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pscAAV-CAG-RLuc

Plasmid

#83280

Purpose

recombinant AAV vector packaging self-complementary Renilla Luciferase under the CAG promoter

Depositor

Mark Kay

Publication

Paulk et al Mol Ther Methods Clin Dev. 2018 Jul 24;1

Insert

Renilla Luciferase

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFH3.3_Cre-MS2x12

Plasmid

#205559

Purpose

Express Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Publication

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Cre

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-B19N

Plasmid

#59924

Purpose

Expression vector for Rabies virus SAD B19 nucleoprotein gene

Depositor

Ian Wickersham

Insert

Rabies virus nucleoprotein

Expression

Mammalian

Promoter

CAG

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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V5-LOV-Turbo-NES

Plasmid

#199545

Purpose

expresses LOV-Turbo in the mammalian cytosol, lentiviral vector

Depositor

Alice Ting

Publication

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

NES and V5

Expression

Mammalian

Promoter

TRE3G

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mRNA-CleanCap-NanoLuc

Plasmid

#188247

Purpose

Template plasmid to transcribe mRNA encoding NanoLuc

Depositor

Howard Chang

Publication

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

NanoLuc

Use

Luciferase

Expression

Mammalian

Promoter

T7

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-TIWB-yellow pre-eGRASP(p32)

Plasmid

#111582

Purpose

An AAV vector that expresses yellow pre-eGRASP(p32) under the tetO promoter.

Depositor

Bong-Kiun Kaang

Publication

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

yellow pre-eGRASP(p32)

Use

AAV

Promoter

tetO

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBAD-LSSmCherry1

Plasmid

#89986

Purpose

Long stokes-shift variant of red fluorescent protein mCherry

Depositor

Robert Campbell

Publication

Shen et al PLoS One. 2017 Feb 27;12(2):e0171257. do

Insert

LSSmCherry1

Tags

6xHis-T7-Xpress

Expression

Bacterial

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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lenti SYN-dCas9-KRAB-MeCP2

Plasmid

#155365

Purpose

Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

Depositor

Jeremy Day

Publication

Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

Insert

dCas9-KRAB-MeCP2

Use

CRISPR and Lentiviral

Tags

3x FLAG and 7x His

Expression

Mammalian

Promoter

SYN

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBabe-TetCMV-puro-mVenus-LOV

Plasmid

#22033

Purpose

Mammalian expression of mVenus tagged LOV2 domain

Depositor

Klaus Hahn

Publication

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-LOV

Use

Retroviral

Expression

Mammalian

Mutation

Insert length based on Venus (720bp) + LOV (450bp)

Available since

Sept. 11, 2009

Availability

Academic Institutions and Nonprofits only
pMSCV_IRES_GFP_CD16a

Plasmid

#196189

Purpose

Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

Depositor

Ana Ruiz-Saenz

Publication

Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

Insert

CD16a (FCGR3A Human)

Use

Retroviral

Expression

Mammalian

Mutation

changed Phenylalanine 158 to Valine

Promoter

LTR

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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LentiGuide-TagRFP-2A-BSD

Plasmid

#167930

Purpose

Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

Depositor

Ronald Germain

Publication

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1_Floxed-STOP Fluc

Plasmid

#122962

Purpose

Firefly luciferase (Fluc)-based bioluminescent reporter for Cre-loxP DNA recombination reaction

Depositor

Moritoshi Sato

Publication

Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

Insert

pcDNA3.1_Floxed-STOP Fluc

Use

Cre/Lox and Luciferase

Expression

Mammalian

Promoter

CMV

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTEF2

Plasmid

#43971

Depositor

Hal Alper

Publication

Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Promoter

TEF

Available since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1(+)- e18

Plasmid

#131670

Purpose

Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.

Depositor

Alberto Ciccia

Publication

Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

Insert

e18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-rtTA

Plasmid

#175274

Purpose

AAV vector mediating neuron-specific expression of rtTA

Depositor

Wei Xu

Publication

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Has service

AAV1

Insert

Reverse tetracycline-controlled transactivator (rtTA)

Use

AAV

Promoter

Synapsin

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pACYC-Skp-TF

Plasmid

#83921

Purpose

Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Publication

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Trigger Factor

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pJW1311

Plasmid

#61254

Purpose

Template for cloning sgRNA(F+E) to generate new PU6::sgRNAs by PCR-fusion

Depositor

Jordan Ward

Publication

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

sgRNA(F+E) targeting Y61A9L9.1

Use

CRISPR; Cloning template

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mRNA-CleanCap-mNeonGreen

Plasmid

#188248

Purpose

Template plasmid to transcribe mRNA encoding mNeonGreen

Depositor

Howard Chang

Publication

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Expression

Mammalian

Promoter

T7

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pZ149-pcDNA3-BhCas12b_v4

Plasmid

#122446

Purpose

Expresses BhCas12b_v4 in mammalian cells

Depositor

Feng Zhang

Publication

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

CMV-NLS-BhCas12b_v4-NLS-3xHA

Expression

Mammalian

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHR_AXP_hrGFP

Plasmid

#84613

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locus

Depositor

Ian Wheeldon

Publication

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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