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Flag-Gsdmd-NT 4A

Plasmid

#80952

Purpose

Expresses Gsdmd-NT 4A mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT 4A (Gsdmd Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpA

Plasmid

#65455

Purpose

Can be used to generate AAV virus that will express EGFP from the CAG promoter under intersectional control by Flp and Cre recombinases

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

EGFP

Use

AAV

Promoter

CAG

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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mScarlet-EEA1

Plasmid

#169067

Purpose

Express human EEA1 in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

EEA1 (EEA1 Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 luciferase

Plasmid

#26612

Depositor

Jie Chen

Article

Kim et al Proc Natl Acad Sci U S A. 2000 Dec 19. 9

Insert

firefly luciferase

Expression

Mammalian

Available Since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only
pCE048-SiT-Cas12a

Plasmid

#128124

Purpose

Express both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pSBbi G418R mbIL21

Plasmid

#194329

Purpose

Consitiutive mammalian expression of membrane bound human IL-21

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Insert

mbIL-21 (IL21 Human)

Expression

Mammalian

Promoter

EF1-a

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRK6-HA-TAK1

Plasmid

#44160

Depositor

Xin Lin

Article

Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

Insert

Tak1 (Map3k7 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only
PKC beta II DN

Plasmid

#16385

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta II (Prkcb Mouse)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative (kinase inactive): K371R

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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pQE9-His-p97(wt)

Plasmid

#14666

Depositor

Graham Warren

Article

Meyer et al EMBO J. 2000 May 15. 19(10):2181-92.

Insert

p97 (Vcp Mouse)

Tags

RGS6xHis

Expression

Bacterial

Mutation

See depositor comments below.

Available Since

April 13, 2007

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-let-7 8x

Plasmid

#20931

Depositor

Yukihide Tomari

Article

Iwasaki et al Mol Cell. 2009 Apr 10;34(1):58-67. doi:

Insert

8x let-7 target sites

Use

Luciferase

Expression

Insect and Mammalian

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pQE30-His-AlaRS

Plasmid

#124103

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Alanyl-tRNA ligase (alaS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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hE-cadherin/Δβ-catenin-pcDNA3

Plasmid

#45772

Depositor

Barry Gumbiner

Article

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

hE-cadherin/Δβ-catenin (CDH1 Human)

Expression

Mammalian

Mutation

Ends at amino acid 844. Last 35 amino acids dele…

Promoter

CMV

Available Since

June 14, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-NRF1

Plasmid

#34707

Depositor

Raymond Deshaies

Article

Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

Insert

NRF1 (NFE2L1 Human)

Use

Retroviral

Tags

3XFlag

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
pRint

Plasmid

#24218

Depositor

Mariano Garcia-Blanco

Article

Bonano et al Nat Protoc. 2007 . 2(9):2166-81.

Insert

RFP with modified adeno intron

Expression

Mammalian

Mutation

dsRED interrupted by a modified adenovirus intron

Available Since

March 30, 2010

Availability

Academic Institutions and Nonprofits only

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GFP-Rnd3

Plasmid

#23229

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

Rnd3 (RND3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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pYPQ133A

Plasmid

#69275

Purpose

Golden Gate entry vector; 3rd gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-hROSA26-1-1kbdonor

Plasmid

#234736

Purpose

PseCAST crRNA targeting hROSA26-1, with 1 kb donor transposon

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

hROSA26-1 crRNA

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET-Sfp

Plasmid

#159617

Purpose

Expression plasmid for Sfp phosphopantetheinyl transferase

Depositor

Christopher T Walsh, Jun Yin

Article

Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46.

Insert

Sfp

Tags

6HIS

Expression

Bacterial

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Nano-lantern-plasma membrane/pcDNA3

Plasmid

#51975

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membrane

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Tags

HIS and Lyn

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pLNCX2-mCherry-VPS4A

Plasmid

#115334

Purpose

expresses mCherry-VPS4A in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

VPS4A (VPS4A Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

silent mutation in position 4050 C to T

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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