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Showing: 2041 - 2060 of 19882 results

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pET26b_Nb.b201

Plasmid

#131404

Purpose

E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin

Depositor

Andrew Kruse

Publication

McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

Insert

Nanobody b201

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Promoter

T7

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pACYC-FLAG-dN6-His

Plasmid

#22143

Depositor

Robert Sauer

Publication

Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

Insert

Flag-clpXdeltaNLinkedHexamer-His6

Tags

Flag, His6, and L20 Linkers

Expression

Bacterial

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSBbi_RP_MicroID2-HA

Plasmid

#188521

Purpose

Encodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cells

Depositor

James Londino

Publication

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

MicroID2

Use

Transposon mediated gene integration

Tags

HA

Expression

Mammalian

Promoter

EF1a/RPBSA

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLncEXP

Plasmid

#64865

Purpose

Empty vector for lncRNA expression

Depositor

Lei Sun

Publication

Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

Type

Empty backbone

Use

Retroviral

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pHLS-EF1a-FRB-SpCas9-A

Plasmid

#138477

Purpose

Expresses FRB fused SpCas9 for NanoMEDIC packaging.

Depositor

Akitsu Hotta

Publication

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

SpCas9, human codon optimized

Use

CRISPR

Tags

3x HA tag and FRB

Expression

Mammalian

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LeGO-iT

Plasmid

#27361

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

U6 promoter, SFFV promoter, IRES-tdTomato

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFPC1-hVAP-A KD/MD

Plasmid

#104449

Purpose

Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Publication

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Tags

EGFP

Expression

Mammalian

Mutation

changed K94 to D and M96 to D

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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CMV-FLAG-Core-R (pMO29)

Plasmid

#24480

Depositor

Melanie Ott

Publication

Schwer et al J Virol. 2004 Aug . 78(15):7958-68.

Insert

Core

Tags

FLAG

Expression

Mammalian

Available since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pHN781

Plasmid

#178828

Purpose

Bacterial expression for human Saposin A

Depositor

Kazuhiro Abe

Insert

artificial gene encoding human Saposin A (AR Human)

Tags

His8, TEV

Expression

Bacterial

Mutation

Codon-usage was optimized for bacterial expressio…

Promoter

T7

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP-SHP2CS

Plasmid

#12284

Depositor

Anton Bennett

Publication

Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

Insert

SHP-2CS (PTPN11 Human)

Tags

Myc

Expression

Mammalian

Mutation

Cysteine 459 mutated to Serine

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMXspuro-GFP-NBR1

Plasmid

#74202

Purpose

Retroviral expression of GFP-NBR1

Depositor

Jayanta Debnath

Publication

Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

Insert

neighbor of BRCA1 gene 1 (NBR1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pPAICS-EGFP

Plasmid

#99108

Purpose

Expresses human PAICS-EGFP fusion in mammalian cells

Depositor

Stephen Benkovic

Publication

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

PAICS (PAICS Human)

Tags

EGFP (enhanced green fluorescent protein)

Expression

Mammalian

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Tagged-IRES

Plasmid

#35570

Depositor

Marian Waterman

Publication

Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

Insert

Lymphoid Enhancer Factor 1 5'UTR (LEF1 Human)

Use

Luciferase

Tags

Firefly Luciferase and MS2 Stem Loop

Expression

Mammalian

Promoter

SV40

Available since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHairyRed

Plasmid

#69709

Purpose

Fluorescent reporter of Agrobacterium rhizogenes mediated transformed roots

Depositor

Brett Ferguson, Peter Gresshoff

Publication

Lin et al Mol Plant. 2011 May;4(3):537-45. doi: 10

Type

Empty backbone

Expression

Plant

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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T-2A-EGFP-PGK-Puro

Plasmid

#83344

Purpose

Donor vector to knocin EGFP into human Brachyury(T) locus

Depositor

James Thomson

Publication

Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

Insert

Human Brachyury knockin homology arms (TBXT Human)

Tags

2A-EGFP

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET30ax

Plasmid

#85761

Purpose

A bacterial expression vector with a His-tag at N and C-terminus.

Depositor

Lei Lu

Publication

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Tags

His tag (N-term and C-term)

Expression

Bacterial

Promoter

T7 promoter

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-MSCV-hCD38-EF1α-GFP+Puro

Plasmid

#134936

Purpose

expression human CD38 in mammalian cells

Depositor

Jianjun Zhao

Publication

Hu et al Cancer Res. 2020 Mar 19. pii: 0008-5472.

Insert

CD38 (CD38 Human)

Use

Lentiviral

Expression

Mammalian

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEZY19

Plasmid

#18668

Purpose

pET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site.

Depositor

Yu-Zhu Zhang

Publication

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Use

Gateway Cloning

Tags

His

Expression

Bacterial

Promoter

T7

Available since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEYFP-N1-A2BR

Plasmid

#37202

Depositor

Robert Tarran

Publication

Watson et al FASEB J. 2011 Sep;25(9):2996-3003. Epub

Insert

A2BR (ADORA2B Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pMM146

Plasmid

#127218

Purpose

BeYDV replicon with constitutive Luc expression, BBM, sgRNA targeting PDS

Depositor

Daniel Voytas

Publication

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, BBM, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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