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We narrowed to 2,116 results for: PORA

Showing: 2061 - 2080 of 2116 results

  1. pBA904

    Plasmid
    #122238
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122238-plasmid-map-sequence-id-292011

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  2. pBA900

    Plasmid
    #122237
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs2 at the 3' end of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    122237-plasmid-map-sequence-id-292010

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  4. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid
    #50831
    Purpose
    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells
    Depositor
    Peter Schultz
    Article
    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11
    Inserts
    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    Use
    Baculoviral
    Tags
    His, Myc-6xHis, and WPRE
    Expression
    Insect
    Mutation
    Y39TAG and able to charge various unnatural amino…
    Promoter
    CAG, CMV, and U6 and H1
    Available since
    June 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    50831-plasmid-map-sequence-id-193569

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  5. pCMV_NESPylRS(AF)_hU6tRNAPyl

    Plasmid
    #223508
    Purpose
    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5
    Inserts
    NES Pyrrolysyl-tRNA Synthetase (AF) (pylS Methanosarcina mazei)
    tRNA Pyl
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Y306A; Y384F
    Promoter
    CVM, SP6 and U6
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223508-plasmid-map-sequence-id-445174

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  6. pcDNA5 FRT/TO SF-HERC2 (ShB-R)

    Plasmid
    #55613
    Purpose
    Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cells
    Depositor
    David Chan
    Article
    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.
    Insert
    3xFLAG tagged HERC2 (WT) (HERC2 Human)
    Use
    Tags
    3xFLAG and STREP
    Expression
    Mammalian
    Mutation
    Silent mutations have been incorporated into the …
    Promoter
    Available since
    July 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    55613-plasmid-map-sequence-id-86499

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  7. pCMV_NESPylRS(WT)_hU6tRNAPyl

    Plasmid
    #223509
    Purpose
    PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5
    Inserts
    NES Pyrrolysyl-tRNA Synthetase (pylS Methanosarcina mazei)
    tRNA Pyl
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, SP6 and U6
    Available since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    223509-plasmid-map-sequence-id-448627

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  9. pRaGE Pyl TAG GFP Y35TAG

    Plasmid
    #160041
    Purpose
    Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.
    Depositor
    Lital Alfonta
    Article
    Ozer et al Front Bioeng Biotechnol. 2021 Feb 26;9:5
    Inserts
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    deGFP
    Use
    Synthetic Biology
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed tyrosine 35 to TAG stop-codon (Site numbe…
    Promoter
    P70b promoter and T500 terminator, Vibrio natrieg…
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160041-plasmid-map-sequence-id-332393

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  11. LgBiT-CYBC1:CYBB-SmBiT BiBiT Vector(CMV)

    Plasmid
    #237084
    Purpose
    Express LgBiT-CYBC1:CYBB-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Inserts
    CYBC1 (CYBC1 Human)
    CYBB (CYBB Human)
    Use
    Tags
    LgBiT and SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237084-plasmid-map-sequence-id-473313

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  12. NanoBRET PD-1/SHP2 Bidirectional Vector

    Plasmid
    #237016
    Purpose
    Express NanoLuc(R)-PD1/SHP2-HaloTag Fusion Proteins in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Inserts
    PD-1 (PTPN11 Human)
    SHP2 (PTPN11 Human)
    Use
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237016-plasmid-map-sequence-id-471879

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  14. NanoLuc-NRAS (Q61R)-CRAF RBD-HaloTag BiBRET Vector

    Plasmid
    #236862
    Purpose
    Express NanoLuc(R)-NRAS (Q61R)-CRAF RBD-HaloTag(R) in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Inserts
    NRAS (NRAS Human)
    cRAF(RBD) (RAF1 Human)
    Use
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    Q61R
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236862-plasmid-map-sequence-id-471854

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  16. BiBRET Vector HaloTag-NRAS (Q61R)-BRAF RBD-NanoLuc

    Plasmid
    #236852
    Purpose
    Express HaloTag(R)-NRAS (Q61R)-BRAF RBD-NanoLuc(R) in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Inserts
    NRAS (NRAS Human)
    BRAF (RBD) (BRAF Human)
    Use
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    Q61R
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236852-plasmid-map-sequence-id-471850

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  17. pRCA860 - pBA904 Puro-T2A-GFP EGR3 g1 CRISPRa guide guide (pRCA360 backbone)

    Plasmid
    #238188
    Purpose
    Lentiviral CRISPR guide vector expressing a EGR3 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
    Depositor
    Thomas Norman
    Article
    Southard et al Nat Genet (2025)
    Insert
    EGR3 sgRNA CRISPRa (EGR3 Human, Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238188-plasmid-map-sequence-id-478351

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  18. pRCA805 - pBA904 Puro-T2A-GFP KLF4 g1 CRISPRa guide (pRCA360 backbone) 668

    Plasmid
    #238174
    Purpose
    Lentiviral CRISPR guide vector expressing a KLF4 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
    Depositor
    Thomas Norman
    Article
    Southard et al Nat Genet (2025)
    Insert
    KLF4 sgRNA CRISPRa (KLF4 Human, Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    238174-plasmid-map-sequence-id-474465

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  19. pRCA803 - pBA904 Puro-T2A-GFP KLF5 g1 CRISPRa guide (pRCA360 backbone) 666

    Plasmid
    #238172
    Purpose
    Lentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
    Depositor
    Thomas Norman
    Article
    Southard et al Nat Genet (2025)
    Insert
    KLF5 sgRNA CRISPRa (KLF5 Human, Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    238172-plasmid-map-sequence-id-474457

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  20. pRCA804 - pBA904 Puro-T2A-GFP KLF5 g2 CRISPRa guide (pRCA360 backbone) 665

    Plasmid
    #238173
    Purpose
    Lentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
    Depositor
    Thomas Norman
    Article
    Southard et al Nat Genet (2025)
    Insert
    KLF5 sgRNA CRISPRa (KLF5 Human, Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    238173-plasmid-map-sequence-id-474456

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Showing: 2061 - 2080 of 2116 results