We narrowed to 59,329 results for: cloning

1. Hs.HRAS C186S

   Plasmid

   #83189

   Purpose

   Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), C186S mutation

   Depositor

   Dominic Esposito

   Insert

   HRAS (HRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   C186S

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGGB003

   Plasmid

   #48821

   Purpose

   Provides a default dummy as GreenGate N-terminal tag module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   B-Dummy
   

   Use

   Golden gate compatible cloning vector

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. 599 p3E-mcs1

   Plasmid

   #49004

   Purpose

   Multiple cloning site in 3' gateway entry vector

   Depositor

   Nathan Lawson

   Article

   Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

   Type

   Empty backbone

   Use

   Gateway 3' entry

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCZGY2849

   Plasmid

   #74112

   Purpose

   Enhanced mutant version of membrane targeted PH-miniSOG (Q103L) entry clone for more efficient ablation of cells when expressed under desired promoter.

   Depositor

   Andrew Chisholm

   Article

   Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

   Insert

   miniSOG(Q103L)
   

   Use

   Gateway topo cloning

   Tags

   PH

   Available Since

   April 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYEXNH3

   Plasmid

   #49024

   Purpose

   FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag and 3C protease cleavage site

   Depositor

   Eric Geertsma, Raimund Dutzler

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Tags

   3C protease site (PreScission site) and decaHis (…

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME eos

   Plasmid

   #73792

   Purpose

   Photoconvertible eos in a middle entry clone

   Depositor

   David Raible

   Article

   Prendergast et al Development. 2012 Mar;139(6):1141-52. do

   Insert

   EOS
   

   Use

   Middle entry clone

   Available Since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. sgPal7 (p2Tol-U6-sgPal7-HygR)

   Plasmid

   #71484

   Purpose

   Expresses palindromic sgRNA for use in self-cloning CRISPR

   Depositor

   Richard Sherwood

   Article

   Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

   Insert

   sgPal7
   

   Promoter

   U6

   Available Since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. MXS_E2-Crimson

   Plasmid

   #62410

   Purpose

   MXS_chaining vector with E2-Crimson

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   E2-Crimson
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHHYTK113

   Plasmid

   #200144

   Purpose

   Type 4a part for Modular Cloning in Yeast

   Depositor

   Johannes Herrmann

   Article

   Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

   Insert

   sfGFP1-10
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGGF005

    Plasmid

    #48846

    Purpose

    Provides a hygromycin resistance cassette as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    pUBQ10::HygromycinR::tOCS
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICXNHG3

    Plasmid

    #49021

    Purpose

    FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGGD002

    Plasmid

    #48834

    Purpose

    Provides a default dummy as GreenGate C-terminal tag module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    D-Dummy
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Hs.HRAS Q61R

    Plasmid

    #83188

    Purpose

    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), Q61R mutation

    Depositor

    Dominic Esposito

    Insert

    HRAS (HRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61R

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Hs.HRAS G12D

    Plasmid

    #83183

    Purpose

    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), G12D mutation

    Depositor

    Dominic Esposito

    Insert

    HRAS (HRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12D

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pL2-(GGS)4GSG

    Plasmid

    #137099

    Purpose

    iFLinkC Linker cloning

    Depositor

    Viktor Stein

    Article

    Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

    Insert

    (GGS)4GSG
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGGA006

    Plasmid

    #48816

    Purpose

    Provides the UBQ10 promoter as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    UBQ10 promoter
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. MXS_P2A

    Plasmid

    #62433

    Purpose

    MXS_chaining vector with P2A

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    Self-cleaving peptide
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. p5E-ESyn1

    Plasmid

    #80804

    Purpose

    hSyn1 promoter fused to CMVmin

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    p5E-ESyn1
    

    Expression

    Mammalian

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDXC3GMS

    Plasmid

    #49031

    Purpose

    FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, EGFP, Myc tag and streptavidin binding peptide

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site), EGFP, MYC, a…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pL2-G(P)10G

    Plasmid

    #137105

    Purpose

    iFLinkC Linker cloning

    Depositor

    Viktor Stein

    Article

    Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

    Insert

    G(P)10G
    

    Use

    Synthetic Biology

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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