS4B-WT)

   Plasmid

   #156408

   Purpose

   pCS2 CMV expression vector driving EYFP fused human KRAS4B

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human wild type KRAS4B-WT (KRAS Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCJ211

   Plasmid

   #162684

   Purpose

   pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with deletion of the predicted signal peptide (Lys2:Gly19), with C-terminal His tag, codon optimized for expression in E. coli K12.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) without 19 residue signal peptide
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   deltaK2:G19; codon optimized for expression in E.…

   Promoter

   T7/lac

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR D-TOPO-C3_9

   Plasmid

   #60258

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   NKX6-1 enhancer (NKX6-1 Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL4.23 C3_25

    Plasmid

    #60305

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    ROCK1 enhancer (ROCK1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pXPR_BRD003 Luciferase

    Plasmid

    #117072

    Purpose

    single guide RNA targeting Luciferase

    Depositor

    William Hahn

    Article

    Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

    Insert

    Luciferase
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pInducer20_SLC38A2_wt

    Plasmid

    #156182

    Purpose

    Dox-inducible expression of sgRNA-resistant SLC38A2 wild-type cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA: T…

    Promoter

    TRE

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-PGK-RAP2A-V12-neo

    Plasmid

    #156178

    Purpose

    Lentiviral vector for expression of RAP2A-V12, with neomycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-PGK-flag-RAP1B-V12-neo

    Plasmid

    #156175

    Purpose

    Lentiviral vector for expression of flag-RAP1B-V12, with neomycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1B (RAP1B Bovine)
    

    Use

    Lentiviral

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-PGK-flag-RAP1B-N17-neo

    Plasmid

    #156176

    Purpose

    Lentiviral vector for expression of flag-RAP1B-N17, with neomycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1B (RAP1B Bovine)
    

    Use

    Lentiviral

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    changed Serine 17 to Asparagine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-PGK-RAP2A-N17-neo

    Plasmid

    #156179

    Purpose

    Lentiviral vector for expression of RAP2A-N17, with neomycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Serine 17 to Asparagine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL4.23 C3_9

    Plasmid

    #60295

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    NKX6-1 enhancer (NKX6-1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pLentiCMVblast_SLC38A2_N82A

    Plasmid

    #156181

    Purpose

    CMV-driven expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA: T…

    Promoter

    CMV

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pInducer20_SLC38A2_N82A

    Plasmid

    #156183

    Purpose

    Dox-inducible expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA: T…

    Promoter

    TRE

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)

    Plasmid

    #156405

    Purpose

    Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codon

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human wild type KRAS4A-WT (KRAS Human)
    

    Use

    Gateway cloning

    Tags

    mScarlet-I

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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